TY  - JOUR
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka
AU  - Ajdžanovic, Vladimir
AU  - Živanović, Jasmina
AU  - Isenović, Esma R.
AU  - Popovska-Percinic, Florina
AU  - Milošević, Verica
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/535
AB  - Thyroid C-cells produce calcitonin (CT), a hypocalcemic hormone, that acts as an inhibitor of bone resorption. In this study, we investigated the effects of tamoxifen (TAM) as a selective estrogen receptor modulator on thyroid C-cells, trabecular bone and biochemical markers of bone metabolism in an animal model of androgen deficiency, represented by middle-aged orchidectomized (Orx) rats. Fifteen-month-old male Wistar rats were divided into: Orx and sham-operated (SO) groups. Rats from one Orx group were injected subcutaneously with TAM citrate (Orx + TAM; 0.3 mgkg(-1) b.w.), while the rats from SO and a second Orx group received vehicle alone, once a day for 3 weeks. The peroxidase-antiperoxidase method was applied for localization of CT in C-cells. Thyroid C-cells were morphometrically and ultrastructurally analyzed. An ImageJ image-processing program was used to measure bone histomorphometric parameters. Blood serum samples were analyzed for CT, osteocalcin (OC), calcium (Ca2+) and phosphorus (P). Urinary Ca2+ concentrations were measured. TAM treatment significantly increased thyroid C-cell volume (V-c) and serum CT when compared with vehicle-treated Orx rats. Analysis of trabecular microarchitecture of the tibia showed that administration of TAM significantly increased cancellous bone area, trabecular thickness and trabecular number, whereas trabecular separation was significantly decreased compared with vehicle-treated Orx rats. Serum OC and urinary Ca2+ concentrations were significantly lower in comparison with the control Orx group. These results indicate that in our rat model of androgen deficiency, TAM stimulated calcitonin-producing thyroid C-cells and increased trabecular bone mass.
T2  - Journal of Anatomy
T1  - Tamoxifen stimulates calcitonin-producing thyroid C-cells and prevents trabecular bone loss in a rat model of androgen deficiency
VL  - 226
IS  - 5
SP  - 489
EP  - 496
DO  - 10.1111/joa.12298
ER  - 

@article{
author = "Filipović, Branko and Šošić-Jurjević, Branka and Ajdžanovic, Vladimir and Živanović, Jasmina and Isenović, Esma R. and Popovska-Percinic, Florina and Milošević, Verica",
year = "2015",
abstract = "Thyroid C-cells produce calcitonin (CT), a hypocalcemic hormone, that acts as an inhibitor of bone resorption. In this study, we investigated the effects of tamoxifen (TAM) as a selective estrogen receptor modulator on thyroid C-cells, trabecular bone and biochemical markers of bone metabolism in an animal model of androgen deficiency, represented by middle-aged orchidectomized (Orx) rats. Fifteen-month-old male Wistar rats were divided into: Orx and sham-operated (SO) groups. Rats from one Orx group were injected subcutaneously with TAM citrate (Orx + TAM; 0.3 mgkg(-1) b.w.), while the rats from SO and a second Orx group received vehicle alone, once a day for 3 weeks. The peroxidase-antiperoxidase method was applied for localization of CT in C-cells. Thyroid C-cells were morphometrically and ultrastructurally analyzed. An ImageJ image-processing program was used to measure bone histomorphometric parameters. Blood serum samples were analyzed for CT, osteocalcin (OC), calcium (Ca2+) and phosphorus (P). Urinary Ca2+ concentrations were measured. TAM treatment significantly increased thyroid C-cell volume (V-c) and serum CT when compared with vehicle-treated Orx rats. Analysis of trabecular microarchitecture of the tibia showed that administration of TAM significantly increased cancellous bone area, trabecular thickness and trabecular number, whereas trabecular separation was significantly decreased compared with vehicle-treated Orx rats. Serum OC and urinary Ca2+ concentrations were significantly lower in comparison with the control Orx group. These results indicate that in our rat model of androgen deficiency, TAM stimulated calcitonin-producing thyroid C-cells and increased trabecular bone mass.",
journal = "Journal of Anatomy",
title = "Tamoxifen stimulates calcitonin-producing thyroid C-cells and prevents trabecular bone loss in a rat model of androgen deficiency",
volume = "226",
number = "5",
pages = "489-496",
doi = "10.1111/joa.12298"
}

Filipović, B., Šošić-Jurjević, B., Ajdžanovic, V., Živanović, J., Isenović, E. R., Popovska-Percinic, F.,& Milošević, V.. (2015). Tamoxifen stimulates calcitonin-producing thyroid C-cells and prevents trabecular bone loss in a rat model of androgen deficiency. in Journal of Anatomy, 226(5), 489-496.
https://doi.org/10.1111/joa.12298

Filipović B, Šošić-Jurjević B, Ajdžanovic V, Živanović J, Isenović ER, Popovska-Percinic F, Milošević V. Tamoxifen stimulates calcitonin-producing thyroid C-cells and prevents trabecular bone loss in a rat model of androgen deficiency. in Journal of Anatomy. 2015;226(5):489-496.
doi:10.1111/joa.12298 .

Filipović, Branko, Šošić-Jurjević, Branka, Ajdžanovic, Vladimir, Živanović, Jasmina, Isenović, Esma R., Popovska-Percinic, Florina, Milošević, Verica, "Tamoxifen stimulates calcitonin-producing thyroid C-cells and prevents trabecular bone loss in a rat model of androgen deficiency" in Journal of Anatomy, 226, no. 5 (2015):489-496,
https://doi.org/10.1111/joa.12298 . .