TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Sudar, Emina
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Milićević, Zorka T.
AU  - Stanimirović, Julijana
AU  - Bogdanović, Nikola
AU  - Isenović, Esma R.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1039
AB  - Alzheimers disease (AD) is a complex and progressive neurodegenerative disorder, and represents the most common form of dementia. The number of people affected by AD is estimated to be doubled by the year of 2050, and more than 100 million people worldwide will be affected by this disease. Still, there is no reliable diagnostic test which would indicate pre-symptomatic conditions or an increased risk of developing AD. The only drugs approved by the FDA belong to the cholinesterase inhibitors (ChEI) group, such as donepezil, rivastigmine, galantamine and memantine that belongs to a class of drugs named receptor NMDA antagonists. Most mainstream pharmacotherapeutic approaches act by slowing the progression of the condition rather than to treat or prevent the cause of AD. In this review we are presenting literature data from recent research related to new avenues in the classical approach to prevention and treatment of AD.
PB  - Bentham Science Publishers
T2  - Current Pharmaceutical Analysis
T1  - Treatment of Alzheimers Disease: Classical Therapeutic Approach
VL  - 12
IS  - 2
SP  - 82
EP  - 90
DO  - 10.2174/1573412911666150611184740
ER  - 

@article{
author = "Bajić, Vladan P. and Sudar, Emina and Spremo-Potparević, Biljana and Živković, Lada and Milićević, Zorka T. and Stanimirović, Julijana and Bogdanović, Nikola and Isenović, Esma R.",
year = "2016",
abstract = "Alzheimers disease (AD) is a complex and progressive neurodegenerative disorder, and represents the most common form of dementia. The number of people affected by AD is estimated to be doubled by the year of 2050, and more than 100 million people worldwide will be affected by this disease. Still, there is no reliable diagnostic test which would indicate pre-symptomatic conditions or an increased risk of developing AD. The only drugs approved by the FDA belong to the cholinesterase inhibitors (ChEI) group, such as donepezil, rivastigmine, galantamine and memantine that belongs to a class of drugs named receptor NMDA antagonists. Most mainstream pharmacotherapeutic approaches act by slowing the progression of the condition rather than to treat or prevent the cause of AD. In this review we are presenting literature data from recent research related to new avenues in the classical approach to prevention and treatment of AD.",
publisher = "Bentham Science Publishers",
journal = "Current Pharmaceutical Analysis",
title = "Treatment of Alzheimers Disease: Classical Therapeutic Approach",
volume = "12",
number = "2",
pages = "82-90",
doi = "10.2174/1573412911666150611184740"
}

Bajić, V. P., Sudar, E., Spremo-Potparević, B., Živković, L., Milićević, Z. T., Stanimirović, J., Bogdanović, N.,& Isenović, E. R.. (2016). Treatment of Alzheimers Disease: Classical Therapeutic Approach. in Current Pharmaceutical Analysis
Bentham Science Publishers., 12(2), 82-90.
https://doi.org/10.2174/1573412911666150611184740

Bajić VP, Sudar E, Spremo-Potparević B, Živković L, Milićević ZT, Stanimirović J, Bogdanović N, Isenović ER. Treatment of Alzheimers Disease: Classical Therapeutic Approach. in Current Pharmaceutical Analysis. 2016;12(2):82-90.
doi:10.2174/1573412911666150611184740 .

Bajić, Vladan P., Sudar, Emina, Spremo-Potparević, Biljana, Živković, Lada, Milićević, Zorka T., Stanimirović, Julijana, Bogdanović, Nikola, Isenović, Esma R., "Treatment of Alzheimers Disease: Classical Therapeutic Approach" in Current Pharmaceutical Analysis, 12, no. 2 (2016):82-90,
https://doi.org/10.2174/1573412911666150611184740 . .

TY  - JOUR
AU  - Milićević, Zorka T.
AU  - Bajić, Vladan P.
AU  - Živković, Lada
AU  - Kasapović, Jelena
AU  - Anđelković, Uros
AU  - Spremo-Potparević, Biljana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5855
AB  - In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms Lambda Np53 (47 kDa) and Lambda 133p53 beta (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer.
T2  - Scientific World Journal
T1  - Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells
DO  - 10.1155/2014/618698
ER  - 

@article{
author = "Milićević, Zorka T. and Bajić, Vladan P. and Živković, Lada and Kasapović, Jelena and Anđelković, Uros and Spremo-Potparević, Biljana",
year = "2014",
abstract = "In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms Lambda Np53 (47 kDa) and Lambda 133p53 beta (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer.",
journal = "Scientific World Journal",
title = "Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells",
doi = "10.1155/2014/618698"
}

Milićević, Z. T., Bajić, V. P., Živković, L., Kasapović, J., Anđelković, U.,& Spremo-Potparević, B.. (2014). Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells. in Scientific World Journal.
https://doi.org/10.1155/2014/618698

Milićević ZT, Bajić VP, Živković L, Kasapović J, Anđelković U, Spremo-Potparević B. Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells. in Scientific World Journal. 2014;.
doi:10.1155/2014/618698 .

Milićević, Zorka T., Bajić, Vladan P., Živković, Lada, Kasapović, Jelena, Anđelković, Uros, Spremo-Potparević, Biljana, "Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells" in Scientific World Journal (2014),
https://doi.org/10.1155/2014/618698 . .

TY  - JOUR
AU  - Milićević, Zorka T.
AU  - Kasapović, Jelena
AU  - Gavrilović, Ljubica
AU  - Milovanović, Zorka M.
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/113
AB  - It is well recognized that cancers develop and grow as a result of disordered function of tumor suppressor genes and oncogenes, which may be exploited for screening purposes. Extensive evidence indicated tumor suppressor protein p53 as candidate marker for mutation identification. We have investigated mutant p53 protein expression in human breast tumors in relation to antioxidant status deficiency. The study included 100 breast cancer patients. p53 protein expression was evaluated by Western blot assay and immunostaining using a CM-1, DO-7 and Pab240 antibodies. Antioxidant parameters and lipid peroxidation were estimated by biochemical analyses. Western blotting with epitope-specific monoclonal antibody Pab240 strongly suggests that nuclear extracts from breast cancer cells express mutant forms of p53. It is of interest that the mutant forms of p53 overexpression in conjunction with the appearance of nuclear bodies are observed in highly aggressive carcinomas. Expression of isoform Delta p53 (45 kDa) and isoform of similar to 29 kDa were more common in cases with LN metastasis. These studies point out the molecular consequences of oxidative stress (lipid peroxides, LP, p LT 0.001) and antioxidant status deficiency (copper, zinc superoxid dismutase, SOD, p LT 0.001; catalase, CAT, p LT 0.01; glutathione reductase, GR, p LT 0.001; glutathione, GSH, p LT 0.05) and indicate the importance of p53 mutation as the commonest genetic alteration detected in breast cancer cells. The expression of mutant p53 is correlated to increased lipid peroxides (0.346, p LT 0.05) and lowered antioxidant activity of CAT (- 0.437, p LT 0.01) in the breast cancer patients.
T2  - EXCLI Journal
T1  - Mutant P53 Protein Expression and Antioxidant Status Deficiency in Breast Cancer
VL  - 13
SP  - 691
EP  - 708
UR  - https://hdl.handle.net/21.15107/rcub_vinar_113
ER  - 

