TY  - JOUR
AU  - Milićević, Zorka T.
AU  - Bogojevic, D.
AU  - Mihailovic, M.
AU  - Petrović, Milica S.
AU  - Krivokapic, Z.
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3456
AB  - A key role of hsp90 in the activity of various oncogenic proteins and pathways is currently of intense interest. To clarify the molecular basis of biological behaviour of colorectal cancers we analysed the expression characteristics of hsp90 in cytosolic, nuclear and plasma membranous fractions of cancer cells. As determined by Western blot assay all hsp90 isoforms studied, a (84 kDa), 8 (86 kDa) and hsp90N (75 kDa), were up-regulated and differentially expressed in various stages of colorectal carcinoma. The inducible hsp90 alpha isoform is a component of invasive phenotype of cancer cells thus pointing to the importance of hsp90 alpha for metastasis generation. The expression of hsp90 beta is definitely higher in poorly-differentiated carcinomas than in well-differentiated cancers, suggesting an involvement of hsp90 beta in the inhibition of cancer cell differentiation. Especially, the expression of cytosolic hsp90N isoform in malignant cells points to the possibility that induction or overexpression of hsp90N might be causally related to tumour formation. Hsp90N is the plasma-membrane-associated protein in poorly-differentiated colorectal cancers with metastasis. This suggests that the expression of hsp90N is elevated with progressive dedifferentiation often associated with advanced cancer stages. Hsp90 was exclusively localized in the invasive front in a majority of metastatic cancers as visualized by immunohistochemical study. Consistent with these facts, the frequent expression of hsp90 alpha and hsp90N on the surface of colorectal cancer cells may enable hsp90 to act as a mediator of metastasis generation. The above results indicate more complex roles for hsp90 in colorectal tumourigenesis. In this way, the hsp90 would be at the crossroads of both signalling and cell migration events.
T2  - International Journal of Oncology
T1  - Molecular characterization of hsp90 isoforms in colorectal cancer cells and its association with tumour progression
VL  - 32
IS  - 6
SP  - 1169
EP  - 1178
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3456
ER  - 

@article{
author = "Milićević, Zorka T. and Bogojevic, D. and Mihailovic, M. and Petrović, Milica S. and Krivokapic, Z.",
year = "2008",
abstract = "A key role of hsp90 in the activity of various oncogenic proteins and pathways is currently of intense interest. To clarify the molecular basis of biological behaviour of colorectal cancers we analysed the expression characteristics of hsp90 in cytosolic, nuclear and plasma membranous fractions of cancer cells. As determined by Western blot assay all hsp90 isoforms studied, a (84 kDa), 8 (86 kDa) and hsp90N (75 kDa), were up-regulated and differentially expressed in various stages of colorectal carcinoma. The inducible hsp90 alpha isoform is a component of invasive phenotype of cancer cells thus pointing to the importance of hsp90 alpha for metastasis generation. The expression of hsp90 beta is definitely higher in poorly-differentiated carcinomas than in well-differentiated cancers, suggesting an involvement of hsp90 beta in the inhibition of cancer cell differentiation. Especially, the expression of cytosolic hsp90N isoform in malignant cells points to the possibility that induction or overexpression of hsp90N might be causally related to tumour formation. Hsp90N is the plasma-membrane-associated protein in poorly-differentiated colorectal cancers with metastasis. This suggests that the expression of hsp90N is elevated with progressive dedifferentiation often associated with advanced cancer stages. Hsp90 was exclusively localized in the invasive front in a majority of metastatic cancers as visualized by immunohistochemical study. Consistent with these facts, the frequent expression of hsp90 alpha and hsp90N on the surface of colorectal cancer cells may enable hsp90 to act as a mediator of metastasis generation. The above results indicate more complex roles for hsp90 in colorectal tumourigenesis. In this way, the hsp90 would be at the crossroads of both signalling and cell migration events.",
journal = "International Journal of Oncology",
title = "Molecular characterization of hsp90 isoforms in colorectal cancer cells and its association with tumour progression",
volume = "32",
number = "6",
pages = "1169-1178",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3456"
}

Milićević, Z. T., Bogojevic, D., Mihailovic, M., Petrović, M. S.,& Krivokapic, Z.. (2008). Molecular characterization of hsp90 isoforms in colorectal cancer cells and its association with tumour progression. in International Journal of Oncology, 32(6), 1169-1178.
https://hdl.handle.net/21.15107/rcub_vinar_3456

Milićević ZT, Bogojevic D, Mihailovic M, Petrović MS, Krivokapic Z. Molecular characterization of hsp90 isoforms in colorectal cancer cells and its association with tumour progression. in International Journal of Oncology. 2008;32(6):1169-1178.
https://hdl.handle.net/21.15107/rcub_vinar_3456 .

Milićević, Zorka T., Bogojevic, D., Mihailovic, M., Petrović, Milica S., Krivokapic, Z., "Molecular characterization of hsp90 isoforms in colorectal cancer cells and its association with tumour progression" in International Journal of Oncology, 32, no. 6 (2008):1169-1178,
https://hdl.handle.net/21.15107/rcub_vinar_3456 .

TY  - CONF
AU  - Milićević, Zorka T.
AU  - Petrovic, MV
AU  - Mihailovic, MV
AU  - Bogojevic, DB
PY  - 2005
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6562
C3  - FEBS Journal
T1  - Cytoplasmic retention of p53 in colorectal cancer cells
VL  - 272
SP  - 127
EP  - 128
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6562
ER  - 

@conference{
author = "Milićević, Zorka T. and Petrovic, MV and Mihailovic, MV and Bogojevic, DB",
year = "2005",
journal = "FEBS Journal",
title = "Cytoplasmic retention of p53 in colorectal cancer cells",
volume = "272",
pages = "127-128",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6562"
}

Milićević, Z. T., Petrovic, M., Mihailovic, M.,& Bogojevic, D.. (2005). Cytoplasmic retention of p53 in colorectal cancer cells. in FEBS Journal, 272, 127-128.
https://hdl.handle.net/21.15107/rcub_vinar_6562

Milićević ZT, Petrovic M, Mihailovic M, Bogojevic D. Cytoplasmic retention of p53 in colorectal cancer cells. in FEBS Journal. 2005;272:127-128.
https://hdl.handle.net/21.15107/rcub_vinar_6562 .

Milićević, Zorka T., Petrovic, MV, Mihailovic, MV, Bogojevic, DB, "Cytoplasmic retention of p53 in colorectal cancer cells" in FEBS Journal, 272 (2005):127-128,
https://hdl.handle.net/21.15107/rcub_vinar_6562 .