TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Obradović, Milan M.
AU  - Sudar-Milovanović, Emina
AU  - Stewart, Alan J.
AU  - Pitt, Samantha J.
AU  - Alavantić, Dragan
AU  - Aleksić, Ema
AU  - Isenović, Esma R.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1812
AB  - The aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na+/K+-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150-200 g) were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. The results show that rats fed a HF diet exhibited a decrease in phosphorylation of the alpha(1) subunit of Na+/K+-ATPase by 30% (p LT 0.05), expression of total alpha(1) subunit of Na+/K+-ATPase by 31% (p LT 0.05), and association of IRS1 with p85 subunit of PI3K by 42% (p LT 0.05), while the levels of cardiac RhoA and ROCK2 were significantly increased by 84% (p LT 0.01) and 62% (p LT 0.05), respectively. Our results suggest that a HF diet alters cardiac Na+/K+-ATPase expression via molecular mechanisms involving RhoA/ROCK and IRS-1/PI3K signalling in female rats.
T2  - Molecular and Cellular Biochemistry
T1  - Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet
VL  - 436
IS  - 1-2
SP  - 49
EP  - 58
DO  - 10.1007/s11010-017-3077-y
ER  - 

@article{
author = "Jovanović, Aleksandra and Obradović, Milan M. and Sudar-Milovanović, Emina and Stewart, Alan J. and Pitt, Samantha J. and Alavantić, Dragan and Aleksić, Ema and Isenović, Esma R.",
year = "2017",
abstract = "The aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na+/K+-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150-200 g) were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. The results show that rats fed a HF diet exhibited a decrease in phosphorylation of the alpha(1) subunit of Na+/K+-ATPase by 30% (p LT 0.05), expression of total alpha(1) subunit of Na+/K+-ATPase by 31% (p LT 0.05), and association of IRS1 with p85 subunit of PI3K by 42% (p LT 0.05), while the levels of cardiac RhoA and ROCK2 were significantly increased by 84% (p LT 0.01) and 62% (p LT 0.05), respectively. Our results suggest that a HF diet alters cardiac Na+/K+-ATPase expression via molecular mechanisms involving RhoA/ROCK and IRS-1/PI3K signalling in female rats.",
journal = "Molecular and Cellular Biochemistry",
title = "Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet",
volume = "436",
number = "1-2",
pages = "49-58",
doi = "10.1007/s11010-017-3077-y"
}

Jovanović, A., Obradović, M. M., Sudar-Milovanović, E., Stewart, A. J., Pitt, S. J., Alavantić, D., Aleksić, E.,& Isenović, E. R.. (2017). Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet. in Molecular and Cellular Biochemistry, 436(1-2), 49-58.
https://doi.org/10.1007/s11010-017-3077-y

Jovanović A, Obradović MM, Sudar-Milovanović E, Stewart AJ, Pitt SJ, Alavantić D, Aleksić E, Isenović ER. Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet. in Molecular and Cellular Biochemistry. 2017;436(1-2):49-58.
doi:10.1007/s11010-017-3077-y .

Jovanović, Aleksandra, Obradović, Milan M., Sudar-Milovanović, Emina, Stewart, Alan J., Pitt, Samantha J., Alavantić, Dragan, Aleksić, Ema, Isenović, Esma R., "Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet" in Molecular and Cellular Biochemistry, 436, no. 1-2 (2017):49-58,
https://doi.org/10.1007/s11010-017-3077-y . .