@article{
author = "Milićević, Zorka T. and Kasapović, Jelena and Gavrilović, Ljubica and Milovanović, Zorka M. and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2014",
abstract = "It is well recognized that cancers develop and grow as a result of disordered function of tumor suppressor genes and oncogenes, which may be exploited for screening purposes. Extensive evidence indicated tumor suppressor protein p53 as candidate marker for mutation identification. We have investigated mutant p53 protein expression in human breast tumors in relation to antioxidant status deficiency. The study included 100 breast cancer patients. p53 protein expression was evaluated by Western blot assay and immunostaining using a CM-1, DO-7 and Pab240 antibodies. Antioxidant parameters and lipid peroxidation were estimated by biochemical analyses. Western blotting with epitope-specific monoclonal antibody Pab240 strongly suggests that nuclear extracts from breast cancer cells express mutant forms of p53. It is of interest that the mutant forms of p53 overexpression in conjunction with the appearance of nuclear bodies are observed in highly aggressive carcinomas. Expression of isoform Delta p53 (45 kDa) and isoform of similar to 29 kDa were more common in cases with LN metastasis. These studies point out the molecular consequences of oxidative stress (lipid peroxides, LP, p LT 0.001) and antioxidant status deficiency (copper, zinc superoxid dismutase, SOD, p LT 0.001; catalase, CAT, p LT 0.01; glutathione reductase, GR, p LT 0.001; glutathione, GSH, p LT 0.05) and indicate the importance of p53 mutation as the commonest genetic alteration detected in breast cancer cells. The expression of mutant p53 is correlated to increased lipid peroxides (0.346, p LT 0.05) and lowered antioxidant activity of CAT (- 0.437, p LT 0.01) in the breast cancer patients.",
journal = "EXCLI Journal",
title = "Mutant P53 Protein Expression and Antioxidant Status Deficiency in Breast Cancer",
volume = "13",
pages = "691-708",
url = "https://hdl.handle.net/21.15107/rcub_vinar_113"
}

Milićević, Z. T., Kasapović, J., Gavrilović, L., Milovanović, Z. M., Bajić, V. P.,& Spremo-Potparević, B.. (2014). Mutant P53 Protein Expression and Antioxidant Status Deficiency in Breast Cancer. in EXCLI Journal, 13, 691-708.
https://hdl.handle.net/21.15107/rcub_vinar_113

Milićević ZT, Kasapović J, Gavrilović L, Milovanović ZM, Bajić VP, Spremo-Potparević B. Mutant P53 Protein Expression and Antioxidant Status Deficiency in Breast Cancer. in EXCLI Journal. 2014;13:691-708.
https://hdl.handle.net/21.15107/rcub_vinar_113 .

Milićević, Zorka T., Kasapović, Jelena, Gavrilović, Ljubica, Milovanović, Zorka M., Bajić, Vladan P., Spremo-Potparević, Biljana, "Mutant P53 Protein Expression and Antioxidant Status Deficiency in Breast Cancer" in EXCLI Journal, 13 (2014):691-708,
https://hdl.handle.net/21.15107/rcub_vinar_113 .

TY  - JOUR
AU  - Kasapović, Jelena
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Milićević, Zorka T.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4959
AB  - Reactive oxygen species (ROS) are independently recognized to play a significant role in radiation-induced damage on healthy tissue and in aging process. However, an age-related alteration of antioxidant (AO) system in radiation response in humans is poorly investigated. The aim of this paper was to evaluate the irradiation effects on the activities and expression of AO system in the blood of healthy women during aging. Blood samples were irradiated with curative and palliative doses of 2Gy or 9Gy gamma-rays. AO capacity for detoxification of O-2 center dot(-) and H2O2 in response to 2Gy gamma-irradiation decreases in women above 58 years, while in response to 9Gy shows signs of weakening after 45 years of age. Due to reduction of AO capacity during aging, cytotoxic effects of curative and palliative doses of irradiation, mediated by ROS, may significantly increase in older subjects, while removal of H2O2 excess could reduce them.
T2  - Scientific World Journal
T1  - Antioxidant Protection against Curative and Palliative Doses of Ionizing Irradiation in Human Blood Decreases with Aging
DO  - 10.1100/2012/982594
ER  - 

@article{
author = "Kasapović, Jelena and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Milićević, Zorka T.",
year = "2012",
abstract = "Reactive oxygen species (ROS) are independently recognized to play a significant role in radiation-induced damage on healthy tissue and in aging process. However, an age-related alteration of antioxidant (AO) system in radiation response in humans is poorly investigated. The aim of this paper was to evaluate the irradiation effects on the activities and expression of AO system in the blood of healthy women during aging. Blood samples were irradiated with curative and palliative doses of 2Gy or 9Gy gamma-rays. AO capacity for detoxification of O-2 center dot(-) and H2O2 in response to 2Gy gamma-irradiation decreases in women above 58 years, while in response to 9Gy shows signs of weakening after 45 years of age. Due to reduction of AO capacity during aging, cytotoxic effects of curative and palliative doses of irradiation, mediated by ROS, may significantly increase in older subjects, while removal of H2O2 excess could reduce them.",
journal = "Scientific World Journal",
title = "Antioxidant Protection against Curative and Palliative Doses of Ionizing Irradiation in Human Blood Decreases with Aging",
doi = "10.1100/2012/982594"
}

Kasapović, J., Stojiljković, V., Gavrilović, L., Popović, N. M.,& Milićević, Z. T.. (2012). Antioxidant Protection against Curative and Palliative Doses of Ionizing Irradiation in Human Blood Decreases with Aging. in Scientific World Journal.
https://doi.org/10.1100/2012/982594

Kasapović J, Stojiljković V, Gavrilović L, Popović NM, Milićević ZT. Antioxidant Protection against Curative and Palliative Doses of Ionizing Irradiation in Human Blood Decreases with Aging. in Scientific World Journal. 2012;.
doi:10.1100/2012/982594 .

