Effects of Intracerebroventricularly (ICV) Injected Ghrelin on Cardiac Inducible Nitric Oxide Synthase Activity/Expression in Obese Rats
Само за регистроване кориснике
2015
Аутори
Sudar, EminaJovanović, Aleksandra
Misirkić-Marjanović, Maja
Vučićević, Ljubica
Janjetović, Kristina D.
Isenović, Esma R.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to examine the effects of ghrelin on regulation of cardiac inducible nitric oxide synthase (iNOS) activity/expression in high fat (HF), obese rats. For this study, male Wistar rats fed with HF diet (30 % fat) for 4 weeks were injected every 24 h for 5 days intracerebroventriculary (ICV) with ghrelin (0.3 nmol/5 mu l) or with an equal volume of phosphate buffered saline (PBS). Control rats were ICV injected with an equal volume of PBS. Glucose, insulin and nitric oxide (NO) concentrations were measured in serum, while arginase activity and citrulline concentrations were measured in heart lysate. Protein iNOS and regulatory subunit of nuclear factor-kappa B (NF kappa B-p65), phosphorylation of enzymes protein kinase B (Akt) at Ser(473), and extracellular signal-regulated kinases 1/2 (ERK1/2) at Tyr(202)/Tyr(204) were determined in heart lysate by Western blot. For gene expression of iNOS qRT-PCR was used. Results show significantly (p LT 0.01) higher serum NO pr...oduction in ghrelin treated HF rats compared with HF rats. Ghrelin significantly reduced citrulline concentration (p LT 0.05) and arginase activity (p LT 0.01) in HF rats. In ghrelin treated HF rats, gene and protein expression of iNOS and NF kappa B-p65 levels were significantly (p LT 0.05) increased compared with HF rats. Increased phosphorylation of Akt (p LT 0.01) and decreased (p LT 0.05) ERK1/2 phosphorylation were detected in HF ghrelin treated rats compared with HF rats hearts. Results from this study indicate that exogenous ghrelin induces expression and activity of cardiac iNOS via Akt phosphorylation followed by NF kappa B activation in HF rats.
Кључне речи:
ghrelin / heart / nitric oxide / nitric oxide synthase / protein kinase B / extracellular signal-regulated kinases 1/2Извор:
Experimental and Clinical Endocrinology and Diabetes, 2015, 123, 10, 581-588Издавач:
- Georg Thieme Verlag KG
Финансирање / пројекти:
- Хормонска регулација експресије и активности азот оксид синтазе и натријум-калијумове пумпе у експерименталним моделима инсулинске резистенције, дијабетеса и кардиоваскуларних поремећаја (RS-MESTD-Basic Research (BR or ON)-173033)
- Модулација сигналних путева који контролишу интрацелуларни енергетски баланс у терапији тумора и неуро-имуно-ендокриних поремећаја (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41025)
DOI: 10.1055/s-0035-1559758
ISSN: 0947-7349; 1439-3646
WoS: 000365626900002
Scopus: 2-s2.0-84948184682
Колекције
Институција/група
VinčaTY - JOUR AU - Sudar, Emina AU - Jovanović, Aleksandra AU - Misirkić-Marjanović, Maja AU - Vučićević, Ljubica AU - Janjetović, Kristina D. AU - Isenović, Esma R. PY - 2015 UR - https://vinar.vin.bg.ac.rs/handle/123456789/832 AB - The aim of this study was to examine the effects of ghrelin on regulation of cardiac inducible nitric oxide synthase (iNOS) activity/expression in high fat (HF), obese rats. For this study, male Wistar rats fed with HF diet (30 % fat) for 4 weeks were injected every 24 h for 5 days intracerebroventriculary (ICV) with ghrelin (0.3 nmol/5 mu l) or with an equal volume of phosphate buffered saline (PBS). Control rats were ICV injected with an equal volume of PBS. Glucose, insulin and nitric oxide (NO) concentrations were measured in serum, while arginase activity and citrulline concentrations were measured in heart lysate. Protein iNOS and regulatory subunit of nuclear factor-kappa B (NF kappa B-p65), phosphorylation of enzymes protein kinase B (Akt) at Ser(473), and extracellular signal-regulated kinases 1/2 (ERK1/2) at Tyr(202)/Tyr(204) were determined in heart lysate by Western blot. For gene expression of iNOS qRT-PCR was used. Results show significantly (p LT 0.01) higher serum NO production in ghrelin treated HF rats compared with HF rats. Ghrelin significantly reduced citrulline