TY  - JOUR
AU  - Tanić, Nikola
AU  - Brkić, G
AU  - Dimitrijević, Bogomir B.
AU  - Dedović-Tanić, Nasta
AU  - Gefen, N
AU  - Benharroch, D
AU  - Gopas, J
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3032
AB  - Background: Malignant melanoma resistance to chemotherapy remains a major limitation to treatment. Our aim was to identify genes associated with drug resistance, in order to better understand the molecular events underlying the drug-resistant phenotype. Materials and Methods: A human melanoma cell line and its drug-resistant variants obtained by selection with MNNG or 6-thioguanine were used. Alterations in gene expression were characterized by differential display reverse transcription-polymerase chain reaction (DDRT-PCR). Prominent mRNA fragments present in selected variants and not in the parental cells were identified and characterized by cloning and sequencing. Differential expression was confirmed by real-time RT-PCR. Results: Three functionally distinct transcriptional products were demonstrated: the chaperonin subunit TCP 1-zeta-6A (CCT6A), the hyaluronan receptor CD44 and LPPR-2, the lipid phosphate phosphatase-related protein type-2. Conclusion: Genes with altered expression were identified in drug-resistant variants. The identified molecules may provide new insights into the molecular basis for melanoma resistance to chemotherapy.
T2  - Anticancer Research
T1  - Identification of differentially expressed mRNA transcripts in drug-resistant versus parental human melanoma cell lines
VL  - 26
IS  - 3A
SP  - 2137
EP  - 2142
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3032
ER  - 

@article{
author = "Tanić, Nikola and Brkić, G and Dimitrijević, Bogomir B. and Dedović-Tanić, Nasta and Gefen, N and Benharroch, D and Gopas, J",
year = "2006",
abstract = "Background: Malignant melanoma resistance to chemotherapy remains a major limitation to treatment. Our aim was to identify genes associated with drug resistance, in order to better understand the molecular events underlying the drug-resistant phenotype. Materials and Methods: A human melanoma cell line and its drug-resistant variants obtained by selection with MNNG or 6-thioguanine were used. Alterations in gene expression were characterized by differential display reverse transcription-polymerase chain reaction (DDRT-PCR). Prominent mRNA fragments present in selected variants and not in the parental cells were identified and characterized by cloning and sequencing. Differential expression was confirmed by real-time RT-PCR. Results: Three functionally distinct transcriptional products were demonstrated: the chaperonin subunit TCP 1-zeta-6A (CCT6A), the hyaluronan receptor CD44 and LPPR-2, the lipid phosphate phosphatase-related protein type-2. Conclusion: Genes with altered expression were identified in drug-resistant variants. The identified molecules may provide new insights into the molecular basis for melanoma resistance to chemotherapy.",
journal = "Anticancer Research",
title = "Identification of differentially expressed mRNA transcripts in drug-resistant versus parental human melanoma cell lines",
volume = "26",
number = "3A",
pages = "2137-2142",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3032"
}

Tanić, N., Brkić, G., Dimitrijević, B. B., Dedović-Tanić, N., Gefen, N., Benharroch, D.,& Gopas, J.. (2006). Identification of differentially expressed mRNA transcripts in drug-resistant versus parental human melanoma cell lines. in Anticancer Research, 26(3A), 2137-2142.
https://hdl.handle.net/21.15107/rcub_vinar_3032

Tanić N, Brkić G, Dimitrijević BB, Dedović-Tanić N, Gefen N, Benharroch D, Gopas J. Identification of differentially expressed mRNA transcripts in drug-resistant versus parental human melanoma cell lines. in Anticancer Research. 2006;26(3A):2137-2142.
https://hdl.handle.net/21.15107/rcub_vinar_3032 .

Tanić, Nikola, Brkić, G, Dimitrijević, Bogomir B., Dedović-Tanić, Nasta, Gefen, N, Benharroch, D, Gopas, J, "Identification of differentially expressed mRNA transcripts in drug-resistant versus parental human melanoma cell lines" in Anticancer Research, 26, no. 3A (2006):2137-2142,
https://hdl.handle.net/21.15107/rcub_vinar_3032 .