TY  - JOUR
AU  - Pinkney, T.
AU  - Gvozdenović, M.
AU  - Jovanović, Dragana J.
AU  - Lausevic, Z.
AU  - Cvetković, D.
AU  - Maravic, M.
AU  - Milovanović, Branislav
AU  - Stojakovic, N.
AU  - Tripkovic, I.
AU  - Mihajlovic, D.
AU  - Nestorović, M.
AU  - Pecic, V.
AU  - Petrović, D.
AU  - Stanojevic, G.
AU  - Barisic, G.
AU  - Dimitrijevic, I.
AU  - Krivokapic, Z.
AU  - Markovic, V.
AU  - Popović, Milan
AU  - Aleksić, A.
AU  - Dabic, D.
AU  - Kostic, I.
AU  - Milojkovic, A.
AU  - Perunicic, V.
AU  - Lukić, Dejan
AU  - Petrović, T.
AU  - Radovanovic, D.
AU  - Radovanovic, Z.
AU  - Cuk, V. M.
AU  - Cuk, V. V.
AU  - Kenic, M.
AU  - Kovacevic, B.
AU  - Krdzic, I.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1667
AB  - Aim The anastomosis technique used following right-sided colonic resection is widely variable and may affect patient outcome. This study aimed to assess the association between leak and anastomosis technique (stapled vs handsewn). Method This was a prospective, multicentre, international audit including patients undergoing elective or emergency right hemicolectomy or ileo-caecal resection operations over a 2-month period in early 2015. The primary outcome measure was the presence of anastomotic leak within 30 days of surgery, determined using a prespecified definition. Mixed effects logistic regression models were used to assess the association between leak and anastomosis method, adjusting for patient, disease and operative cofactors, with centre included as a random-effect variable. Results This study included 3208 patients, of whom 78.4% (n = 2515) underwent surgery for malignancy and 11.7% (n = 375) underwent surgery for Crohns disease. An anastomosis was performed in 94.8% (n = 3041) of patients, which was handsewn in 38.9% (n = 1183) and stapled in 61.1% (n = 1858). Patients undergoing hand-sewn anastomosis were more likely to be emergency admissions (20.5% handsewn vs 12.9% stapled) and to undergo open surgery (54.7% handsewn vs 36.6% stapled). The overall anastomotic leak rate was 8.1% (245/3041), which was similar following handsewn (7.4%) and stapled (8.5%) techniques (P = 0.3). After adjustment for cofactors, the odds of a leak were higher for stapled anastomosis (adjusted OR = 1.43; 95% CI: 1.04-1.95; P = 0.03). Conclusion Despite being used in lower-risk patients, stapled anastomosis was associated with an increased anastomotic leak rate in this observational study. Further research is needed to define patient groups in whom a stapled anastomosis is safe.
T2  - Colorectal Disease
T1  - Relationship between method of anastomosis and anastomotic failure after right hemicolectomy and ileo-caecal resection: an international snapshot audit
VL  - 19
IS  - 8
SP  - O296
EP  - O311
DO  - 10.1111/codi.13646
ER  - 

@article{
author = "Pinkney, T. and Gvozdenović, M. and Jovanović, Dragana J. and Lausevic, Z. and Cvetković, D. and Maravic, M. and Milovanović, Branislav and Stojakovic, N. and Tripkovic, I. and Mihajlovic, D. and Nestorović, M. and Pecic, V. and Petrović, D. and Stanojevic, G. and Barisic, G. and Dimitrijevic, I. and Krivokapic, Z. and Markovic, V. and Popović, Milan and Aleksić, A. and Dabic, D. and Kostic, I. and Milojkovic, A. and Perunicic, V. and Lukić, Dejan and Petrović, T. and Radovanovic, D. and Radovanovic, Z. and Cuk, V. M. and Cuk, V. V. and Kenic, M. and Kovacevic, B. and Krdzic, I.",
year = "2017",
abstract = "Aim The anastomosis technique used following right-sided colonic resection is widely variable and may affect patient outcome. This study aimed to assess the association between leak and anastomosis technique (stapled vs handsewn). Method This was a prospective, multicentre, international audit including patients undergoing elective or emergency right hemicolectomy or ileo-caecal resection operations over a 2-month period in early 2015. The primary outcome measure was the presence of anastomotic leak within 30 days of surgery, determined using a prespecified definition. Mixed effects logistic regression models were used to assess the association between leak and anastomosis method, adjusting for patient, disease and operative cofactors, with centre included as a random-effect variable. Results This study included 3208 patients, of whom 78.4% (n = 2515) underwent surgery for malignancy and 11.7% (n = 375) underwent surgery for Crohns disease. An anastomosis was performed in 94.8% (n = 3041) of patients, which was handsewn in 38.9% (n = 1183) and stapled in 61.1% (n = 1858). Patients undergoing hand-sewn anastomosis were more likely to be emergency admissions (20.5% handsewn vs 12.9% stapled) and to undergo open surgery (54.7% handsewn vs 36.6% stapled). The overall anastomotic leak rate was 8.1% (245/3041), which was similar following handsewn (7.4%) and stapled (8.5%) techniques (P = 0.3). After adjustment for cofactors, the odds of a leak were higher for stapled anastomosis (adjusted OR = 1.43; 95% CI: 1.04-1.95; P = 0.03). Conclusion Despite being used in lower-risk patients, stapled anastomosis was associated with an increased anastomotic leak rate in this observational study. Further research is needed to define patient groups in whom a stapled anastomosis is safe.",
journal = "Colorectal Disease",
title = "Relationship between method of anastomosis and anastomotic failure after right hemicolectomy and ileo-caecal resection: an international snapshot audit",
volume = "19",
number = "8",
pages = "O296-O311",
doi = "10.1111/codi.13646"
}

