TY  - JOUR
AU  - Vučićević, Jelica
AU  - Srdić-Rajić, Tatjana
AU  - Pieroni, Marco
AU  - Laurila, Jonne M. M.
AU  - Perović, Vladimir R.
AU  - Tassini, Sabrina
AU  - Azzali, Elisa
AU  - Costantino, Gabriele
AU  - Glišić, Sanja
AU  - Agbaba, Danica
AU  - Scheinin, Mika
AU  - Nikolić, Katarina M.
AU  - Radi, Marco
AU  - Veljković, Nevena V.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1110
AB  - The clonidine-like central antihypertensive agent rilmenidine, which has high affinity for I-1-type imidazoline receptors (I-1-IR) was recently found to have cytotoxic effects on cultured cancer cell lines. However, due to its pharmacological effects resulting also from alpha(2)-adrenoceptor activation, rilmenidine cannot be considered a suitable anticancer drug candidate. Here, we report the identification of novel rilmenidine- derived compounds with anticancer potential and devoid of alpha(2)-adrenoceptor effects by means of ligand-and structure-based drug design approaches. Starting from a large virtual library, eleven compounds were selected, synthesized and submitted to biological evaluation. The most active compound 5 exhibited a cytotoxic profile similar to that of rilmenidine, but without appreciable affinity to alpha(2)-adrenoceptors. In addition, compound 5 significantly enhanced the apoptotic response to doxorubicin, and may thus represent an important tool for the development of better adjuvant chemotherapeutic strategies for doxorubicin-insensitive cancers. (C) 2016 Elsevier Ltd. All rights reserved.
T2  - Bioorganic and Medicinal Chemistry
T1  - A combined ligand- and structure-based approach for the identification of rilmenidine-derived compounds which synergize the antitumor effects of doxorubicin
VL  - 24
IS  - 14
SP  - 3174
EP  - 3183
DO  - 10.1016/j.bmc.2016.05.043
ER  - 

@article{
author = "Vučićević, Jelica and Srdić-Rajić, Tatjana and Pieroni, Marco and Laurila, Jonne M. M. and Perović, Vladimir R. and Tassini, Sabrina and Azzali, Elisa and Costantino, Gabriele and Glišić, Sanja and Agbaba, Danica and Scheinin, Mika and Nikolić, Katarina M. and Radi, Marco and Veljković, Nevena V.",
year = "2016",
abstract = "The clonidine-like central antihypertensive agent rilmenidine, which has high affinity for I-1-type imidazoline receptors (I-1-IR) was recently found to have cytotoxic effects on cultured cancer cell lines. However, due to its pharmacological effects resulting also from alpha(2)-adrenoceptor activation, rilmenidine cannot be considered a suitable anticancer drug candidate. Here, we report the identification of novel rilmenidine- derived compounds with anticancer potential and devoid of alpha(2)-adrenoceptor effects by means of ligand-and structure-based drug design approaches. Starting from a large virtual library, eleven compounds were selected, synthesized and submitted to biological evaluation. The most active compound 5 exhibited a cytotoxic profile similar to that of rilmenidine, but without appreciable affinity to alpha(2)-adrenoceptors. In addition, compound 5 significantly enhanced the apoptotic response to doxorubicin, and may thus represent an important tool for the development of better adjuvant chemotherapeutic strategies for doxorubicin-insensitive cancers. (C) 2016 Elsevier Ltd. All rights reserved.",
journal = "Bioorganic and Medicinal Chemistry",
title = "A combined ligand- and structure-based approach for the identification of rilmenidine-derived compounds which synergize the antitumor effects of doxorubicin",
volume = "24",
number = "14",
pages = "3174-3183",
doi = "10.1016/j.bmc.2016.05.043"
}

Vučićević, J., Srdić-Rajić, T., Pieroni, M., Laurila, J. M. M., Perović, V. R., Tassini, S., Azzali, E., Costantino, G., Glišić, S., Agbaba, D., Scheinin, M., Nikolić, K. M., Radi, M.,& Veljković, N. V.. (2016). A combined ligand- and structure-based approach for the identification of rilmenidine-derived compounds which synergize the antitumor effects of doxorubicin. in Bioorganic and Medicinal Chemistry, 24(14), 3174-3183.
https://doi.org/10.1016/j.bmc.2016.05.043

Vučićević J, Srdić-Rajić T, Pieroni M, Laurila JMM, Perović VR, Tassini S, Azzali E, Costantino G, Glišić S, Agbaba D, Scheinin M, Nikolić KM, Radi M, Veljković NV. A combined ligand- and structure-based approach for the identification of rilmenidine-derived compounds which synergize the antitumor effects of doxorubicin. in Bioorganic and Medicinal Chemistry. 2016;24(14):3174-3183.
doi:10.1016/j.bmc.2016.05.043 .