Kasapović, Jelena, Stojiljković, Vesna, Gavrilović, Ljubica, Popović, Nataša M., Milićević, Zorka T., "Antioxidant Protection against Curative and Palliative Doses of Ionizing Irradiation in Human Blood Decreases with Aging" in Scientific World Journal (2012),
https://doi.org/10.1100/2012/982594 . .

TY  - CONF
AU  - Milićević, Zorka T.
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6928
C3  - European Journal of Neurology
T1  - Chaperone Proteins Associate with B-Amyloid Peptide (Ab) as Part of Multicomponent Complex
VL  - 18
SP  - 357
EP  - 357
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6928
ER  - 

@conference{
author = "Milićević, Zorka T. and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2011",
journal = "European Journal of Neurology",
title = "Chaperone Proteins Associate with B-Amyloid Peptide (Ab) as Part of Multicomponent Complex",
volume = "18",
pages = "357-357",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6928"
}

Milićević, Z. T., Bajić, V. P.,& Spremo-Potparević, B.. (2011). Chaperone Proteins Associate with B-Amyloid Peptide (Ab) as Part of Multicomponent Complex. in European Journal of Neurology, 18, 357-357.
https://hdl.handle.net/21.15107/rcub_vinar_6928

Milićević ZT, Bajić VP, Spremo-Potparević B. Chaperone Proteins Associate with B-Amyloid Peptide (Ab) as Part of Multicomponent Complex. in European Journal of Neurology. 2011;18:357-357.
https://hdl.handle.net/21.15107/rcub_vinar_6928 .

Milićević, Zorka T., Bajić, Vladan P., Spremo-Potparević, Biljana, "Chaperone Proteins Associate with B-Amyloid Peptide (Ab) as Part of Multicomponent Complex" in European Journal of Neurology, 18 (2011):357-357,
https://hdl.handle.net/21.15107/rcub_vinar_6928 .

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Stanimirovic, Z.
AU  - Stevanović, Jevrosima
AU  - Milićević, Zorka T.
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3998
AB  - Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono- and/or combinational therapies for cancer treatment.
T2  - Archives of Biological Sciences
T1  - The Effect of Paclitaxel Alone and in Combination with Cycloheximide on the Frequency of Premature Centromere Division in Vitro
VL  - 62
IS  - 1
SP  - 63
EP  - 74
DO  - 10.2298/ABS1001063B
ER  - 

@article{
author = "Bajić, Vladan P. and Stanimirovic, Z. and Stevanović, Jevrosima and Milićević, Zorka T. and Živković, Lada and Spremo-Potparević, Biljana",
year = "2010",
abstract = "Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono- and/or combinational therapies for cancer treatment.",
journal = "Archives of Biological Sciences",
title = "The Effect of Paclitaxel Alone and in Combination with Cycloheximide on the Frequency of Premature Centromere Division in Vitro",
volume = "62",
number = "1",
pages = "63-74",
doi = "10.2298/ABS1001063B"
}

Bajić, V. P., Stanimirovic, Z., Stevanović, J., Milićević, Z. T., Živković, L.,& Spremo-Potparević, B.. (2010). The Effect of Paclitaxel Alone and in Combination with Cycloheximide on the Frequency of Premature Centromere Division in Vitro. in Archives of Biological Sciences, 62(1), 63-74.
https://doi.org/10.2298/ABS1001063B

Bajić VP, Stanimirovic Z, Stevanović J, Milićević ZT, Živković L, Spremo-Potparević B. The Effect of Paclitaxel Alone and in Combination with Cycloheximide on the Frequency of Premature Centromere Division in Vitro. in Archives of Biological Sciences. 2010;62(1):63-74.
doi:10.2298/ABS1001063B .

Bajić, Vladan P., Stanimirovic, Z., Stevanović, Jevrosima, Milićević, Zorka T., Živković, Lada, Spremo-Potparević, Biljana, "The Effect of Paclitaxel Alone and in Combination with Cycloheximide on the Frequency of Premature Centromere Division in Vitro" in Archives of Biological Sciences, 62, no. 1 (2010):63-74,
https://doi.org/10.2298/ABS1001063B . .

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Stanimirovic, Z.
AU  - Stevanović, Jasmina
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Milićević, Zorka T.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3670
AB  - Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3, 5 cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assail (CA) on human peripheral blood lymphocytes of healthy individuals and by hone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 pin) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p) injection of 8-Cl-cAMP in doses of 10, 80 and 15 0 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.
T2  - Journal of BUON
T1  - Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes
VL  - 14
IS  - 1
SP  - 71
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3670
ER  - 

@article{
author = "Bajić, Vladan P. and Stanimirovic, Z. and Stevanović, Jasmina and Spremo-Potparević, Biljana and Živković, Lada and Milićević, Zorka T.",
year = "2009",
abstract = "Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3, 5 cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assail (CA) on human peripheral blood lymphocytes of healthy individuals and by hone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 pin) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p) injection of 8-Cl-cAMP in doses of 10, 80 and 15 0 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.",
journal = "Journal of BUON",
title = "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes",
volume = "14",
number = "1",
pages = "71-77",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3670"
}

Bajić, V. P., Stanimirovic, Z., Stevanović, J., Spremo-Potparević, B., Živković, L.,& Milićević, Z. T.. (2009). Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON, 14(1), 71-77.
https://hdl.handle.net/21.15107/rcub_vinar_3670

Bajić VP, Stanimirovic Z, Stevanović J, Spremo-Potparević B, Živković L, Milićević ZT. Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON. 2009;14(1):71-77.
https://hdl.handle.net/21.15107/rcub_vinar_3670 .

Bajić, Vladan P., Stanimirovic, Z., Stevanović, Jasmina, Spremo-Potparević, Biljana, Živković, Lada, Milićević, Zorka T., "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes" in Journal of BUON, 14, no. 1 (2009):71-77,
https://hdl.handle.net/21.15107/rcub_vinar_3670 .