Pinkney, T., Gvozdenović, M., Jovanović, D. J., Lausevic, Z., Cvetković, D., Maravic, M., Milovanović, B., Stojakovic, N., Tripkovic, I., Mihajlovic, D., Nestorović, M., Pecic, V., Petrović, D., Stanojevic, G., Barisic, G., Dimitrijevic, I., Krivokapic, Z., Markovic, V., Popović, M., Aleksić, A., Dabic, D., Kostic, I., Milojkovic, A., Perunicic, V., Lukić, D., Petrović, T., Radovanovic, D., Radovanovic, Z., Cuk, V. M., Cuk, V. V., Kenic, M., Kovacevic, B.,& Krdzic, I.. (2017). Relationship between method of anastomosis and anastomotic failure after right hemicolectomy and ileo-caecal resection: an international snapshot audit. in Colorectal Disease, 19(8), O296-O311.
https://doi.org/10.1111/codi.13646

Pinkney T, Gvozdenović M, Jovanović DJ, Lausevic Z, Cvetković D, Maravic M, Milovanović B, Stojakovic N, Tripkovic I, Mihajlovic D, Nestorović M, Pecic V, Petrović D, Stanojevic G, Barisic G, Dimitrijevic I, Krivokapic Z, Markovic V, Popović M, Aleksić A, Dabic D, Kostic I, Milojkovic A, Perunicic V, Lukić D, Petrović T, Radovanovic D, Radovanovic Z, Cuk VM, Cuk VV, Kenic M, Kovacevic B, Krdzic I. Relationship between method of anastomosis and anastomotic failure after right hemicolectomy and ileo-caecal resection: an international snapshot audit. in Colorectal Disease. 2017;19(8):O296-O311.
doi:10.1111/codi.13646 .

Pinkney, T., Gvozdenović, M., Jovanović, Dragana J., Lausevic, Z., Cvetković, D., Maravic, M., Milovanović, Branislav, Stojakovic, N., Tripkovic, I., Mihajlovic, D., Nestorović, M., Pecic, V., Petrović, D., Stanojevic, G., Barisic, G., Dimitrijevic, I., Krivokapic, Z., Markovic, V., Popović, Milan, Aleksić, A., Dabic, D., Kostic, I., Milojkovic, A., Perunicic, V., Lukić, Dejan, Petrović, T., Radovanovic, D., Radovanovic, Z., Cuk, V. M., Cuk, V. V., Kenic, M., Kovacevic, B., Krdzic, I., "Relationship between method of anastomosis and anastomotic failure after right hemicolectomy and ileo-caecal resection: an international snapshot audit" in Colorectal Disease, 19, no. 8 (2017):O296-O311,
https://doi.org/10.1111/codi.13646 . .