Vučićević, Jelica, Srdić-Rajić, Tatjana, Pieroni, Marco, Laurila, Jonne M. M., Perović, Vladimir R., Tassini, Sabrina, Azzali, Elisa, Costantino, Gabriele, Glišić, Sanja, Agbaba, Danica, Scheinin, Mika, Nikolić, Katarina M., Radi, Marco, Veljković, Nevena V., "A combined ligand- and structure-based approach for the identification of rilmenidine-derived compounds which synergize the antitumor effects of doxorubicin" in Bioorganic and Medicinal Chemistry, 24, no. 14 (2016):3174-3183,
https://doi.org/10.1016/j.bmc.2016.05.043 . .

TY  - JOUR
AU  - Nikolić, Katarina M.
AU  - Veljković, Nevena V.
AU  - Gemović, Branislava S.
AU  - Srdić-Rajić, Tatjana
AU  - Agbaba, Danica
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5398
AB  - The group of imidazoline-1 receptors (I-1-IR) agonists encompasses drugs are currently used in treatment of high blood pressure and hyperglycemia. The I-1-IR protein structures have not been determined yet, but Nischarin protein that binds numerous imidazoline ligands inducing initiation of various cell-signaling cascades, including apoptosis, is identified as strong I-1-IR candidate. In this study we examined apoptotic activity of rilmenidine (potent I-1-IR agonist), moxonidine (moderate I-1-IR agonist), and efaroxan (I-1-IR partial agonist) on cancer cell line (K562) expressing Nischarin. The Nischarine domains mapping was performed by use of the Informational Spectrum Method (ISM). The 3D-Quantitative Structure-Activity Relationship (3D-QSAR) and virtual docking studies of 29 I-1-IR ligands (agonists, partial agonists, and antagonists) were carried out on I-1-IR receptors binding affinities. The 3D-QSAR study defined 3D-pharmacophore models for I-1-IR agonistic and I-1-IR antagonistic activity and created regression model for prediction of I-1-IR activity of novel compounds. The 3D-QSAR models were applied for design and evaluation of novel I-1-IR agonists and I-1-IR antagonists. The most promising I-1-IR ligands with enhanced activities than parent compounds were proposed for synthesis. The results of 3D-QSAR, ISM, and virtual docking studies were in perfect agreement and allowed precise definition of binding mode of I-1-IR agonists (Arg 758, Arg 866, Val 981, and Glu 1057) and significantly different binding modes of I-1-IR antagonists or partial I-1-IR agonists. The performed theoretical study provides reliable system for evaluation of I-1-IR agonistic and I-1-IR antagonistic activity of novel I-1-IR ligands, as drug candidates with anticancer activities.
T2  - Combinatorial Chemistry and High Throughput Screening
T1  - Imidazoline-1 Receptor Ligands as Apoptotic Agents: Pharmacophore Modeling and Virtual Docking Study
VL  - 16
IS  - 4
SP  - 298
EP  - 319
DO  - 10.2174/1386207311316040004
ER  - 

@article{
author = "Nikolić, Katarina M. and Veljković, Nevena V. and Gemović, Branislava S. and Srdić-Rajić, Tatjana and Agbaba, Danica",
year = "2013",
abstract = "The group of imidazoline-1 receptors (I-1-IR) agonists encompasses drugs are currently used in treatment of high blood pressure and hyperglycemia. The I-1-IR protein structures have not been determined yet, but Nischarin protein that binds numerous imidazoline ligands inducing initiation of various cell-signaling cascades, including apoptosis, is identified as strong I-1-IR candidate. In this study we examined apoptotic activity of rilmenidine (potent I-1-IR agonist), moxonidine (moderate I-1-IR agonist), and efaroxan (I-1-IR partial agonist) on cancer cell line (K562) expressing Nischarin. The Nischarine domains mapping was performed by use of the Informational Spectrum Method (ISM). The 3D-Quantitative Structure-Activity Relationship (3D-QSAR) and virtual docking studies of 29 I-1-IR ligands (agonists, partial agonists, and antagonists) were carried out on I-1-IR receptors binding affinities. The 3D-QSAR study defined 3D-pharmacophore models for I-1-IR agonistic and I-1-IR antagonistic activity and created regression model for prediction of I-1-IR activity of novel compounds. The 3D-QSAR models were applied for design and evaluation of novel I-1-IR agonists and I-1-IR antagonists. The most promising I-1-IR ligands with enhanced activities than parent compounds were proposed for synthesis. The results of 3D-QSAR, ISM, and virtual docking studies were in perfect agreement and allowed precise definition of binding mode of I-1-IR agonists (Arg 758, Arg 866, Val 981, and Glu 1057) and significantly different binding modes of I-1-IR antagonists or partial I-1-IR agonists. The performed theoretical study provides reliable system for evaluation of I-1-IR agonistic and I-1-IR antagonistic activity of novel I-1-IR ligands, as drug candidates with anticancer activities.",
journal = "Combinatorial Chemistry and High Throughput Screening",
title = "Imidazoline-1 Receptor Ligands as Apoptotic Agents: Pharmacophore Modeling and Virtual Docking Study",
volume = "16",
number = "4",
pages = "298-319",
doi = "10.2174/1386207311316040004"
}

