TY  - JOUR
AU  - Dorontić, Slađana
AU  - Bonasera, Aurelio
AU  - Scopelliti, Michelangelo
AU  - Marković, Olivera
AU  - Verbić, Tatjana
AU  - Sredojević, Dušan
AU  - Ciasca, Gabriele
AU  - Di Santo, Riccardo
AU  - Mead, James L.
AU  - Budimir, Milica
AU  - Bajuk-Bogdanović, Danica
AU  - Mojsin, Marija
AU  - Pejić, Jelena
AU  - Stevanović, Milena
AU  - Jovanović, Svetlana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13322
AB  - The widespread usage of the herbicide glyphosate calls for urgent action, aiming at the development of new, simple, low-cost, and eco-friendly detection approaches. In the last decade, investigation of graphene quantum dots (GQDs) as potential optical probes for various pollutants rapidly grew, thanks to their easy-manipulative structure, remarkable photoluminescence (PL) in the visible part of the spectrum, good dispersibility, biocompatibility, and non-toxicity, as well. Herein, a fast, simple, and environmentally friendly method for GQDs structural modification is presented. GQDs raw powder was exposed to γ- rays at three different doses (100, 200, and 300 kGy) in air, without any solvent or reagents. Irradiation of dots under such affordable conditions led to the additional incorporation of oxygen-containing moieties in the GQD structure. For the first time, oxygen-rich GQDs irradiated at a 300 kGy dose were successfully applied as direct turn-off PL probe for glyphosate detection. The high coefficient of determination (R-squared (R2) = 0.99) and very low limit of detection (3.02 μmol L-1) reveal good linearity between the potential sensor and analyte, as well as sensitivity. Glyphosate was successfully detected in celery samples, with a recovery value of 107 ± 0.85%. To evaluate the biological safety of the proposed sensing probe, [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) and the hemolysis assays were performed. Obtained results show that irradiated and non-irradiated GQDs did not cause the death of MRC-5 cells, and hemolysis of erythrocytes. The obtained results demonstrate that GQDs irradiated in an air medium can be potentially applied for glyphosate detection.
T2  - Journal of Environmental Chemical Engineering
T1  - High-performing structural optimization of graphene quantum dots as glyphosate herbicide photoluminescent probes: real case studies and mechanism insights
VL  - 12
IS  - 4
SP  - 113193
DO  - 10.1016/j.jece.2024.113193
ER  - 

@article{
author = "Dorontić, Slađana and Bonasera, Aurelio and Scopelliti, Michelangelo and Marković, Olivera and Verbić, Tatjana and Sredojević, Dušan and Ciasca, Gabriele and Di Santo, Riccardo and Mead, James L. and Budimir, Milica and Bajuk-Bogdanović, Danica and Mojsin, Marija and Pejić, Jelena and Stevanović, Milena and Jovanović, Svetlana",
year = "2024",
abstract = "The widespread usage of the herbicide glyphosate calls for urgent action, aiming at the development of new, simple, low-cost, and eco-friendly detection approaches. In the last decade, investigation of graphene quantum dots (GQDs) as potential optical probes for various pollutants rapidly grew, thanks to their easy-manipulative structure, remarkable photoluminescence (PL) in the visible part of the spectrum, good dispersibility, biocompatibility, and non-toxicity, as well. Herein, a fast, simple, and environmentally friendly method for GQDs structural modification is presented. GQDs raw powder was exposed to γ- rays at three different doses (100, 200, and 300 kGy) in air, without any solvent or reagents. Irradiation of dots under such affordable conditions led to the additional incorporation of oxygen-containing moieties in the GQD structure. For the first time, oxygen-rich GQDs irradiated at a 300 kGy dose were successfully applied as direct turn-off PL probe for glyphosate detection. The high coefficient of determination (R-squared (R2) = 0.99) and very low limit of detection (3.02 μmol L-1) reveal good linearity between the potential sensor and analyte, as well as sensitivity. Glyphosate was successfully detected in celery samples, with a recovery value of 107 ± 0.85%. To evaluate the biological safety of the proposed sensing probe, [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) and the hemolysis assays were performed. Obtained results show that irradiated and non-irradiated GQDs did not cause the death of MRC-5 cells, and hemolysis of erythrocytes. The obtained results demonstrate that GQDs irradiated in an air medium can be potentially applied for glyphosate detection.",
journal = "Journal of Environmental Chemical Engineering",
title = "High-performing structural optimization of graphene quantum dots as glyphosate herbicide photoluminescent probes: real case studies and mechanism insights",
volume = "12",
number = "4",
pages = "113193",
doi = "10.1016/j.jece.2024.113193"
}