concentration (p LT 0.05) and arginase activity (p LT 0.01) in HF rats. In ghrelin treated HF rats, gene and protein expression of iNOS and NF kappa B-p65 levels were significantly (p LT 0.05) increased compared with HF rats. Increased phosphorylation of Akt (p LT 0.01) and decreased (p LT 0.05) ERK1/2 phosphorylation were detected in HF ghrelin treated rats compared with HF rats hearts. Results from this study indicate that exogenous ghrelin induces expression and activity of cardiac iNOS via Akt phosphorylation followed by NF kappa B activation in HF rats. PB - Georg Thieme Verlag KG T2 - Experimental and Clinical Endocrinology and Diabetes T1 - Effects of Intracerebroventricularly (ICV) Injected Ghrelin on Cardiac Inducible Nitric Oxide Synthase Activity/Expression in Obese Rats VL - 123 IS - 10 SP - 581 EP - 588 DO - 10.1055/s-0035-1559758 ER -
@article{ author = "Sudar, Emina and Jovanović, Aleksandra and Misirkić-Marjanović, Maja and Vučićević, Ljubica and Janjetović, Kristina D. and Isenović, Esma R.", year = "2015", abstract = "The aim of this study was to examine the effects of ghrelin on regulation of cardiac inducible nitric oxide synthase (iNOS) activity/expression in high fat (HF), obese rats. For this study, male Wistar rats fed with HF diet (30 % fat) for 4 weeks were injected every 24 h for 5 days intracerebroventriculary (ICV) with ghrelin (0.3 nmol/5 mu l) or with an equal volume of phosphate buffered saline (PBS). Control rats were ICV injected with an equal volume of PBS. Glucose, insulin and nitric oxide (NO) concentrations were measured in serum, while arginase activity and citrulline concentrations were measured in heart lysate. Protein iNOS and regulatory subunit of nuclear factor-kappa B (NF kappa B-p65), phosphorylation of enzymes protein kinase B (Akt) at Ser(473), and extracellular signal-regulated kinases 1/2 (ERK1/2) at Tyr(202)/Tyr(204) were determined in heart lysate by Western blot. For gene expression of iNOS qRT-PCR was used. Results show significantly (p LT 0.01) higher serum NO production in ghrelin treated HF rats compared with HF rats. Ghrelin significantly reduced citrulline concentration (p LT 0.05) and arginase activity (p LT 0.01) in HF rats. In ghrelin treated HF rats, gene and protein expression of iNOS and NF kappa B-p65 levels were significantly (p LT 0.05) increased compared with HF rats. Increased phosphorylation of Akt (p LT 0.01) and decreased (p LT 0.05) ERK1/2 phosphorylation were detected in HF ghrelin treated rats compared with HF rats hearts. Results from this study indicate that exogenous ghrelin induces expression and activity of cardiac iNOS via Akt phosphorylation followed by NF kappa B activation in HF rats.", publisher = "Georg Thieme Verlag KG", journal = "Experimental and Clinical Endocrinology and Diabetes", title = "Effects of Intracerebroventricularly (ICV) Injected Ghrelin on Cardiac Inducible Nitric Oxide Synthase Activity/Expression in Obese Rats", volume = "123", number = "10", pages = "581-588", doi = "10.1055/s-0035-1559758" }
Sudar, E., Jovanović, A., Misirkić-Marjanović, M., Vučićević, L., Janjetović, K. D.,& Isenović, E. R.. (2015). Effects of Intracerebroventricularly (ICV) Injected Ghrelin on Cardiac Inducible Nitric Oxide Synthase Activity/Expression in Obese Rats. in Experimental and Clinical Endocrinology and Diabetes Georg Thieme Verlag KG., 123(10), 581-588. https://doi.org/10.1055/s-0035-1559758
Sudar E, Jovanović A, Misirkić-Marjanović M, Vučićević L, Janjetović KD, Isenović ER. Effects of Intracerebroventricularly (ICV) Injected Ghrelin on Cardiac Inducible Nitric Oxide Synthase Activity/Expression in Obese Rats. in Experimental and Clinical Endocrinology and Diabetes. 2015;123(10):581-588. doi:10.1055/s-0035-1559758 .
Sudar, Emina, Jovanović, Aleksandra, Misirkić-Marjanović, Maja, Vučićević, Ljubica, Janjetović, Kristina D., Isenović, Esma R., "Effects of Intracerebroventricularly (ICV) Injected Ghrelin on Cardiac Inducible Nitric Oxide Synthase Activity/Expression in Obese Rats" in Experimental and Clinical Endocrinology and Diabetes, 123, no. 10 (2015):581-588, https://doi.org/10.1055/s-0035-1559758 . .