Nikolić, K. M., Veljković, N. V., Gemović, B. S., Srdić-Rajić, T.,& Agbaba, D.. (2013). Imidazoline-1 Receptor Ligands as Apoptotic Agents: Pharmacophore Modeling and Virtual Docking Study. in Combinatorial Chemistry and High Throughput Screening, 16(4), 298-319.
https://doi.org/10.2174/1386207311316040004

Nikolić KM, Veljković NV, Gemović BS, Srdić-Rajić T, Agbaba D. Imidazoline-1 Receptor Ligands as Apoptotic Agents: Pharmacophore Modeling and Virtual Docking Study. in Combinatorial Chemistry and High Throughput Screening. 2013;16(4):298-319.
doi:10.2174/1386207311316040004 .

Nikolić, Katarina M., Veljković, Nevena V., Gemović, Branislava S., Srdić-Rajić, Tatjana, Agbaba, Danica, "Imidazoline-1 Receptor Ligands as Apoptotic Agents: Pharmacophore Modeling and Virtual Docking Study" in Combinatorial Chemistry and High Throughput Screening, 16, no. 4 (2013):298-319,
https://doi.org/10.2174/1386207311316040004 . .

TY  - JOUR
AU  - Marinković, Valentina D.
AU  - Agbaba, Danica
AU  - Karjikovic-Rajic, K
AU  - Vladimirov, Sote
AU  - Nedeljković, Jovan
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6377
AB  - The photochemical degradation of solid-state nisoldipine, 1,4-dihydropyridine calcium antagonist, was investigated under daylight and UV light conditions. Degradation products were identified by using the retention times of corresponding standards and quantified by high-performance liquid chromatographic method. The daylight illumination induced appearance of nitrosophenylpyridine, while formation of second degradation product, nitrophenylpyridine, was observed only upon UV light illumination. The photodegradation kinetics of solid-state nisoldipine under daylight and UV light illumination belongs to class of zero-order reactions. The rate constants of disappearance of nisoldipine upon illumination were determined for raw material as well as pharmaceuticals (tablets, film-tablets and capsules). (C) 2003 Elsevier Science B.V. All rights reserved.
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Photochemical degradation of solid-state nisoldipine monitored by HPLC
VL  - 32
IS  - 4-5
SP  - 929
EP  - 935
DO  - 10.1016/S0731-7085(03)00194-8
ER  - 

@article{
author = "Marinković, Valentina D. and Agbaba, Danica and Karjikovic-Rajic, K and Vladimirov, Sote and Nedeljković, Jovan",
year = "2003",
abstract = "The photochemical degradation of solid-state nisoldipine, 1,4-dihydropyridine calcium antagonist, was investigated under daylight and UV light conditions. Degradation products were identified by using the retention times of corresponding standards and quantified by high-performance liquid chromatographic method. The daylight illumination induced appearance of nitrosophenylpyridine, while formation of second degradation product, nitrophenylpyridine, was observed only upon UV light illumination. The photodegradation kinetics of solid-state nisoldipine under daylight and UV light illumination belongs to class of zero-order reactions. The rate constants of disappearance of nisoldipine upon illumination were determined for raw material as well as pharmaceuticals (tablets, film-tablets and capsules). (C) 2003 Elsevier Science B.V. All rights reserved.",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Photochemical degradation of solid-state nisoldipine monitored by HPLC",
volume = "32",
number = "4-5",
pages = "929-935",
doi = "10.1016/S0731-7085(03)00194-8"
}