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Djelic, N.
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Milićević, Zorka T.
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3450
AB  - 8-chloro-cyclic adenosine 3,5-monophosphate (8-Cl-cAMP) is the most potent cAMP analog that selectively inhibits a variety of cancer cell lines in vitro and tumors in vivo. Its action toward a variety of tumors, especially when coupled with other antitumor agents, have lead to phase I clinical investigations and recently phase II clinical investigations. Until today, very little was done to evaluate its genotoxic potential. In order to evaluate its genotoxic potential we used the cytogenetic and cytokinesis block micronucleus assay in vitro on peripheral blood lymphocytes of healthy individuals. In three concentrations (1 mu M, 5 mu M and 15 mu M), 8-Cl-cAMP in normal human peripheral blood lymphocytes did not induce any cytogenetic aberrations of the structural type (chromatid breakage, isochromatid breakage and gaps), but did induce premature centromere separation (PCS) at all respective doses and increased the frequency of micronuclei (p LT 0.05) only at the highest dose (15 mu M). Antiproliferative action of 8-Cl-cAMP was estimated by using the cytokinesis block nuclear division index (NDI). The results showed a decrease in NDI of cells exposed to all doses of 8-Cl-cAMP when compared to control. Therefore, the overall results show a genotoxic potential of 8-Cl-cAMP in peripheral blood lymphocytes in vitro.
T2  - Russian Journal of Genetics
T1  - A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro
VL  - 44
IS  - 5
SP  - 546
EP  - 552
DO  - 10.1134/S1022795408050062
ER  - 

@article{
author = "Bajić, Vladan P. and Djelic, N. and Spremo-Potparević, Biljana and Živković, Lada and Milićević, Zorka T.",
year = "2008",
abstract = "8-chloro-cyclic adenosine 3,5-monophosphate (8-Cl-cAMP) is the most potent cAMP analog that selectively inhibits a variety of cancer cell lines in vitro and tumors in vivo. Its action toward a variety of tumors, especially when coupled with other antitumor agents, have lead to phase I clinical investigations and recently phase II clinical investigations. Until today, very little was done to evaluate its genotoxic potential. In order to evaluate its genotoxic potential we used the cytogenetic and cytokinesis block micronucleus assay in vitro on peripheral blood lymphocytes of healthy individuals. In three concentrations (1 mu M, 5 mu M and 15 mu M), 8-Cl-cAMP in normal human peripheral blood lymphocytes did not induce any cytogenetic aberrations of the structural type (chromatid breakage, isochromatid breakage and gaps), but did induce premature centromere separation (PCS) at all respective doses and increased the frequency of micronuclei (p LT 0.05) only at the highest dose (15 mu M). Antiproliferative action of 8-Cl-cAMP was estimated by using the cytokinesis block nuclear division index (NDI). The results showed a decrease in NDI of cells exposed to all doses of 8-Cl-cAMP when compared to control. Therefore, the overall results show a genotoxic potential of 8-Cl-cAMP in peripheral blood lymphocytes in vitro.",
journal = "Russian Journal of Genetics",
title = "A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro",
volume = "44",
number = "5",
pages = "546-552",
doi = "10.1134/S1022795408050062"
}

Bajić, V. P., Djelic, N., Spremo-Potparević, B., Živković, L.,& Milićević, Z. T.. (2008). A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro. in Russian Journal of Genetics, 44(5), 546-552.
https://doi.org/10.1134/S1022795408050062

Bajić VP, Djelic N, Spremo-Potparević B, Živković L, Milićević ZT. A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro. in Russian Journal of Genetics. 2008;44(5):546-552.
doi:10.1134/S1022795408050062 .

Bajić, Vladan P., Djelic, N., Spremo-Potparević, Biljana, Živković, Lada, Milićević, Zorka T., "A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro" in Russian Journal of Genetics, 44, no. 5 (2008):546-552,
https://doi.org/10.1134/S1022795408050062 . .

TY  - JOUR
AU  - Milićević, Zorka T.
AU  - Bogojevic, D.
AU  - Mihailovic, M.
AU  - Petrović, Milica S.
AU  - Krivokapic, Z.
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3456
AB  - A key role of hsp90 in the activity of various oncogenic proteins and pathways is currently of intense interest. To clarify the molecular basis of biological behaviour of colorectal cancers we analysed the expression characteristics of hsp90 in cytosolic, nuclear and plasma membranous fractions of cancer cells. As determined by Western blot assay all hsp90 isoforms studied, a (84 kDa), 8 (86 kDa) and hsp90N (75 kDa), were up-regulated and differentially expressed in various stages of colorectal carcinoma. The inducible hsp90 alpha isoform is a component of invasive phenotype of cancer cells thus pointing to the importance of hsp90 alpha for metastasis generation. The expression of hsp90 beta is definitely higher in poorly-differentiated carcinomas than in well-differentiated cancers, suggesting an involvement of hsp90 beta in the inhibition of cancer cell differentiation. Especially, the expression of cytosolic hsp90N isoform in malignant cells points to the possibility that induction or overexpression of hsp90N might be causally related to tumour formation. Hsp90N is the plasma-membrane-associated protein in poorly-differentiated colorectal cancers with metastasis. This suggests that the expression of hsp90N is elevated with progressive dedifferentiation often associated with advanced cancer stages. Hsp90 was exclusively localized in the invasive front in a majority of metastatic cancers as visualized by immunohistochemical study. Consistent with these facts, the frequent expression of hsp90 alpha and hsp90N on the surface of colorectal cancer cells may enable hsp90 to act as a mediator of metastasis generation. The above results indicate more complex roles for hsp90 in colorectal tumourigenesis. In this way, the hsp90 would be at the crossroads of both signalling and cell migration events.
T2  - International Journal of Oncology
T1  - Molecular characterization of hsp90 isoforms in colorectal cancer cells and its association with tumour progression
VL  - 32
IS  - 6
SP  - 1169
EP  - 1178
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3456
ER  - 

@article{
author = "Milićević, Zorka T. and Bogojevic, D. and Mihailovic, M. and Petrović, Milica S. and Krivokapic, Z.",
year = "2008",
abstract = "A key role of hsp90 in the activity of various oncogenic proteins and pathways is currently of intense interest. To clarify the molecular basis of biological behaviour of colorectal cancers we analysed the expression characteristics of hsp90 in cytosolic, nuclear and plasma membranous fractions of cancer cells. As determined by Western blot assay all hsp90 isoforms studied, a (84 kDa), 8 (86 kDa) and hsp90N (75 kDa), were up-regulated and differentially expressed in various stages of colorectal carcinoma. The inducible hsp90 alpha isoform is a component of invasive phenotype of cancer cells thus pointing to the importance of hsp90 alpha for metastasis generation. The expression of hsp90 beta is definitely higher in poorly-differentiated carcinomas than in well-differentiated cancers, suggesting an involvement of hsp90 beta in the inhibition of cancer cell differentiation. Especially, the expression of cytosolic hsp90N isoform in malignant cells points to the possibility that induction or overexpression of hsp90N might be causally related to tumour formation. Hsp90N is the plasma-membrane-associated protein in poorly-differentiated colorectal cancers with metastasis. This suggests that the expression of hsp90N is elevated with progressive dedifferentiation often associated with advanced cancer stages. Hsp90 was exclusively localized in the invasive front in a majority of metastatic cancers as visualized by immunohistochemical study. Consistent with these facts, the frequent expression of hsp90 alpha and hsp90N on the surface of colorectal cancer cells may enable hsp90 to act as a mediator of metastasis generation. The above results indicate more complex roles for hsp90 in colorectal tumourigenesis. In this way, the hsp90 would be at the crossroads of both signalling and cell migration events.",
journal = "International Journal of Oncology",
title = "Molecular characterization of hsp90 isoforms in colorectal cancer cells and its association with tumour progression",
volume = "32",
number = "6",
pages = "1169-1178",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3456"
}

Milićević, Z. T., Bogojevic, D., Mihailovic, M., Petrović, M. S.,& Krivokapic, Z.. (2008). Molecular characterization of hsp90 isoforms in colorectal cancer cells and its association with tumour progression. in International Journal of Oncology, 32(6), 1169-1178.
https://hdl.handle.net/21.15107/rcub_vinar_3456

Milićević ZT, Bogojevic D, Mihailovic M, Petrović MS, Krivokapic Z. Molecular characterization of hsp90 isoforms in colorectal cancer cells and its association with tumour progression. in International Journal of Oncology. 2008;32(6):1169-1178.
https://hdl.handle.net/21.15107/rcub_vinar_3456 .

