TY  - JOUR
AU  - Popović, Milan
AU  - Paskas, Svetlana
AU  - Živković, Maja
AU  - Burysek, Ladislav
AU  - Laumonnier, Yves
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4059
AB  - Human cytomegalovirus (HCMV) establishes a life-long persistent infection. HCMV infection could be associated with chronic inflammatory diseases, such as cardiovascular disease and atherosclerosis. Here we observed that in HCMV (AD-169) pre-exposed human umbilical vein endothelial cells (HUVEC), thrombin-induced expression of IL-1 alpha and M-CSF is markedly enhanced compared to the un-exposed cells. Study of the expression of thrombin receptor genes in HUVEC showed that HCMV triggered a time- and concentration-dependent expression of the thrombin receptors PAR1, PAR3 and PAR4 at the mRNA level. Induction of PAR1 and PAR3 mRNA expression is due to transcriptional activation of their promoters as shown by gene reporter assay. Furthermore, the virus induced expression of PAR1 and PAR3 but not PAR4 proteins, as analyzed by Western immunoblotting. However, flow cytometric analysis revealed that only PAR3, expressed at very low level in control HUVEC, is induced at the surface during the exposure to the virus. Our data suggest that although exposure to HCMV induces a minor increase of cell-surface receptors expression, it does make endothelial cells more responsive to additional thrombin stimulation.
T2  - Journal of Thrombosis and Thrombolysis
T1  - Human cytomegalovirus increases HUVEC sensitivity to thrombin and modulates expression of thrombin receptors
VL  - 30
IS  - 2
SP  - 164
EP  - 171
DO  - 10.1007/s11239-010-0447-7
ER  - 

@article{
author = "Popović, Milan and Paskas, Svetlana and Živković, Maja and Burysek, Ladislav and Laumonnier, Yves",
year = "2010",
abstract = "Human cytomegalovirus (HCMV) establishes a life-long persistent infection. HCMV infection could be associated with chronic inflammatory diseases, such as cardiovascular disease and atherosclerosis. Here we observed that in HCMV (AD-169) pre-exposed human umbilical vein endothelial cells (HUVEC), thrombin-induced expression of IL-1 alpha and M-CSF is markedly enhanced compared to the un-exposed cells. Study of the expression of thrombin receptor genes in HUVEC showed that HCMV triggered a time- and concentration-dependent expression of the thrombin receptors PAR1, PAR3 and PAR4 at the mRNA level. Induction of PAR1 and PAR3 mRNA expression is due to transcriptional activation of their promoters as shown by gene reporter assay. Furthermore, the virus induced expression of PAR1 and PAR3 but not PAR4 proteins, as analyzed by Western immunoblotting. However, flow cytometric analysis revealed that only PAR3, expressed at very low level in control HUVEC, is induced at the surface during the exposure to the virus. Our data suggest that although exposure to HCMV induces a minor increase of cell-surface receptors expression, it does make endothelial cells more responsive to additional thrombin stimulation.",
journal = "Journal of Thrombosis and Thrombolysis",
title = "Human cytomegalovirus increases HUVEC sensitivity to thrombin and modulates expression of thrombin receptors",
volume = "30",
number = "2",
pages = "164-171",
doi = "10.1007/s11239-010-0447-7"
}

Popović, M., Paskas, S., Živković, M., Burysek, L.,& Laumonnier, Y.. (2010). Human cytomegalovirus increases HUVEC sensitivity to thrombin and modulates expression of thrombin receptors. in Journal of Thrombosis and Thrombolysis, 30(2), 164-171.
https://doi.org/10.1007/s11239-010-0447-7

Popović M, Paskas S, Živković M, Burysek L, Laumonnier Y. Human cytomegalovirus increases HUVEC sensitivity to thrombin and modulates expression of thrombin receptors. in Journal of Thrombosis and Thrombolysis. 2010;30(2):164-171.
doi:10.1007/s11239-010-0447-7 .