Marinković, V. D., Agbaba, D., Karjikovic-Rajic, K., Vladimirov, S.,& Nedeljković, J.. (2003). Photochemical degradation of solid-state nisoldipine monitored by HPLC. in Journal of Pharmaceutical and Biomedical Analysis, 32(4-5), 929-935.
https://doi.org/10.1016/S0731-7085(03)00194-8

Marinković VD, Agbaba D, Karjikovic-Rajic K, Vladimirov S, Nedeljković J. Photochemical degradation of solid-state nisoldipine monitored by HPLC. in Journal of Pharmaceutical and Biomedical Analysis. 2003;32(4-5):929-935.
doi:10.1016/S0731-7085(03)00194-8 .

Marinković, Valentina D., Agbaba, Danica, Karjikovic-Rajic, K, Vladimirov, Sote, Nedeljković, Jovan, "Photochemical degradation of solid-state nisoldipine monitored by HPLC" in Journal of Pharmaceutical and Biomedical Analysis, 32, no. 4-5 (2003):929-935,
https://doi.org/10.1016/S0731-7085(03)00194-8 . .

TY  - JOUR
AU  - Marinkovic, V
AU  - Agbaba, Danica
AU  - Karljiković-Rajić, Katarina
AU  - Čomor, Jožef J.
AU  - Zivanov-Stakic, D
PY  - 2000
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2337
AB  - The photodecomposition of nisoldipine ((+/-)3-isobutyl-5-methyl-1,4-dihydro-2,6-dimethyl-4-(2-nitrophenyl)-pyridine-3,5-dicarboxylate), whereby its 4-(2-nitrosophenyl) pyridine analogue is obtained as the photolytic product, was investigated under daylight exposure by means of UV derivative spectrophotometry. The optimal instrumental parameters (120 nm/min scan speed: 2 nm slit width; Delta lambda = 10 nm and 5 s response time) for analogue derivative spectra were established for amplitudes D-1(285) and D-2(291) (measured to the baseline) of the nitroso analogue assay, as well as for D-1(386) of the parent compound-nisoldipine assay. Using the first-order derivative spectrum, the minimum detectable amount of nitroso analogue in the presence of nisoldipine was equivalent to an impurity level of 5% and by the second-order derivative spectrum, the determination limit was equivalent to an impurity level of 2%. The degradation of nisoldipine followed within 30 days and the calculated maximal degradation rate was 1.6% per day for nisoldipine raw material, but significantly lower values of 0.19 and 0.15% per day were obtained for Nisoldin(R) tablets (10 and 5 mg, respectively). (C) 2000 Elsevier Science S.A. All rights reserved.
T2  - Il Farmaco
T1  - UV derivative spectrophotometric study of the photochemical degradation of nisoldipine
VL  - 55
IS  - 2
SP  - 128
EP  - 133
DO  - 10.1016/S0014-827X(00)00004-5
ER  - 

@article{
author = "Marinkovic, V and Agbaba, Danica and Karljiković-Rajić, Katarina and Čomor, Jožef J. and Zivanov-Stakic, D",
year = "2000",
abstract = "The photodecomposition of nisoldipine ((+/-)3-isobutyl-5-methyl-1,4-dihydro-2,6-dimethyl-4-(2-nitrophenyl)-pyridine-3,5-dicarboxylate), whereby its 4-(2-nitrosophenyl) pyridine analogue is obtained as the photolytic product, was investigated under daylight exposure by means of UV derivative spectrophotometry. The optimal instrumental parameters (120 nm/min scan speed: 2 nm slit width; Delta lambda = 10 nm and 5 s response time) for analogue derivative spectra were established for amplitudes D-1(285) and D-2(291) (measured to the baseline) of the nitroso analogue assay, as well as for D-1(386) of the parent compound-nisoldipine assay. Using the first-order derivative spectrum, the minimum detectable amount of nitroso analogue in the presence of nisoldipine was equivalent to an impurity level of 5% and by the second-order derivative spectrum, the determination limit was equivalent to an impurity level of 2%. The degradation of nisoldipine followed within 30 days and the calculated maximal degradation rate was 1.6% per day for nisoldipine raw material, but significantly lower values of 0.19 and 0.15% per day were obtained for Nisoldin(R) tablets (10 and 5 mg, respectively). (C) 2000 Elsevier Science S.A. All rights reserved.",
journal = "Il Farmaco",
title = "UV derivative spectrophotometric study of the photochemical degradation of nisoldipine",
volume = "55",
number = "2",
pages = "128-133",
doi = "10.1016/S0014-827X(00)00004-5"
}