Milićević, Zorka T., Bogojevic, D., Mihailovic, M., Petrović, Milica S., Krivokapic, Z., "Molecular characterization of hsp90 isoforms in colorectal cancer cells and its association with tumour progression" in International Journal of Oncology, 32, no. 6 (2008):1169-1178,
https://hdl.handle.net/21.15107/rcub_vinar_3456 .

TY  - JOUR
AU  - Milićević, Zorka T.
AU  - Petković, Marijana
AU  - Drndarević, Neda C.
AU  - Pavlović, Mirjana D.
AU  - Todorović, Vera N.
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3188
AB  - The role of heat shock protein 70 (HSP70) expression has been investigated in various types of tumors. There are only little and controversial data about its clinical relevance in colorectal carcinoma, one of the most common carcinomas observed in humans. In this study we investigated expression of HSP70 in human colonic carcinoma and possible correlation with clinicopathology. To assess patterns (cytosolic and membrane) of HSP70 expression, the 48 surgically removed colorectal adenocarcinomas and 12 normal colonic and rectal mucosal samples were examined by immunohistochemistry and Western-blot. According to results of immunohistochemistry, expression of cytoplasmic HSP72 was significantly higher in colorectal carcinoma compared with normal and adjacent mucosa (p LT 0.01). In addition, there was significant increase in HSP72 expression in lymph node-positive compared to node-nevative group (p LT 0.001). Dukes C2 stage of colonic cancer showed significantly higher immunohistochemical score than Dukes B2 and B1 stage groups (p LT 0.05 i.e. p LT 0.02). There was no relation between expression of HSP72 and degree of tumor differentiation. Using Western blot analyses, we noticed elevated levels of cytosolic HSP70 in colorectal cancer cells compared to normal. Densitometric analysis of blots of plasma membrane HSP70 expression has shown decrease in colorectal cancer cells compared to normal mucosa. According to our results, overexpression of HSP72 in malignant tissues of patients with colorectal carcinoma is related to tumor progression, suggesting that these proteins could play an important role not only in tumorigenesis but also in the development of drug resistance. Further research is necessary to clarify the mechanisms responsible for differential HSP70 expression as well as its definitive role in colorectal cancer.
T2  - Neoplasma
T1  - Expression of heat shock protein 70 (HSP70) in patients with colorectal adenocarcinoma - immunohistochemistry and Western blot analysis
VL  - 54
IS  - 1
SP  - 37
EP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3188
ER  - 

@article{
author = "Milićević, Zorka T. and Petković, Marijana and Drndarević, Neda C. and Pavlović, Mirjana D. and Todorović, Vera N.",
year = "2007",
abstract = "The role of heat shock protein 70 (HSP70) expression has been investigated in various types of tumors. There are only little and controversial data about its clinical relevance in colorectal carcinoma, one of the most common carcinomas observed in humans. In this study we investigated expression of HSP70 in human colonic carcinoma and possible correlation with clinicopathology. To assess patterns (cytosolic and membrane) of HSP70 expression, the 48 surgically removed colorectal adenocarcinomas and 12 normal colonic and rectal mucosal samples were examined by immunohistochemistry and Western-blot. According to results of immunohistochemistry, expression of cytoplasmic HSP72 was significantly higher in colorectal carcinoma compared with normal and adjacent mucosa (p LT 0.01). In addition, there was significant increase in HSP72 expression in lymph node-positive compared to node-nevative group (p LT 0.001). Dukes C2 stage of colonic cancer showed significantly higher immunohistochemical score than Dukes B2 and B1 stage groups (p LT 0.05 i.e. p LT 0.02). There was no relation between expression of HSP72 and degree of tumor differentiation. Using Western blot analyses, we noticed elevated levels of cytosolic HSP70 in colorectal cancer cells compared to normal. Densitometric analysis of blots of plasma membrane HSP70 expression has shown decrease in colorectal cancer cells compared to normal mucosa. According to our results, overexpression of HSP72 in malignant tissues of patients with colorectal carcinoma is related to tumor progression, suggesting that these proteins could play an important role not only in tumorigenesis but also in the development of drug resistance. Further research is necessary to clarify the mechanisms responsible for differential HSP70 expression as well as its definitive role in colorectal cancer.",
journal = "Neoplasma",
title = "Expression of heat shock protein 70 (HSP70) in patients with colorectal adenocarcinoma - immunohistochemistry and Western blot analysis",
volume = "54",
number = "1",
pages = "37-45",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3188"
}

Milićević, Z. T., Petković, M., Drndarević, N. C., Pavlović, M. D.,& Todorović, V. N.. (2007). Expression of heat shock protein 70 (HSP70) in patients with colorectal adenocarcinoma - immunohistochemistry and Western blot analysis. in Neoplasma, 54(1), 37-45.
https://hdl.handle.net/21.15107/rcub_vinar_3188

Milićević ZT, Petković M, Drndarević NC, Pavlović MD, Todorović VN. Expression of heat shock protein 70 (HSP70) in patients with colorectal adenocarcinoma - immunohistochemistry and Western blot analysis. in Neoplasma. 2007;54(1):37-45.
https://hdl.handle.net/21.15107/rcub_vinar_3188 .

Milićević, Zorka T., Petković, Marijana, Drndarević, Neda C., Pavlović, Mirjana D., Todorović, Vera N., "Expression of heat shock protein 70 (HSP70) in patients with colorectal adenocarcinoma - immunohistochemistry and Western blot analysis" in Neoplasma, 54, no. 1 (2007):37-45,
https://hdl.handle.net/21.15107/rcub_vinar_3188 .

