TY  - JOUR
AU  - Vranić-Mandušić, Vesna
AU  - Subota, Vesna
AU  - Savovski, K
AU  - Medic, L
AU  - Dramićanin, Tatjana
AU  - Jozanov-Stankov, Olga
AU  - Popov-Celeketic, D
AU  - Jokanović, Milan
AU  - Dimitrijević, Bogomir B.
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2701
AB  - Tiazofurin (TZF) is a cytostatic drug that leads to depletion of the GTP pool in tumor and normal cells via its active metabolite tiazofurin-adenine dinucleotide (TAD). TAD was detected in different cell lines, but not in erythrocytes, so the mechanism of erythrocytotoxicity of TZF remains unclear. The purpose of this study was to evaluate in vitro and in vivo action of tiazofurin on rat erythrocytes (RBC). After two decades of clinical trials the question of erythrocytotoxicity of TZF had remained unexplained making this study justified. Since we have previously demonstrated early erythrocytotoxic effects in male Wistar rats, we extend this finding on isolated RBC. Isolated erythrocytes from untreated animals were treated in buffered solution or plasma containing TZF In addition, groups of 10 rats were treated with 200 and 1000 mg/kg of TZF and hematologic parameters were analyzed by flowcytometry and by the analysis of the peripheral blood smears. Early signs of hemolysis or aberrant structures were monitored by scanning probe microscopy (SPM). We suggest that correlation exists between early erythrocytotoxicity and irregularities in erythrocyte morphology and membrane integrity. We also found that TZF affects responsiveness to oxidative stress. This is in concordance with flowcytometric findings describing anisocytosis and anisochromosis of RBC. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
T2  - Toxicology Letters
T1  - Erythrocytotoxicity of tiazofurin in vivo and in vitro detected by scanning probe microscopy
VL  - 146
IS  - 3
SP  - 275
EP  - 284
DO  - 10.1016/j.toxlet.2003.10.013
ER  - 

@article{
author = "Vranić-Mandušić, Vesna and Subota, Vesna and Savovski, K and Medic, L and Dramićanin, Tatjana and Jozanov-Stankov, Olga and Popov-Celeketic, D and Jokanović, Milan and Dimitrijević, Bogomir B.",
year = "2004",
abstract = "Tiazofurin (TZF) is a cytostatic drug that leads to depletion of the GTP pool in tumor and normal cells via its active metabolite tiazofurin-adenine dinucleotide (TAD). TAD was detected in different cell lines, but not in erythrocytes, so the mechanism of erythrocytotoxicity of TZF remains unclear. The purpose of this study was to evaluate in vitro and in vivo action of tiazofurin on rat erythrocytes (RBC). After two decades of clinical trials the question of erythrocytotoxicity of TZF had remained unexplained making this study justified. Since we have previously demonstrated early erythrocytotoxic effects in male Wistar rats, we extend this finding on isolated RBC. Isolated erythrocytes from untreated animals were treated in buffered solution or plasma containing TZF In addition, groups of 10 rats were treated with 200 and 1000 mg/kg of TZF and hematologic parameters were analyzed by flowcytometry and by the analysis of the peripheral blood smears. Early signs of hemolysis or aberrant structures were monitored by scanning probe microscopy (SPM). We suggest that correlation exists between early erythrocytotoxicity and irregularities in erythrocyte morphology and membrane integrity. We also found that TZF affects responsiveness to oxidative stress. This is in concordance with flowcytometric findings describing anisocytosis and anisochromosis of RBC. (C) 2003 Elsevier Ireland Ltd. All rights reserved.",
journal = "Toxicology Letters",
title = "Erythrocytotoxicity of tiazofurin in vivo and in vitro detected by scanning probe microscopy",
volume = "146",
number = "3",
pages = "275-284",
doi = "10.1016/j.toxlet.2003.10.013"
}

Vranić-Mandušić, V., Subota, V., Savovski, K., Medic, L., Dramićanin, T., Jozanov-Stankov, O., Popov-Celeketic, D., Jokanović, M.,& Dimitrijević, B. B.. (2004). Erythrocytotoxicity of tiazofurin in vivo and in vitro detected by scanning probe microscopy. in Toxicology Letters, 146(3), 275-284.
https://doi.org/10.1016/j.toxlet.2003.10.013

Vranić-Mandušić V, Subota V, Savovski K, Medic L, Dramićanin T, Jozanov-Stankov O, Popov-Celeketic D, Jokanović M, Dimitrijević BB. Erythrocytotoxicity of tiazofurin in vivo and in vitro detected by scanning probe microscopy. in Toxicology Letters. 2004;146(3):275-284.
doi:10.1016/j.toxlet.2003.10.013 .

Vranić-Mandušić, Vesna, Subota, Vesna, Savovski, K, Medic, L, Dramićanin, Tatjana, Jozanov-Stankov, Olga, Popov-Celeketic, D, Jokanović, Milan, Dimitrijević, Bogomir B., "Erythrocytotoxicity of tiazofurin in vivo and in vitro detected by scanning probe microscopy" in Toxicology Letters, 146, no. 3 (2004):275-284,
https://doi.org/10.1016/j.toxlet.2003.10.013 . .