TY  - JOUR
AU  - Bergman, Naomi
AU  - Moraes, Karen C. M.
AU  - Anderson, John R.
AU  - Zarić, Božidarka
AU  - Kambach, Christian
AU  - Schneider, Robert J.
AU  - Wilusz, Carol J.
AU  - Wilusz, Jeffrey
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3277
AB  - Many orthopoxvirus messenger RNAs have an unusual nontemplated poly( A) tract of 5 to 40 residues at the 5 end. The precise function of this feature is unknown. Here we show that 5 poly( A) tracts are able to repress RNA decay by inhibiting 3 - to- 5 exonucleases as well as decapping of RNA substrates. UV cross- linking analysis demonstrated that the Lsm complex associates with the 5 poly( A) tract. Furthermore, recombinant Lsm1 - 7 complex specifically binds 5 poly( A) tracts 10 to 21 nucleotides in length, consistent with the length of 5 poly( A) required for stabilization. Knockdown of Lsm1 abrogates RNA stabilization by the 5 poly( A) tract. We propose that the Lsm complex simultaneously binds the 3 and 5 ends of these unusual messenger RNAs and thereby prevents 3 - to-5 decay. The implications of this phenomenon for cellular mRNA decay are discussed.
T2  - Nature Structural and Molecular Biology
T1  - Lsm proteins bind and stabilize RNAs containing 5 poly(A) tracts
VL  - 14
IS  - 9
SP  - 824
EP  - 831
DO  - 10.1038/nsmb1287
ER  - 

@article{
author = "Bergman, Naomi and Moraes, Karen C. M. and Anderson, John R. and Zarić, Božidarka and Kambach, Christian and Schneider, Robert J. and Wilusz, Carol J. and Wilusz, Jeffrey",
year = "2007",
abstract = "Many orthopoxvirus messenger RNAs have an unusual nontemplated poly( A) tract of 5 to 40 residues at the 5 end. The precise function of this feature is unknown. Here we show that 5 poly( A) tracts are able to repress RNA decay by inhibiting 3 - to- 5 exonucleases as well as decapping of RNA substrates. UV cross- linking analysis demonstrated that the Lsm complex associates with the 5 poly( A) tract. Furthermore, recombinant Lsm1 - 7 complex specifically binds 5 poly( A) tracts 10 to 21 nucleotides in length, consistent with the length of 5 poly( A) required for stabilization. Knockdown of Lsm1 abrogates RNA stabilization by the 5 poly( A) tract. We propose that the Lsm complex simultaneously binds the 3 and 5 ends of these unusual messenger RNAs and thereby prevents 3 - to-5 decay. The implications of this phenomenon for cellular mRNA decay are discussed.",
journal = "Nature Structural and Molecular Biology",
title = "Lsm proteins bind and stabilize RNAs containing 5 poly(A) tracts",
volume = "14",
number = "9",
pages = "824-831",
doi = "10.1038/nsmb1287"
}

Bergman, N., Moraes, K. C. M., Anderson, J. R., Zarić, B., Kambach, C., Schneider, R. J., Wilusz, C. J.,& Wilusz, J.. (2007). Lsm proteins bind and stabilize RNAs containing 5 poly(A) tracts. in Nature Structural and Molecular Biology, 14(9), 824-831.
https://doi.org/10.1038/nsmb1287

Bergman N, Moraes KCM, Anderson JR, Zarić B, Kambach C, Schneider RJ, Wilusz CJ, Wilusz J. Lsm proteins bind and stabilize RNAs containing 5 poly(A) tracts. in Nature Structural and Molecular Biology. 2007;14(9):824-831.
doi:10.1038/nsmb1287 .

Bergman, Naomi, Moraes, Karen C. M., Anderson, John R., Zarić, Božidarka, Kambach, Christian, Schneider, Robert J., Wilusz, Carol J., Wilusz, Jeffrey, "Lsm proteins bind and stabilize RNAs containing 5 poly(A) tracts" in Nature Structural and Molecular Biology, 14, no. 9 (2007):824-831,
https://doi.org/10.1038/nsmb1287 . .