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
AU  - Milićević, Zorka T.
AU  - Živković, Lada
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3462
AB  - Purpose: Segregation of chromosomes in anaphase is preceded by a sequential order of centromere separation. Alteration of the sequence of centromere separation or premature centromere division (PCD) has been found to be significantly higher in populations exposed to various xenobiotics. The purpose of this study was to investigate if PCD induced by various cytostatics can alter the stability of chromosomes and lead to aneuploidy. Materials and methods: Peripheral blood lymphocytes of 10 healthy, non smoking subjects were exposed to 8-Cl-cAMP at a dose of 1, 5 and 15 mu M, paclitaxel at a dose of 0.01, 0.05 and 0.2 mu M, and cycloheximide (CX) at a dose of 5, 10 and 25 mu g/ml. By using the cytohalasin B (CB)-micronucleus (MN) test in vitro, in combination with fluorescent in situ hybridization (FISH), the presence of MN was analyzed in 1000 binuclear cells for each experimental and negative control group. For analysis of MN content we used the a-centromeric probe for chromosome 18. Results: 8-Cl-cAMP and paclitaxel induced an increase in the frequency of MN in peripheral blood lymphocytes. 8-Cl-cAMP and paclitaxel proved clastogenic, i.e. they increased the frequency of MN and induced PCD in all respective doses. CX proved no clastogenic in the respected doses when using the CB-AM test in vitro, although CX is a specific PCD inducer No correlation of PCD and aneuploidy of chromosome 18 was found in cells exposed to 8-Cl-cAmp and paclitaxel by using FISH. In cells exposed to CX we found PCD of chromosome 18 in binuclear cells and single signals in scarce AN. These findings were not statistically significant compared to the negative control group. Conclusion: Our results show that the properties of the investigated antitumor agents to induce PCD in peripheral blood lymphocytes and, therefore, aneuploidy and genome instability, is highly based on the nature of the alteration of centromere function, i.e. the temporal order of centromere kinetics are more regulated through the sequence of centromere separation than by the segregation processes. We suggest that PCD induced by novel antitumor agents could be included in preclinical and clinical genetic risk assessment analysis.
T2  - Journal of BUON
T1  - Deregulated sequential motion of centromeres induced by antitumor agents may lead to genome instability in human peripheral blood lymphocytes
VL  - 12
IS  - 1
SP  - 77
EP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3462
ER  - 

@article{
author = "Bajić, Vladan P. and Spremo-Potparević, Biljana and Milićević, Zorka T. and Živković, Lada",
year = "2007",
abstract = "Purpose: Segregation of chromosomes in anaphase is preceded by a sequential order of centromere separation. Alteration of the sequence of centromere separation or premature centromere division (PCD) has been found to be significantly higher in populations exposed to various xenobiotics. The purpose of this study was to investigate if PCD induced by various cytostatics can alter the stability of chromosomes and lead to aneuploidy. Materials and methods: Peripheral blood lymphocytes of 10 healthy, non smoking subjects were exposed to 8-Cl-cAMP at a dose of 1, 5 and 15 mu M, paclitaxel at a dose of 0.01, 0.05 and 0.2 mu M, and cycloheximide (CX) at a dose of 5, 10 and 25 mu g/ml. By using the cytohalasin B (CB)-micronucleus (MN) test in vitro, in combination with fluorescent in situ hybridization (FISH), the presence of MN was analyzed in 1000 binuclear cells for each experimental and negative control group. For analysis of MN content we used the a-centromeric probe for chromosome 18. Results: 8-Cl-cAMP and paclitaxel induced an increase in the frequency of MN in peripheral blood lymphocytes. 8-Cl-cAMP and paclitaxel proved clastogenic, i.e. they increased the frequency of MN and induced PCD in all respective doses. CX proved no clastogenic in the respected doses when using the CB-AM test in vitro, although CX is a specific PCD inducer No correlation of PCD and aneuploidy of chromosome 18 was found in cells exposed to 8-Cl-cAmp and paclitaxel by using FISH. In cells exposed to CX we found PCD of chromosome 18 in binuclear cells and single signals in scarce AN. These findings were not statistically significant compared to the negative control group. Conclusion: Our results show that the properties of the investigated antitumor agents to induce PCD in peripheral blood lymphocytes and, therefore, aneuploidy and genome instability, is highly based on the nature of the alteration of centromere function, i.e. the temporal order of centromere kinetics are more regulated through the sequence of centromere separation than by the segregation processes. We suggest that PCD induced by novel antitumor agents could be included in preclinical and clinical genetic risk assessment analysis.",
journal = "Journal of BUON",
title = "Deregulated sequential motion of centromeres induced by antitumor agents may lead to genome instability in human peripheral blood lymphocytes",
volume = "12",
number = "1",
pages = "77-83",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3462"
}

Bajić, V. P., Spremo-Potparević, B., Milićević, Z. T.,& Živković, L.. (2007). Deregulated sequential motion of centromeres induced by antitumor agents may lead to genome instability in human peripheral blood lymphocytes. in Journal of BUON, 12(1), 77-83.
https://hdl.handle.net/21.15107/rcub_vinar_3462

Bajić VP, Spremo-Potparević B, Milićević ZT, Živković L. Deregulated sequential motion of centromeres induced by antitumor agents may lead to genome instability in human peripheral blood lymphocytes. in Journal of BUON. 2007;12(1):77-83.
https://hdl.handle.net/21.15107/rcub_vinar_3462 .

Bajić, Vladan P., Spremo-Potparević, Biljana, Milićević, Zorka T., Živković, Lada, "Deregulated sequential motion of centromeres induced by antitumor agents may lead to genome instability in human peripheral blood lymphocytes" in Journal of BUON, 12, no. 1 (2007):77-83,
https://hdl.handle.net/21.15107/rcub_vinar_3462 .

TY  - CONF
AU  - Milićević, Zorka T.
AU  - Petrovic, MV
AU  - Mihailovic, MV
AU  - Bogojevic, DB
PY  - 2005
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6562
C3  - FEBS Journal
T1  - Cytoplasmic retention of p53 in colorectal cancer cells
VL  - 272
SP  - 127
EP  - 128
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6562
ER  - 

@conference{
author = "Milićević, Zorka T. and Petrovic, MV and Mihailovic, MV and Bogojevic, DB",
year = "2005",
journal = "FEBS Journal",
title = "Cytoplasmic retention of p53 in colorectal cancer cells",
volume = "272",
pages = "127-128",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6562"
}

Milićević, Z. T., Petrovic, M., Mihailovic, M.,& Bogojevic, D.. (2005). Cytoplasmic retention of p53 in colorectal cancer cells. in FEBS Journal, 272, 127-128.
https://hdl.handle.net/21.15107/rcub_vinar_6562

Milićević ZT, Petrovic M, Mihailovic M, Bogojevic D. Cytoplasmic retention of p53 in colorectal cancer cells. in FEBS Journal. 2005;272:127-128.
https://hdl.handle.net/21.15107/rcub_vinar_6562 .

