Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity
Само за регистроване кориснике
2015
Аутори
Todorović, AnaPejić, Snežana
Stojiljković, Vesna
Gavrilović, Ljubica
Popović, Nataša M.
Pavlović, Ivan
Saičić, Zorica S.
Pajović, Snežana B.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Purpose Previously, we examined manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (CuZnSOD), and catalase (CAT) activities in rat brain irradiated with 2 or 3 Gy of gamma-rays. The results indicated that lower MnSOD activity and inducibility found in hippocampus might explain higher radiosensitivity of this brain region. Thus, in this study, we wanted to determine changes of MnSOD, CuZnSOD, and CAT activities after dose of 5 Gy and to find out if differences in MnSOD activity are caused by changes in its expression. Heads of 4-day-old female rats were irradiated with gamma-rays, using Co-60. Animals were sacrificed 1/24 h after exposure. Hippocampus and cortex tissues were prepared for enzyme activity measurements and Western blot analysis. One hour after exposure, gamma-rays significantly decreased MnSOD activity in both examined brain regions. Twenty-four hours later, MnSOD recovery showed dose and regional dependence. It was weaker at higher doses and in hippo...campal region. MnSOD expression changed in the similar manner as MnSOD activity only at lower doses of gamma-rays. In both examined brain regions, gamma radiation significantly decreased CuZnSOD activity and did not change activity of CAT. Our results confirmed that MnSOD plays an important role in different regional radiosensitivity but also showed that depending on dose, radiation affects MnSOD level by utterly different mechanisms. Postradiation changes of CuZnSOD and CAT are not regionally specific and therefore, cannot account for the different radiosensitivity of the hippocampus and cortex.
Кључне речи:
SOD / CAT / Radiosensitivity / Brain / Hippocampus / CortexИзвор:
Childs Nervous System, 2015, 31, 12, 2249-2256Издавач:
- Springer Nature
Финансирање / пројекти:
- Ћелијске и молекулске основе малигних и кардиоваскуларних обољења-клиничке импликације (RS-41027)
- Акутни коронарни синдром: истраживање вулнерабилности (плака, крви и миокарда), оптимално лечење и одређивање прогностичких фактора (RS-41022)
DOI: 10.1007/s00381-015-2807-2
ISSN: 0256-7040; 1433-0350
PubMed: 26143278
WoS: 000365079000009
Scopus: 2-s2.0-84947031738
Колекције
Институција/група
VinčaTY - JOUR AU - Todorović, Ana AU - Pejić, Snežana AU - Stojiljković, Vesna AU - Gavrilović, Ljubica AU - Popović, Nataša M. AU - Pavlović, Ivan AU - Saičić, Zorica S. AU - Pajović, Snežana B. PY - 2015 UR - https://vinar.vin.bg.ac.rs/handle/123456789/815 AB - Purpose Previously, we examined manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (CuZnSOD), and catalase (CAT) activities in rat brain irradiated with 2 or 3 Gy of gamma-rays. The results indicated that lower MnSOD activity and inducibility found in hippocampus might explain higher radiosensitivity of this brain region. Thus, in this study, we wanted to determine changes of MnSOD, CuZnSOD, and CAT activities after dose of 5 Gy and to find out if differences in MnSOD activity are caused by changes in its expression. Heads of 4-day-old female rats were irradiated with gamma-rays, using Co-60. Animals were sacrificed 1/24 h after exposure. Hippocampus and cortex tissues were prepared for enzyme activity measurements and Western blot analysis. One hour after exposure, gamma-rays significantly decreased MnSOD activity in both examined brain regions. Twenty-four hours later, MnSOD recovery showed dose and regional dependence. It was weaker at higher doses and in hippocampal region. MnSOD expression changed in the similar manner as MnSOD activity only at lower doses of gamma-rays. In both examined brain regions, gamma radiation significantly decreased CuZnSOD activity and did not change activity of CAT. Our results confirmed that MnSOD plays an important role in different regional radiosensitivity but also showed that depending on dose, radiation affects MnSOD level by utterly different mechanisms. Postradiation changes of CuZnSOD and CAT are not regionally specific and therefore, cannot account for the different radiosensitivity of the hippocampus and cortex. PB - Springer Nature T2 - Childs Nervous System T1 - Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity VL - 31 IS - 12 SP - 2249 EP - 2256 DO - 10.1007/s00381-015-2807-2 ER -
@article{ author = "Todorović, Ana and Pejić, Snežana and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Pavlović, Ivan and Saičić, Zorica S. and Pajović, Snežana B.", year = "2015", abstract = "Purpose Previously, we examined manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (CuZnSOD), and catalase (CAT) activities in rat brain irradiated with 2 or 3 Gy of gamma-rays. The results indicated that lower MnSOD activity and inducibility found in hippocampus might explain higher radiosensitivity of this brain region. Thus, in this study, we wanted to determine changes of MnSOD, CuZnSOD, and CAT activities after dose of 5 Gy and to find out if differences in MnSOD activity are caused by changes in its expression. Heads of 4-day-old female rats were irradiated with gamma-rays, using Co-60. Animals were sacrificed 1/24 h after exposure. Hippocampus and cortex tissues were prepared for enzyme activity measurements and Western blot analysis. One hour after exposure, gamma-rays significantly decreased MnSOD activity in both examined brain regions. Twenty-four hours later, MnSOD recovery showed dose and regional dependence. It was weaker at higher doses and in hippocampal region. MnSOD expression changed in the similar manner as MnSOD activity only at lower doses of gamma-rays. In both examined brain regions, gamma radiation significantly decreased CuZnSOD activity and did not change activity of CAT. Our results confirmed that MnSOD plays an important role in different regional radiosensitivity but also showed that depending on dose, radiation affects MnSOD level by utterly different mechanisms. Postradiation changes of CuZnSOD and CAT are not regionally specific and therefore, cannot account for the different radiosensitivity of the hippocampus and cortex.", publisher = "Springer Nature", journal = "Childs Nervous System", title = "Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity", volume = "31", number = "12", pages = "2249-2256", doi = "10.1007/s00381-015-2807-2" }
Todorović, A., Pejić, S., Stojiljković, V., Gavrilović, L., Popović, N. M., Pavlović, I., Saičić, Z. S.,& Pajović, S. B.. (2015). Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity. in Childs Nervous System Springer Nature., 31(12), 2249-2256. https://doi.org/10.1007/s00381-015-2807-2
Todorović A, Pejić S, Stojiljković V, Gavrilović L, Popović NM, Pavlović I, Saičić ZS, Pajović SB. Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity. in Childs Nervous System. 2015;31(12):2249-2256. doi:10.1007/s00381-015-2807-2 .
Todorović, Ana, Pejić, Snežana, Stojiljković, Vesna, Gavrilović, Ljubica, Popović, Nataša M., Pavlović, Ivan, Saičić, Zorica S., Pajović, Snežana B., "Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity" in Childs Nervous System, 31, no. 12 (2015):2249-2256, https://doi.org/10.1007/s00381-015-2807-2 . .