TY  - JOUR
AU  - Velebit, Jelena
AU  - Kovacic, Petra Brina
AU  - Prebil, Mateja
AU  - Chowdhury, Helena H.
AU  - Grilc, Sonja
AU  - Kreft, Marko
AU  - Jensen, Jorgen
AU  - Isenović, Esma R.
AU  - Zorec, Robert
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3537
AB  - In this study we hypothesized that rosiglitazone, an antidiabetic high-affinity agonist for the peroxisome proliferator-activated receptor gamma, affects the plasma membrane (PM) turnover in single 3T3-L1 adipocytes. To study the PM turnover, the patch-clamp electrophysiological method was used to measure changes in membrane capacitance (C-m), a parameter linearly related to the PM area. Microscopy results show that the presence of rosiglitazone in the differentiating medium significantly increased the differentiation of 3T3-L1 adipocytes in cell culture, based on oil red O-stained area (11.4 +/- 1.2%) vs. controls (3.1 +/- 0.5%). Moreover, rosiglitazone treatment significantly reduced the size of single 3T3-L1 adipocytes; their average radius of 21.1 +/- 1.1 mu m in controls was reduced to 17.5 +/- 0.5 mu m in rosiglitazone-treated cells. Consistent with this, insulin application increased the rate of C-m increase to 2.34 +/- 0.10%/min, which was significantly different from controls (0.12 +/- 0.08%/min). However, pretreatment of cells with rosiglitazone prior to the treatment with insulin resulted in an attenuated rate of C (m) increase. These data support the involvement of insulin in the modulation of membrane area and show that treatment by rosiglitazone reduced the insulin-mediated membrane area increase in 3T3-L1 adipocytes.
T2  - Journal of Membrane Biology
T1  - Rosiglitazone modulates insulin-induced plasma membrane area changes in single 3T3-L1 adipocytes
VL  - 223
IS  - 3
SP  - 141
EP  - 149
DO  - 10.1007/s00232-008-9120-x
ER  - 

@article{
author = "Velebit, Jelena and Kovacic, Petra Brina and Prebil, Mateja and Chowdhury, Helena H. and Grilc, Sonja and Kreft, Marko and Jensen, Jorgen and Isenović, Esma R. and Zorec, Robert",
year = "2008",
abstract = "In this study we hypothesized that rosiglitazone, an antidiabetic high-affinity agonist for the peroxisome proliferator-activated receptor gamma, affects the plasma membrane (PM) turnover in single 3T3-L1 adipocytes. To study the PM turnover, the patch-clamp electrophysiological method was used to measure changes in membrane capacitance (C-m), a parameter linearly related to the PM area. Microscopy results show that the presence of rosiglitazone in the differentiating medium significantly increased the differentiation of 3T3-L1 adipocytes in cell culture, based on oil red O-stained area (11.4 +/- 1.2%) vs. controls (3.1 +/- 0.5%). Moreover, rosiglitazone treatment significantly reduced the size of single 3T3-L1 adipocytes; their average radius of 21.1 +/- 1.1 mu m in controls was reduced to 17.5 +/- 0.5 mu m in rosiglitazone-treated cells. Consistent with this, insulin application increased the rate of C-m increase to 2.34 +/- 0.10%/min, which was significantly different from controls (0.12 +/- 0.08%/min). However, pretreatment of cells with rosiglitazone prior to the treatment with insulin resulted in an attenuated rate of C (m) increase. These data support the involvement of insulin in the modulation of membrane area and show that treatment by rosiglitazone reduced the insulin-mediated membrane area increase in 3T3-L1 adipocytes.",
journal = "Journal of Membrane Biology",
title = "Rosiglitazone modulates insulin-induced plasma membrane area changes in single 3T3-L1 adipocytes",
volume = "223",
number = "3",
pages = "141-149",
doi = "10.1007/s00232-008-9120-x"
}

Velebit, J., Kovacic, P. B., Prebil, M., Chowdhury, H. H., Grilc, S., Kreft, M., Jensen, J., Isenović, E. R.,& Zorec, R.. (2008). Rosiglitazone modulates insulin-induced plasma membrane area changes in single 3T3-L1 adipocytes. in Journal of Membrane Biology, 223(3), 141-149.
https://doi.org/10.1007/s00232-008-9120-x

Velebit J, Kovacic PB, Prebil M, Chowdhury HH, Grilc S, Kreft M, Jensen J, Isenović ER, Zorec R. Rosiglitazone modulates insulin-induced plasma membrane area changes in single 3T3-L1 adipocytes. in Journal of Membrane Biology. 2008;223(3):141-149.
doi:10.1007/s00232-008-9120-x .

Velebit, Jelena, Kovacic, Petra Brina, Prebil, Mateja, Chowdhury, Helena H., Grilc, Sonja, Kreft, Marko, Jensen, Jorgen, Isenović, Esma R., Zorec, Robert, "Rosiglitazone modulates insulin-induced plasma membrane area changes in single 3T3-L1 adipocytes" in Journal of Membrane Biology, 223, no. 3 (2008):141-149,
https://doi.org/10.1007/s00232-008-9120-x . .