TY  - JOUR
AU  - Cabrera-Barjas, Gustavo
AU  - Albornoz, Karin
AU  - Belchi, Maria Dolores Lopez
AU  - Giordano, Ady
AU  - Bravo-Arrepol, Gaston
AU  - Moya-Elizondo, Ernesto
AU  - Martin, Juan San
AU  - Valdes, Oscar
AU  - Nešić, Aleksandra
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12870
AB  - In this work, chitosan films loaded with gallic acid and different content of chitin nanofibers were prepared and subjected to different characterization techniques. The results showed that the inclusion of gallic acid to chitosan films caused moderate decrease in water vapor permeability (by 29 %) and increased tensile strength of films (by 169 %) in comparison to the neat chitosan films. Furthermore, it was found that the addition of chitin nanofibers up to 30 % into chitosan/gallic acid films additionally improved tensile strength (by 474 %) and reduced plasticity of films (by 171 %), when compared to the chitosan/gallic acid films. Increased concentration of chitin nanofibers in films reduced the overall water vapor permeability of films by 51 %. In addition, gallic acid and chitin nanofibers had synergic effect on high chitosan film's antioxidant and antifungal activity toward Botrytis cinerea (both above 95 %). Finally, chitosan/gallic acid/chitin nanofibers films reduced decay incidence of strawberries, increased total soluble solid content, and promoted high production of some polyphenols during cold storage, in comparison to the control chitosan films and uncoated strawberry samples. Hence, these results suggest that chitosan/gallic acid/chitin nanofibers can present eco-sustainable approach for preservation of strawberries, giving them additional nutritional value.
T2  - International Journal of Biological Macromolecules
T1  - Influence of chitin nanofibers and gallic acid on physical-chemical and biological performances of chitosan-based films
VL  - 263, Part 1
SP  - 130159
DO  - 10.1016/j.ijbiomac.2024.130159
ER  - 

@article{
author = "Cabrera-Barjas, Gustavo and Albornoz, Karin and Belchi, Maria Dolores Lopez and Giordano, Ady and Bravo-Arrepol, Gaston and Moya-Elizondo, Ernesto and Martin, Juan San and Valdes, Oscar and Nešić, Aleksandra",
year = "2024",
abstract = "In this work, chitosan films loaded with gallic acid and different content of chitin nanofibers were prepared and subjected to different characterization techniques. The results showed that the inclusion of gallic acid to chitosan films caused moderate decrease in water vapor permeability (by 29 %) and increased tensile strength of films (by 169 %) in comparison to the neat chitosan films. Furthermore, it was found that the addition of chitin nanofibers up to 30 % into chitosan/gallic acid films additionally improved tensile strength (by 474 %) and reduced plasticity of films (by 171 %), when compared to the chitosan/gallic acid films. Increased concentration of chitin nanofibers in films reduced the overall water vapor permeability of films by 51 %. In addition, gallic acid and chitin nanofibers had synergic effect on high chitosan film's antioxidant and antifungal activity toward Botrytis cinerea (both above 95 %). Finally, chitosan/gallic acid/chitin nanofibers films reduced decay incidence of strawberries, increased total soluble solid content, and promoted high production of some polyphenols during cold storage, in comparison to the control chitosan films and uncoated strawberry samples. Hence, these results suggest that chitosan/gallic acid/chitin nanofibers can present eco-sustainable approach for preservation of strawberries, giving them additional nutritional value.",
journal = "International Journal of Biological Macromolecules",
title = "Influence of chitin nanofibers and gallic acid on physical-chemical and biological performances of chitosan-based films",
volume = "263, Part 1",
pages = "130159",
doi = "10.1016/j.ijbiomac.2024.130159"
}

Cabrera-Barjas, G., Albornoz, K., Belchi, M. D. L., Giordano, A., Bravo-Arrepol, G., Moya-Elizondo, E., Martin, J. S., Valdes, O.,& Nešić, A.. (2024). Influence of chitin nanofibers and gallic acid on physical-chemical and biological performances of chitosan-based films. in International Journal of Biological Macromolecules, 263, Part 1, 130159.
https://doi.org/10.1016/j.ijbiomac.2024.130159

Cabrera-Barjas G, Albornoz K, Belchi MDL, Giordano A, Bravo-Arrepol G, Moya-Elizondo E, Martin JS, Valdes O, Nešić A. Influence of chitin nanofibers and gallic acid on physical-chemical and biological performances of chitosan-based films. in International Journal of Biological Macromolecules. 2024;263, Part 1:130159.
doi:10.1016/j.ijbiomac.2024.130159 .

Cabrera-Barjas, Gustavo, Albornoz, Karin, Belchi, Maria Dolores Lopez, Giordano, Ady, Bravo-Arrepol, Gaston, Moya-Elizondo, Ernesto, Martin, Juan San, Valdes, Oscar, Nešić, Aleksandra, "Influence of chitin nanofibers and gallic acid on physical-chemical and biological performances of chitosan-based films" in International Journal of Biological Macromolecules, 263, Part 1 (2024):130159,
https://doi.org/10.1016/j.ijbiomac.2024.130159 . .