Marinkovic, V., Agbaba, D., Karljiković-Rajić, K., Čomor, J. J.,& Zivanov-Stakic, D.. (2000). UV derivative spectrophotometric study of the photochemical degradation of nisoldipine. in Il Farmaco, 55(2), 128-133.
https://doi.org/10.1016/S0014-827X(00)00004-5

Marinkovic V, Agbaba D, Karljiković-Rajić K, Čomor JJ, Zivanov-Stakic D. UV derivative spectrophotometric study of the photochemical degradation of nisoldipine. in Il Farmaco. 2000;55(2):128-133.
doi:10.1016/S0014-827X(00)00004-5 .

Marinkovic, V, Agbaba, Danica, Karljiković-Rajić, Katarina, Čomor, Jožef J., Zivanov-Stakic, D, "UV derivative spectrophotometric study of the photochemical degradation of nisoldipine" in Il Farmaco, 55, no. 2 (2000):128-133,
https://doi.org/10.1016/S0014-827X(00)00004-5 . .

TY  - JOUR
AU  - Marinković, Valentina D.
AU  - Milojković, Smiljana S.
AU  - Nedeljković, Jovan
AU  - Čomor, Jožef J.
AU  - Agbaba, Danica
AU  - Živanov-Stakić, Dobrila
PY  - 1997
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2099
AB  - A straightforward quantitative method for gas chromatography-mass spectrometry determination of isosorbide 5-mononitrate (ISSMN) and its related impurities such as isosorbide (IS), isosorbide diacetate (ISDA) and isosorbide 2-acetate-5-nitrate (IS2A5N) in raw materials as well as in dosage formulations is developed. The recovery of these materials was found to be 100.4 +/- 2.4, 99.3 +/- 4.7, 97.8 +/- 5.2 and 100.1 +/- 3.1%, while the detection limits were 27.2, 1.26, 1.02 and 0.78 mu g in dosage formulations for ISSMN, ISDA, IS2A5N, and IS, respectively. The applicability of the method was tested by analysing three different formulations of ISSMN. (C) 1997 Elsevier Science B.V.
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Gas chromatography mass spectrometry determination of isosorbide 5-mononitrate and related impurities in raw materials and dosage formulations
VL  - 16
IS  - 3
SP  - 425
EP  - 429
DO  - 10.1016/S0731-7085(97)00079-4
ER  - 

@article{
author = "Marinković, Valentina D. and Milojković, Smiljana S. and Nedeljković, Jovan and Čomor, Jožef J. and Agbaba, Danica and Živanov-Stakić, Dobrila",
year = "1997",
abstract = "A straightforward quantitative method for gas chromatography-mass spectrometry determination of isosorbide 5-mononitrate (ISSMN) and its related impurities such as isosorbide (IS), isosorbide diacetate (ISDA) and isosorbide 2-acetate-5-nitrate (IS2A5N) in raw materials as well as in dosage formulations is developed. The recovery of these materials was found to be 100.4 +/- 2.4, 99.3 +/- 4.7, 97.8 +/- 5.2 and 100.1 +/- 3.1%, while the detection limits were 27.2, 1.26, 1.02 and 0.78 mu g in dosage formulations for ISSMN, ISDA, IS2A5N, and IS, respectively. The applicability of the method was tested by analysing three different formulations of ISSMN. (C) 1997 Elsevier Science B.V.",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Gas chromatography mass spectrometry determination of isosorbide 5-mononitrate and related impurities in raw materials and dosage formulations",
volume = "16",
number = "3",
pages = "425-429",
doi = "10.1016/S0731-7085(97)00079-4"
}

Marinković, V. D., Milojković, S. S., Nedeljković, J., Čomor, J. J., Agbaba, D.,& Živanov-Stakić, D.. (1997). Gas chromatography mass spectrometry determination of isosorbide 5-mononitrate and related impurities in raw materials and dosage formulations. in Journal of Pharmaceutical and Biomedical Analysis, 16(3), 425-429.
https://doi.org/10.1016/S0731-7085(97)00079-4

Marinković VD, Milojković SS, Nedeljković J, Čomor JJ, Agbaba D, Živanov-Stakić D. Gas chromatography mass spectrometry determination of isosorbide 5-mononitrate and related impurities in raw materials and dosage formulations. in Journal of Pharmaceutical and Biomedical Analysis. 1997;16(3):425-429.
doi:10.1016/S0731-7085(97)00079-4 .