Dorontić, S., Bonasera, A., Scopelliti, M., Marković, O., Verbić, T., Sredojević, D., Ciasca, G., Di Santo, R., Mead, J. L., Budimir, M., Bajuk-Bogdanović, D., Mojsin, M., Pejić, J., Stevanović, M.,& Jovanović, S.. (2024). High-performing structural optimization of graphene quantum dots as glyphosate herbicide photoluminescent probes: real case studies and mechanism insights. in Journal of Environmental Chemical Engineering, 12(4), 113193.
https://doi.org/10.1016/j.jece.2024.113193

Dorontić S, Bonasera A, Scopelliti M, Marković O, Verbić T, Sredojević D, Ciasca G, Di Santo R, Mead JL, Budimir M, Bajuk-Bogdanović D, Mojsin M, Pejić J, Stevanović M, Jovanović S. High-performing structural optimization of graphene quantum dots as glyphosate herbicide photoluminescent probes: real case studies and mechanism insights. in Journal of Environmental Chemical Engineering. 2024;12(4):113193.
doi:10.1016/j.jece.2024.113193 .

Dorontić, Slađana, Bonasera, Aurelio, Scopelliti, Michelangelo, Marković, Olivera, Verbić, Tatjana, Sredojević, Dušan, Ciasca, Gabriele, Di Santo, Riccardo, Mead, James L., Budimir, Milica, Bajuk-Bogdanović, Danica, Mojsin, Marija, Pejić, Jelena, Stevanović, Milena, Jovanović, Svetlana, "High-performing structural optimization of graphene quantum dots as glyphosate herbicide photoluminescent probes: real case studies and mechanism insights" in Journal of Environmental Chemical Engineering, 12, no. 4 (2024):113193,
https://doi.org/10.1016/j.jece.2024.113193 . .

TY  - JOUR
AU  - Di Santo, Riccardo
AU  - Romanò, Sabrina
AU  - Mazzini, Alberto
AU  - Jovanović, Svetlana P.
AU  - Nocca, Giuseppina
AU  - Campi, Gaetano
AU  - Papi, Massimiliano
AU  - De Spirito, Marco
AU  - Di Giacinto, Flavio
AU  - Ciasca, Gabriele
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9832
AB  - Exosomes (EXOs) are nano-sized vesicles secreted by most cell types. They are abundant in bio-fluids and harbor specific molecular constituents from their parental cells. Due to these characteristics, EXOs have a great potential in cancer diagnostics for liquid biopsy and personalized medicine. Despite this unique potential, EXOs are not yet widely applied in clinical settings, with two main factors hindering their translational process in diagnostics. Firstly, conventional extraction methods are time-consuming, require large sample volumes and expensive equipment, and often do not provide high-purity samples. Secondly, characterization methods have some limitations, because they are often qualitative, need extensive labeling or complex sampling procedures that can induce artifacts. In this context, novel label-free approaches are rapidly emerging, and are holding potential to revolutionize EXO diagnostics. These methods include the use of nanodevices for EXO purification, and vibrational spectroscopies, scattering, and nanoindentation for characterization. In this progress report, we summarize recent key advances in label-free techniques for EXO purification and characterization. We point out that these methods contribute to reducing costs and processing times, provide complementary information compared to the conventional characterization techniques, and enhance flexibility, thus favoring the discovery of novel and unexplored EXO-based biomarkers. In this process, the impact of nanotechnology is systematically highlighted, showing how the effectiveness of these techniques can be enhanced using nanomaterials, such as plasmonic nanoparticles and nanostructured surfaces, which enable the exploitation of advanced physical phenomena occurring at the nanoscale level.
T2  - Nanomaterials
T1  - Recent advances in the label-free characterization of exosomes for cancer liquid biopsy: From scattering and spectroscopy to nanoindentation and nanodevices
VL  - 11
IS  - 6
SP  - 1476
DO  - 10.3390/nano11061476
ER  - 