TY  - JOUR
AU  - Giordano, Ady
AU  - Morales-Tapia, Pablo
AU  - Moncada-Basualto, Mauricio
AU  - Pozo-Martínez, Josué
AU  - Olea-Azar, Claudio
AU  - Nešić, Aleksandra
AU  - Cabrera-Barjas, Gustavo
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10130
AB  - Plant biochemistry studies have increased in recent years due to their potential to improve human health. Argylia radiata is an extremophile plant with an interesting polyphenolic profile. However, its biomass is scarce and occasionally available. Argylia in vitro biomass was obtained from tissue culture and compared with in vivo roots regarding its polyphenolic and flavonoid content. Different solvents were used to prepare extracts from the in vitro tissue of callus and aerial plant organs and in vivo roots. UPLC-MS/MS was used to assess the chemical composition of each extract. ORAC-FL and scavenging of free radicals (DPPH and OH) methods were used to determine the antioxidant capacity of extracts. Furthermore, the biological activity of the extracts was established using the cellular antioxidant activity method. The vitroplants were a good source of polyphenols (25–68 mg GAE/100 g tissue FW), and methanol was the most efficient solvent. Eight polyphenolic compounds were identified, and their antioxidant properties were investigated by different chemical methods with EPR demonstrating its specific scavenging activity against free radicals. All extracts showed cellular dose-dependent antioxidant activity. The methanolic extract of vitroplants showed the highest cellular antioxidant activity (44.6% and 51%) at 1 and 10 µg/mL of extract, respectively. Vitroplants of A. radiata are proposed as a biotechnological product as a source of antioxidant compounds with multiple applications.
T2  - Molecules
T1  - Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots
VL  - 27
IS  - 3
SP  - 610
DO  - 10.3390/molecules27030610
ER  - 

@article{
author = "Giordano, Ady and Morales-Tapia, Pablo and Moncada-Basualto, Mauricio and Pozo-Martínez, Josué and Olea-Azar, Claudio and Nešić, Aleksandra and Cabrera-Barjas, Gustavo",
year = "2022",
abstract = "Plant biochemistry studies have increased in recent years due to their potential to improve human health. Argylia radiata is an extremophile plant with an interesting polyphenolic profile. However, its biomass is scarce and occasionally available. Argylia in vitro biomass was obtained from tissue culture and compared with in vivo roots regarding its polyphenolic and flavonoid content. Different solvents were used to prepare extracts from the in vitro tissue of callus and aerial plant organs and in vivo roots. UPLC-MS/MS was used to assess the chemical composition of each extract. ORAC-FL and scavenging of free radicals (DPPH and OH) methods were used to determine the antioxidant capacity of extracts. Furthermore, the biological activity of the extracts was established using the cellular antioxidant activity method. The vitroplants were a good source of polyphenols (25–68 mg GAE/100 g tissue FW), and methanol was the most efficient solvent. Eight polyphenolic compounds were identified, and their antioxidant properties were investigated by different chemical methods with EPR demonstrating its specific scavenging activity against free radicals. All extracts showed cellular dose-dependent antioxidant activity. The methanolic extract of vitroplants showed the highest cellular antioxidant activity (44.6% and 51%) at 1 and 10 µg/mL of extract, respectively. Vitroplants of A. radiata are proposed as a biotechnological product as a source of antioxidant compounds with multiple applications.",
journal = "Molecules",
title = "Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots",
volume = "27",
number = "3",
pages = "610",
doi = "10.3390/molecules27030610"
}

Giordano, A., Morales-Tapia, P., Moncada-Basualto, M., Pozo-Martínez, J., Olea-Azar, C., Nešić, A.,& Cabrera-Barjas, G.. (2022). Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots. in Molecules, 27(3), 610.
https://doi.org/10.3390/molecules27030610

Giordano A, Morales-Tapia P, Moncada-Basualto M, Pozo-Martínez J, Olea-Azar C, Nešić A, Cabrera-Barjas G. Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots. in Molecules. 2022;27(3):610.
doi:10.3390/molecules27030610 .

Giordano, Ady, Morales-Tapia, Pablo, Moncada-Basualto, Mauricio, Pozo-Martínez, Josué, Olea-Azar, Claudio, Nešić, Aleksandra, Cabrera-Barjas, Gustavo, "Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots" in Molecules, 27, no. 3 (2022):610,
https://doi.org/10.3390/molecules27030610 . .