Marinković, Valentina D., Milojković, Smiljana S., Nedeljković, Jovan, Čomor, Jožef J., Agbaba, Danica, Živanov-Stakić, Dobrila, "Gas chromatography mass spectrometry determination of isosorbide 5-mononitrate and related impurities in raw materials and dosage formulations" in Journal of Pharmaceutical and Biomedical Analysis, 16, no. 3 (1997):425-429,
https://doi.org/10.1016/S0731-7085(97)00079-4 . .

TY  - JOUR
AU  - Vladimirov, Sote
AU  - Čudina, Olivera
AU  - Agbaba, Danica
AU  - Jovanovic, M
AU  - Živanov-Stakić, Dobrila
PY  - 1996
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7217
AB  - The proposed method is based on coloured hydrazone formation with 1,4-dihydrazinophthalazine as a reagent. Heating at 85 degrees C for 2 h was found necessary to ensure optimal hydrazone formation in the presence of hydrochloric acid. The yellow hydrazone product has an absorption maximum at 380 nm. A linear relationship between absorbance and concentration was established in the concentration range 3.19 x 10(-6)-3.19 x 10(-5) mol l(-1) (the regression equation was y = 0.013 167 3 + 0.019 025 9x; correlation coefficient r = 0.9991; n = 6). The detection limit was 1.2 mu g ml(-1) (molar absorptivity found was 1.97 x 10(4) l mol(-1) cm(-1)). The reliability of the proposed method was checked at three different concentrations; the relative standard deviation (RSD) varied from 1.03 to 2.01%. The described method applied to the determination of desoximetasone in ointment gave precise and reproducible results; the recovery was 98.55% with RSD = 2.40% (n = 10).
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Spectrophotometric determination of desoximetasone in ointment using 1,4-dihydrazinophthalazine
VL  - 14
IS  - 8-10
SP  - 947
EP  - 950
DO  - 10.1016/0731-7085(95)01681-3
ER  - 

@article{
author = "Vladimirov, Sote and Čudina, Olivera and Agbaba, Danica and Jovanovic, M and Živanov-Stakić, Dobrila",
year = "1996",
abstract = "The proposed method is based on coloured hydrazone formation with 1,4-dihydrazinophthalazine as a reagent. Heating at 85 degrees C for 2 h was found necessary to ensure optimal hydrazone formation in the presence of hydrochloric acid. The yellow hydrazone product has an absorption maximum at 380 nm. A linear relationship between absorbance and concentration was established in the concentration range 3.19 x 10(-6)-3.19 x 10(-5) mol l(-1) (the regression equation was y = 0.013 167 3 + 0.019 025 9x; correlation coefficient r = 0.9991; n = 6). The detection limit was 1.2 mu g ml(-1) (molar absorptivity found was 1.97 x 10(4) l mol(-1) cm(-1)). The reliability of the proposed method was checked at three different concentrations; the relative standard deviation (RSD) varied from 1.03 to 2.01%. The described method applied to the determination of desoximetasone in ointment gave precise and reproducible results; the recovery was 98.55% with RSD = 2.40% (n = 10).",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Spectrophotometric determination of desoximetasone in ointment using 1,4-dihydrazinophthalazine",
volume = "14",
number = "8-10",
pages = "947-950",
doi = "10.1016/0731-7085(95)01681-3"
}

Vladimirov, S., Čudina, O., Agbaba, D., Jovanovic, M.,& Živanov-Stakić, D.. (1996). Spectrophotometric determination of desoximetasone in ointment using 1,4-dihydrazinophthalazine. in Journal of Pharmaceutical and Biomedical Analysis, 14(8-10), 947-950.
https://doi.org/10.1016/0731-7085(95)01681-3

Vladimirov S, Čudina O, Agbaba D, Jovanovic M, Živanov-Stakić D. Spectrophotometric determination of desoximetasone in ointment using 1,4-dihydrazinophthalazine. in Journal of Pharmaceutical and Biomedical Analysis. 1996;14(8-10):947-950.
doi:10.1016/0731-7085(95)01681-3 .

Vladimirov, Sote, Čudina, Olivera, Agbaba, Danica, Jovanovic, M, Živanov-Stakić, Dobrila, "Spectrophotometric determination of desoximetasone in ointment using 1,4-dihydrazinophthalazine" in Journal of Pharmaceutical and Biomedical Analysis, 14, no. 8-10 (1996):947-950,
https://doi.org/10.1016/0731-7085(95)01681-3 . .