Milićević, Zorka T., Petrovic, MV, Mihailovic, MV, Bogojevic, DB, "Cytoplasmic retention of p53 in colorectal cancer cells" in FEBS Journal, 272 (2005):127-128,
https://hdl.handle.net/21.15107/rcub_vinar_6562 .

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Milićević, Zorka T.
AU  - Spremo-Potparević, Biljana
AU  - Nedeljkovic-Kurepa, A
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2683
T2  - Medical Hypotheses
T1  - Corrigendum: Apoptotic versus genotoxic potential of antiotumor agents: a concept of duality in unity (vol 61, pg 131, 2003)
VL  - 61
IS  - 5-6
SP  - 662
EP  - 662
DO  - 10.1016/S0306-9877(03)00289-5
ER  - 

@article{
author = "Bajić, Vladan P. and Milićević, Zorka T. and Spremo-Potparević, Biljana and Nedeljkovic-Kurepa, A",
year = "2003",
journal = "Medical Hypotheses",
title = "Corrigendum: Apoptotic versus genotoxic potential of antiotumor agents: a concept of duality in unity (vol 61, pg 131, 2003)",
volume = "61",
number = "5-6",
pages = "662-662",
doi = "10.1016/S0306-9877(03)00289-5"
}

Bajić, V. P., Milićević, Z. T., Spremo-Potparević, B.,& Nedeljkovic-Kurepa, A.. (2003). Corrigendum: Apoptotic versus genotoxic potential of antiotumor agents: a concept of duality in unity (vol 61, pg 131, 2003). in Medical Hypotheses, 61(5-6), 662-662.
https://doi.org/10.1016/S0306-9877(03)00289-5

Bajić VP, Milićević ZT, Spremo-Potparević B, Nedeljkovic-Kurepa A. Corrigendum: Apoptotic versus genotoxic potential of antiotumor agents: a concept of duality in unity (vol 61, pg 131, 2003). in Medical Hypotheses. 2003;61(5-6):662-662.
doi:10.1016/S0306-9877(03)00289-5 .

Bajić, Vladan P., Milićević, Zorka T., Spremo-Potparević, Biljana, Nedeljkovic-Kurepa, A, "Corrigendum: Apoptotic versus genotoxic potential of antiotumor agents: a concept of duality in unity (vol 61, pg 131, 2003)" in Medical Hypotheses, 61, no. 5-6 (2003):662-662,
https://doi.org/10.1016/S0306-9877(03)00289-5 . .

TY  - JOUR
AU  - Vladan, B
AU  - Milićević, Zorka T.
AU  - Biljana, PS
AU  - Nedeljkovic-Kurepa, A
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2682
AB  - Recent advances in anti-tumor therapy have raised a problem of secondary tumors and tumor resistance. Secondary tumors induced by chemotherapeutic agents as a consequence of primary therapy have poor prognostic outcome. Many new insights into molecular controls of cell cycle progression of normal and cancer cells can provide a useful framework in order to identify potential targets for anti-tumor therapies. One of the most promising strategies is the possibility to modulate apoptosis induced by anti-tumor agents. Cancer cell survival after chemotherapy will depend on specific checkpoints and/or repair pathways that have been lost, leading either to greater susceptibility to anti-tumor agents when the repair of damage is most important for survival or to greater resistance when the apoptotic response is more important. We have proposed a hypothesis that views survival and apoptotic processes (duality) in normal and tumor cells as genetically coupled (unity). We introduce, through a theoretical background, a new pathway of apoptotic inhibition. The proposed process of apoptotic inhibition is induced by mutation fixation in which recombination/repair processes (hRAD genes) play an important role. These coupled processes (duality in unity), to our view, underline tumor resistance by apoptotic inhibition and mutation fixation in normal cells exposed to anti-tumor agents. (C) 2003 Elsevier Ltd. All rights reserved.
T2  - Medical Hypotheses
T1  - Apoptotic versus genotoxic potential of antimtumor agents: a concept of duality in unity
VL  - 61
IS  - 5-6
SP  - 643
EP  - 650
DO  - 10.1016/S0306-9877(03)00266-4
ER  - 

@article{
author = "Vladan, B and Milićević, Zorka T. and Biljana, PS and Nedeljkovic-Kurepa, A",
year = "2003",
abstract = "Recent advances in anti-tumor therapy have raised a problem of secondary tumors and tumor resistance. Secondary tumors induced by chemotherapeutic agents as a consequence of primary therapy have poor prognostic outcome. Many new insights into molecular controls of cell cycle progression of normal and cancer cells can provide a useful framework in order to identify potential targets for anti-tumor therapies. One of the most promising strategies is the possibility to modulate apoptosis induced by anti-tumor agents. Cancer cell survival after chemotherapy will depend on specific checkpoints and/or repair pathways that have been lost, leading either to greater susceptibility to anti-tumor agents when the repair of damage is most important for survival or to greater resistance when the apoptotic response is more important. We have proposed a hypothesis that views survival and apoptotic processes (duality) in normal and tumor cells as genetically coupled (unity). We introduce, through a theoretical background, a new pathway of apoptotic inhibition. The proposed process of apoptotic inhibition is induced by mutation fixation in which recombination/repair processes (hRAD genes) play an important role. These coupled processes (duality in unity), to our view, underline tumor resistance by apoptotic inhibition and mutation fixation in normal cells exposed to anti-tumor agents. (C) 2003 Elsevier Ltd. All rights reserved.",
journal = "Medical Hypotheses",
title = "Apoptotic versus genotoxic potential of antimtumor agents: a concept of duality in unity",
volume = "61",
number = "5-6",
pages = "643-650",
doi = "10.1016/S0306-9877(03)00266-4"
}

Vladan, B., Milićević, Z. T., Biljana, P.,& Nedeljkovic-Kurepa, A.. (2003). Apoptotic versus genotoxic potential of antimtumor agents: a concept of duality in unity. in Medical Hypotheses, 61(5-6), 643-650.
https://doi.org/10.1016/S0306-9877(03)00266-4

Vladan B, Milićević ZT, Biljana P, Nedeljkovic-Kurepa A. Apoptotic versus genotoxic potential of antimtumor agents: a concept of duality in unity. in Medical Hypotheses. 2003;61(5-6):643-650.
doi:10.1016/S0306-9877(03)00266-4 .

Vladan, B, Milićević, Zorka T., Biljana, PS, Nedeljkovic-Kurepa, A, "Apoptotic versus genotoxic potential of antimtumor agents: a concept of duality in unity" in Medical Hypotheses, 61, no. 5-6 (2003):643-650,
https://doi.org/10.1016/S0306-9877(03)00266-4 . .