TY  - JOUR
AU  - Cabrera-Barjas, Gustavo
AU  - Quezada, Adriana
AU  - Bernardo, Yamilé
AU  - Moncada, Mauricio
AU  - Zúñiga, Elisa
AU  - Wilkens, Marcela
AU  - Giordano, Ady
AU  - Nešić, Aleksandra
AU  - Delgado, Nacarid
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8845
AB  - Murta (Ugni molinae Turcz) is an endemic Chilean specie mostly used in medical, cosmetic, and food industries. However, during the industrial processing of murta fruits, the biomass containing the seeds is discarded as an industrial byproduct that does not find significant uses yet. This work is a first approach to valorize murta biomass through the identification and quantification of principal chemical constituents and exploring their antibacterial properties. The proximal analysis revealed that murta seeds exhibited significant content of raw fiber (64%), crude fat (14%), crude protein (12%), and low levels of ashes (1.5%) and minerals (0.04–0.23%). Dietary fiber was mainly composed of lignin, cellulose, pectin, and hemicellulose. Polyunsaturated fatty acids (89.0%), monounsaturated fatty acids (7.7%), and saturated fatty acids (3.3%) were the main constituents of seed oils. The arginine, asparagine, glutamic acid, and glycine were the primary protein constituent amino acids. Tannin fractions, total polyphenolic content, and oxygen radical absorbance capacity as antioxidant activity were measured. The chromatographic and mass spectrometric analysis (HPLC–MS/MS) confirmed the presence of several phenolic compounds like phenolic acids, flavonols, flavones, proanthocyanidins, and high molecular weight polyphenols. The murta seed extract showed high antibacterial activity against both Gram positive (Staphylococcus aureus, Bacillus cereus, and Streptococcus pyogenes) and Gram negative (Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa) bacterial strains. Murta seeds could be considered as a new source of nutritional components and bioactive compounds for different nutraceutical and food applications. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Journal of Food Measurement and Characterization
T1  - Chemical composition and antibacterial activity of red murta (Ugni molinae Turcz.) seeds: an undervalued Chilean resource
VL  - 14
IS  - 4
SP  - 1810
EP  - 1821
DO  - 10.1007/s11694-020-00428-x
ER  - 

@article{
author = "Cabrera-Barjas, Gustavo and Quezada, Adriana and Bernardo, Yamilé and Moncada, Mauricio and Zúñiga, Elisa and Wilkens, Marcela and Giordano, Ady and Nešić, Aleksandra and Delgado, Nacarid",
year = "2020",
abstract = "Murta (Ugni molinae Turcz) is an endemic Chilean specie mostly used in medical, cosmetic, and food industries. However, during the industrial processing of murta fruits, the biomass containing the seeds is discarded as an industrial byproduct that does not find significant uses yet. This work is a first approach to valorize murta biomass through the identification and quantification of principal chemical constituents and exploring their antibacterial properties. The proximal analysis revealed that murta seeds exhibited significant content of raw fiber (64%), crude fat (14%), crude protein (12%), and low levels of ashes (1.5%) and minerals (0.04–0.23%). Dietary fiber was mainly composed of lignin, cellulose, pectin, and hemicellulose. Polyunsaturated fatty acids (89.0%), monounsaturated fatty acids (7.7%), and saturated fatty acids (3.3%) were the main constituents of seed oils. The arginine, asparagine, glutamic acid, and glycine were the primary protein constituent amino acids. Tannin fractions, total polyphenolic content, and oxygen radical absorbance capacity as antioxidant activity were measured. The chromatographic and mass spectrometric analysis (HPLC–MS/MS) confirmed the presence of several phenolic compounds like phenolic acids, flavonols, flavones, proanthocyanidins, and high molecular weight polyphenols. The murta seed extract showed high antibacterial activity against both Gram positive (Staphylococcus aureus, Bacillus cereus, and Streptococcus pyogenes) and Gram negative (Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa) bacterial strains. Murta seeds could be considered as a new source of nutritional components and bioactive compounds for different nutraceutical and food applications. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Journal of Food Measurement and Characterization",
title = "Chemical composition and antibacterial activity of red murta (Ugni molinae Turcz.) seeds: an undervalued Chilean resource",
volume = "14",
number = "4",
pages = "1810-1821",
doi = "10.1007/s11694-020-00428-x"
}

Cabrera-Barjas, G., Quezada, A., Bernardo, Y., Moncada, M., Zúñiga, E., Wilkens, M., Giordano, A., Nešić, A.,& Delgado, N.. (2020). Chemical composition and antibacterial activity of red murta (Ugni molinae Turcz.) seeds: an undervalued Chilean resource. in Journal of Food Measurement and Characterization, 14(4), 1810-1821.
https://doi.org/10.1007/s11694-020-00428-x

Cabrera-Barjas G, Quezada A, Bernardo Y, Moncada M, Zúñiga E, Wilkens M, Giordano A, Nešić A, Delgado N. Chemical composition and antibacterial activity of red murta (Ugni molinae Turcz.) seeds: an undervalued Chilean resource. in Journal of Food Measurement and Characterization. 2020;14(4):1810-1821.
doi:10.1007/s11694-020-00428-x .

Cabrera-Barjas, Gustavo, Quezada, Adriana, Bernardo, Yamilé, Moncada, Mauricio, Zúñiga, Elisa, Wilkens, Marcela, Giordano, Ady, Nešić, Aleksandra, Delgado, Nacarid, "Chemical composition and antibacterial activity of red murta (Ugni molinae Turcz.) seeds: an undervalued Chilean resource" in Journal of Food Measurement and Characterization, 14, no. 4 (2020):1810-1821,
https://doi.org/10.1007/s11694-020-00428-x . .