@article{
author = "Di Santo, Riccardo and Romanò, Sabrina and Mazzini, Alberto and Jovanović, Svetlana P. and Nocca, Giuseppina and Campi, Gaetano and Papi, Massimiliano and De Spirito, Marco and Di Giacinto, Flavio and Ciasca, Gabriele",
year = "2021",
abstract = "Exosomes (EXOs) are nano-sized vesicles secreted by most cell types. They are abundant in bio-fluids and harbor specific molecular constituents from their parental cells. Due to these characteristics, EXOs have a great potential in cancer diagnostics for liquid biopsy and personalized medicine. Despite this unique potential, EXOs are not yet widely applied in clinical settings, with two main factors hindering their translational process in diagnostics. Firstly, conventional extraction methods are time-consuming, require large sample volumes and expensive equipment, and often do not provide high-purity samples. Secondly, characterization methods have some limitations, because they are often qualitative, need extensive labeling or complex sampling procedures that can induce artifacts. In this context, novel label-free approaches are rapidly emerging, and are holding potential to revolutionize EXO diagnostics. These methods include the use of nanodevices for EXO purification, and vibrational spectroscopies, scattering, and nanoindentation for characterization. In this progress report, we summarize recent key advances in label-free techniques for EXO purification and characterization. We point out that these methods contribute to reducing costs and processing times, provide complementary information compared to the conventional characterization techniques, and enhance flexibility, thus favoring the discovery of novel and unexplored EXO-based biomarkers. In this process, the impact of nanotechnology is systematically highlighted, showing how the effectiveness of these techniques can be enhanced using nanomaterials, such as plasmonic nanoparticles and nanostructured surfaces, which enable the exploitation of advanced physical phenomena occurring at the nanoscale level.",
journal = "Nanomaterials",
title = "Recent advances in the label-free characterization of exosomes for cancer liquid biopsy: From scattering and spectroscopy to nanoindentation and nanodevices",
volume = "11",
number = "6",
pages = "1476",
doi = "10.3390/nano11061476"
}

Di Santo, R., Romanò, S., Mazzini, A., Jovanović, S. P., Nocca, G., Campi, G., Papi, M., De Spirito, M., Di Giacinto, F.,& Ciasca, G.. (2021). Recent advances in the label-free characterization of exosomes for cancer liquid biopsy: From scattering and spectroscopy to nanoindentation and nanodevices. in Nanomaterials, 11(6), 1476.
https://doi.org/10.3390/nano11061476

Di Santo R, Romanò S, Mazzini A, Jovanović SP, Nocca G, Campi G, Papi M, De Spirito M, Di Giacinto F, Ciasca G. Recent advances in the label-free characterization of exosomes for cancer liquid biopsy: From scattering and spectroscopy to nanoindentation and nanodevices. in Nanomaterials. 2021;11(6):1476.
doi:10.3390/nano11061476 .

Di Santo, Riccardo, Romanò, Sabrina, Mazzini, Alberto, Jovanović, Svetlana P., Nocca, Giuseppina, Campi, Gaetano, Papi, Massimiliano, De Spirito, Marco, Di Giacinto, Flavio, Ciasca, Gabriele, "Recent advances in the label-free characterization of exosomes for cancer liquid biopsy: From scattering and spectroscopy to nanoindentation and nanodevices" in Nanomaterials, 11, no. 6 (2021):1476,
https://doi.org/10.3390/nano11061476 . .