Popović, Milan, Paskas, Svetlana, Živković, Maja, Burysek, Ladislav, Laumonnier, Yves, "Human cytomegalovirus increases HUVEC sensitivity to thrombin and modulates expression of thrombin receptors" in Journal of Thrombosis and Thrombolysis, 30, no. 2 (2010):164-171,
https://doi.org/10.1007/s11239-010-0447-7 . .

TY  - JOUR
AU  - Popović, Milan
AU  - Paskas, Svetlana
AU  - Živković, Maja
AU  - Đurić-Delić, Tamara
AU  - Stanković, Aleksandra
AU  - Burysek, L.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3864
AB  - We studied the effect of HCMV (AD-169) on the regulation of PAR1 reporter activity in HUVEC. In HUVEC, HCMV significantly induced activation of PAR1 reporter constructs carrying AP-2-like and two complexes of AP-2/Sp1-binding elements. Shorter constructs with no AP-2-like binding elements had lower activity upon virus inoculation. Moreover, activity of the shortest construct without binding elements was not significantly increased in virus-exposed cells. Furthermore, in HCMV-exposed cells, activity of the PARI reporter was significantly augmented upon p52/RelB transfection. Taken together, the data indicate that activity of the PAR1 promoter is highly dependent on AP-2/Sp1 binding elements and mediated by p52/RelB regulatory factors.
T2  - Archives of Biological Sciences
T1  - In Hcmv-Exposed Huvec, P52/Relb Regulatory Factors Mediate Activation of the Human Par1 Gene Promoter
VL  - 61
IS  - 4
SP  - 613
EP  - 618
DO  - 10.2298/ABS0904613P
ER  - 

@article{
author = "Popović, Milan and Paskas, Svetlana and Živković, Maja and Đurić-Delić, Tamara and Stanković, Aleksandra and Burysek, L.",
year = "2009",
abstract = "We studied the effect of HCMV (AD-169) on the regulation of PAR1 reporter activity in HUVEC. In HUVEC, HCMV significantly induced activation of PAR1 reporter constructs carrying AP-2-like and two complexes of AP-2/Sp1-binding elements. Shorter constructs with no AP-2-like binding elements had lower activity upon virus inoculation. Moreover, activity of the shortest construct without binding elements was not significantly increased in virus-exposed cells. Furthermore, in HCMV-exposed cells, activity of the PARI reporter was significantly augmented upon p52/RelB transfection. Taken together, the data indicate that activity of the PAR1 promoter is highly dependent on AP-2/Sp1 binding elements and mediated by p52/RelB regulatory factors.",
journal = "Archives of Biological Sciences",
title = "In Hcmv-Exposed Huvec, P52/Relb Regulatory Factors Mediate Activation of the Human Par1 Gene Promoter",
volume = "61",
number = "4",
pages = "613-618",
doi = "10.2298/ABS0904613P"
}

Popović, M., Paskas, S., Živković, M., Đurić-Delić, T., Stanković, A.,& Burysek, L.. (2009). In Hcmv-Exposed Huvec, P52/Relb Regulatory Factors Mediate Activation of the Human Par1 Gene Promoter. in Archives of Biological Sciences, 61(4), 613-618.
https://doi.org/10.2298/ABS0904613P

Popović M, Paskas S, Živković M, Đurić-Delić T, Stanković A, Burysek L. In Hcmv-Exposed Huvec, P52/Relb Regulatory Factors Mediate Activation of the Human Par1 Gene Promoter. in Archives of Biological Sciences. 2009;61(4):613-618.
doi:10.2298/ABS0904613P .

Popović, Milan, Paskas, Svetlana, Živković, Maja, Đurić-Delić, Tamara, Stanković, Aleksandra, Burysek, L., "In Hcmv-Exposed Huvec, P52/Relb Regulatory Factors Mediate Activation of the Human Par1 Gene Promoter" in Archives of Biological Sciences, 61, no. 4 (2009):613-618,
https://doi.org/10.2298/ABS0904613P . .