TY  - JOUR
AU  - Milićević, Zorka T.
AU  - Bogojevic, D.
AU  - Mihailovic, M.
AU  - Petrović, Milica S.
AU  - Krivokapic, Z.
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3456
AB  - A key role of hsp90 in the activity of various oncogenic proteins and pathways is currently of intense interest. To clarify the molecular basis of biological behaviour of colorectal cancers we analysed the expression characteristics of hsp90 in cytosolic, nuclear and plasma membranous fractions of cancer cells. As determined by Western blot assay all hsp90 isoforms studied, a (84 kDa), 8 (86 kDa) and hsp90N (75 kDa), were up-regulated and differentially expressed in various stages of colorectal carcinoma. The inducible hsp90 alpha isoform is a component of invasive phenotype of cancer cells thus pointing to the importance of hsp90 alpha for metastasis generation. The expression of hsp90 beta is definitely higher in poorly-differentiated carcinomas than in well-differentiated cancers, suggesting an involvement of hsp90 beta in the inhibition of cancer cell differentiation. Especially, the expression of cytosolic hsp90N isoform in malignant cells points to the possibility that induction or overexpression of hsp90N might be causally related to tumour formation. Hsp90N is the plasma-membrane-associated protein in poorly-differentiated colorectal cancers with metastasis. This suggests that the expression of hsp90N is elevated with progressive dedifferentiation often associated with advanced cancer stages. Hsp90 was exclusively localized in the invasive front in a majority of metastatic cancers as visualized by immunohistochemical study. Consistent with these facts, the frequent expression of hsp90 alpha and hsp90N on the surface of colorectal cancer cells may enable hsp90 to act as a mediator of metastasis generation. The above results indicate more complex roles for hsp90 in colorectal tumourigenesis. In this way, the hsp90 would be at the crossroads of both signalling and cell migration events.
T2  - International Journal of Oncology
T1  - Molecular characterization of hsp90 isoforms in colorectal cancer cells and its association with tumour progression
VL  - 32
IS  - 6
SP  - 1169
EP  - 1178
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3456
ER  - 

@article{
author = "Milićević, Zorka T. and Bogojevic, D. and Mihailovic, M. and Petrović, Milica S. and Krivokapic, Z.",
year = "2008",
abstract = "A key role of hsp90 in the activity of various oncogenic proteins and pathways is currently of intense interest. To clarify the molecular basis of biological behaviour of colorectal cancers we analysed the expression characteristics of hsp90 in cytosolic, nuclear and plasma membranous fractions of cancer cells. As determined by Western blot assay all hsp90 isoforms studied, a (84 kDa), 8 (86 kDa) and hsp90N (75 kDa), were up-regulated and differentially expressed in various stages of colorectal carcinoma. The inducible hsp90 alpha isoform is a component of invasive phenotype of cancer cells thus pointing to the importance of hsp90 alpha for metastasis generation. The expression of hsp90 beta is definitely higher in poorly-differentiated carcinomas than in well-differentiated cancers, suggesting an involvement of hsp90 beta in the inhibition of cancer cell differentiation. Especially, the expression of cytosolic hsp90N isoform in malignant cells points to the possibility that induction or overexpression of hsp90N might be causally related to tumour formation. Hsp90N is the plasma-membrane-associated protein in poorly-differentiated colorectal cancers with metastasis. This suggests that the expression of hsp90N is elevated with progressive dedifferentiation often associated with advanced cancer stages. Hsp90 was exclusively localized in the invasive front in a majority of metastatic cancers as visualized by immunohistochemical study. Consistent with these facts, the frequent expression of hsp90 alpha and hsp90N on the surface of colorectal cancer cells may enable hsp90 to act as a mediator of metastasis generation. The above results indicate more complex roles for hsp90 in colorectal tumourigenesis. In this way, the hsp90 would be at the crossroads of both signalling and cell migration events.",
journal = "International Journal of Oncology",
title = "Molecular characterization of hsp90 isoforms in colorectal cancer cells and its association with tumour progression",
volume = "32",
number = "6",
pages = "1169-1178",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3456"
}

Milićević, Z. T., Bogojevic, D., Mihailovic, M., Petrović, M. S.,& Krivokapic, Z.. (2008). Molecular characterization of hsp90 isoforms in colorectal cancer cells and its association with tumour progression. in International Journal of Oncology, 32(6), 1169-1178.
https://hdl.handle.net/21.15107/rcub_vinar_3456

Milićević ZT, Bogojevic D, Mihailovic M, Petrović MS, Krivokapic Z. Molecular characterization of hsp90 isoforms in colorectal cancer cells and its association with tumour progression. in International Journal of Oncology. 2008;32(6):1169-1178.
https://hdl.handle.net/21.15107/rcub_vinar_3456 .

Milićević, Zorka T., Bogojevic, D., Mihailovic, M., Petrović, Milica S., Krivokapic, Z., "Molecular characterization of hsp90 isoforms in colorectal cancer cells and its association with tumour progression" in International Journal of Oncology, 32, no. 6 (2008):1169-1178,
https://hdl.handle.net/21.15107/rcub_vinar_3456 .