TY  - JOUR
AU  - Todorović, Vera
AU  - Janić, B.
AU  - Koko, Vesna
AU  - Micev, Marjan T.
AU  - Nikolić, Judith Anna
AU  - Ratković, Marija
AU  - Leposavić, Gordana
AU  - Janković, Teodora
AU  - Knežević-Ušaj, Slavica
AU  - Milićević, Zorka T.
PY  - 1996
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1990
AB  - Background/Aims: In this study, we present radioimmunoassay data describing the concentration of Vasoactive Intestinal Polypeptide (VIP) in both plasma and colonic biopsies, as well as immunostaining of VIPergic innervation in mucosal biopsies of normal subjects and patients with ulcerative colitis (UC). Patients and Methods: Thirty three patients with UC and 17 healthy subjects were investigated. All UC patients suffered from active disease. Fasting circulating levels of VIP in plasma as well as tissue concentrations were measured by radioimmunoassay. For the immunohistochemistry, polyclonal antibody against VIP and the streptavidin-biotin peroxidase complex technique were carried out. Results: Overall plasma VIP concentrations in the UC patients were similar to those in the controls. Significantly decreased concentrations of VIP were found in UC of rectum compared to the normal tissue. However, both plasma VIP values and tissue concentrations were found to be significantly lower in patients expressing minimal or mild active disease according to clinical activity index (AI) and histological activity index (HAI), but marked increase of plasma VIP was clear in UC patients with moderate or severe AI and HAI. There was a trend towards increased tissue concentrations of VIP in the group of patients with moderate or severe AI and HAI. Our immunohistochemical analysis of VIP fibers and nerve cell bodies revealed consistently weaker VIP-immunoreactivity in the rectum in UC patients with minimal or mild HAI. Simultaneously, in the rectal biopsies from UC patients with moderate and severe disease, the fibers in the lamina propria and ganglion cells in the submucous plexus were markedly increased in density and in degree of immunostaining. Very strong immunoreactivity was also found in inflammatory cells of the lamina propria as well as in the epithelial layer of the biopsies from UC patients with obvious disease. Conclusions: Our study shows clearly the heterogeneity in the response of VIP plasma level as well as rectum concentration and distribution in UC patients at different stages of the active disease. The possible role of VIP in the VIP in the colon suggests that further studies of the alterations of this gut peptide may be useful in the understanding of UC pathophysiology.
T2  - Hepato-gastroenterology
T1  - Colonic Vasoactive Intestinal Polypeptide (VIP) in ulcerative colitis - A radioimmunoassay and immunohistochemical study
VL  - 43
IS  - 9
SP  - 483
EP  - 488
UR  - https://hdl.handle.net/21.15107/rcub_vinar_1990
ER  - 

@article{
author = "Todorović, Vera and Janić, B. and Koko, Vesna and Micev, Marjan T. and Nikolić, Judith Anna and Ratković, Marija and Leposavić, Gordana and Janković, Teodora and Knežević-Ušaj, Slavica and Milićević, Zorka T.",
year = "1996",
abstract = "Background/Aims: In this study, we present radioimmunoassay data describing the concentration of Vasoactive Intestinal Polypeptide (VIP) in both plasma and colonic biopsies, as well as immunostaining of VIPergic innervation in mucosal biopsies of normal subjects and patients with ulcerative colitis (UC). Patients and Methods: Thirty three patients with UC and 17 healthy subjects were investigated. All UC patients suffered from active disease. Fasting circulating levels of VIP in plasma as well as tissue concentrations were measured by radioimmunoassay. For the immunohistochemistry, polyclonal antibody against VIP and the streptavidin-biotin peroxidase complex technique were carried out. Results: Overall plasma VIP concentrations in the UC patients were similar to those in the controls. Significantly decreased concentrations of VIP were found in UC of rectum compared to the normal tissue. However, both plasma VIP values and tissue concentrations were found to be significantly lower in patients expressing minimal or mild active disease according to clinical activity index (AI) and histological activity index (HAI), but marked increase of plasma VIP was clear in UC patients with moderate or severe AI and HAI. There was a trend towards increased tissue concentrations of VIP in the group of patients with moderate or severe AI and HAI. Our immunohistochemical analysis of VIP fibers and nerve cell bodies revealed consistently weaker VIP-immunoreactivity in the rectum in UC patients with minimal or mild HAI. Simultaneously, in the rectal biopsies from UC patients with moderate and severe disease, the fibers in the lamina propria and ganglion cells in the submucous plexus were markedly increased in density and in degree of immunostaining. Very strong immunoreactivity was also found in inflammatory cells of the lamina propria as well as in the epithelial layer of the biopsies from UC patients with obvious disease. Conclusions: Our study shows clearly the heterogeneity in the response of VIP plasma level as well as rectum concentration and distribution in UC patients at different stages of the active disease. The possible role of VIP in the VIP in the colon suggests that further studies of the alterations of this gut peptide may be useful in the understanding of UC pathophysiology.",
journal = "Hepato-gastroenterology",
title = "Colonic Vasoactive Intestinal Polypeptide (VIP) in ulcerative colitis - A radioimmunoassay and immunohistochemical study",
volume = "43",
number = "9",
pages = "483-488",
url = "https://hdl.handle.net/21.15107/rcub_vinar_1990"
}

Todorović, V., Janić, B., Koko, V., Micev, M. T., Nikolić, J. A., Ratković, M., Leposavić, G., Janković, T., Knežević-Ušaj, S.,& Milićević, Z. T.. (1996). Colonic Vasoactive Intestinal Polypeptide (VIP) in ulcerative colitis - A radioimmunoassay and immunohistochemical study. in Hepato-gastroenterology, 43(9), 483-488.
https://hdl.handle.net/21.15107/rcub_vinar_1990

Todorović V, Janić B, Koko V, Micev MT, Nikolić JA, Ratković M, Leposavić G, Janković T, Knežević-Ušaj S, Milićević ZT. Colonic Vasoactive Intestinal Polypeptide (VIP) in ulcerative colitis - A radioimmunoassay and immunohistochemical study. in Hepato-gastroenterology. 1996;43(9):483-488.
https://hdl.handle.net/21.15107/rcub_vinar_1990 .

Todorović, Vera, Janić, B., Koko, Vesna, Micev, Marjan T., Nikolić, Judith Anna, Ratković, Marija, Leposavić, Gordana, Janković, Teodora, Knežević-Ušaj, Slavica, Milićević, Zorka T., "Colonic Vasoactive Intestinal Polypeptide (VIP) in ulcerative colitis - A radioimmunoassay and immunohistochemical study" in Hepato-gastroenterology, 43, no. 9 (1996):483-488,
https://hdl.handle.net/21.15107/rcub_vinar_1990 .