Adsorption of Candida rugosa lipase onto alumina: effect of surface charge
2015
Аутори
Izrael-Živković, Lidija T.Živković, Ljiljana
Jokić, Bojan M.
Savić, Andrija B.
Karadzic, Ivanka M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The impact of the surface charge of alumina supports on the adsorption of Candida rugosa lipase was investigated in terms of the zeta potentials of the adsorption partners. The lipase adhered onto alumina with similar efficiency under both repulsive and attractive electrostatic conditions, shifting the zeta potential of the support towards that of the enzyme. The behavior was explained by a heterogeneous distribution of the surface charge of the lipase molecule. Special emphasis in this study was placed on the effect of immobilization on the enzyme kinetics and principal reasons for enzyme immobilization: improvement in stability and potential for reuse. The enzyme affinity was not altered by its adsorption onto alumina, while the Vmax value of the lipase decreased. The thermostability of the adsorbed lipase was improved. A significant potential for reuse was found.
Кључне речи:
alumina / lipase / Adsorption / immobilization / Candida rugosaИзвор:
Journal of the Serbian Chemical Society, 2015, 80, 9, 1113-1125Финансирање / пројекти:
- Синтеза, процесирање и карактеризација наноструктурних материјала за примену у области енергије, механичког инжењерства, заштите животне стредине и биомедицине (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45012)
- Симултана биоремедијација и соилификација деградираних простора, за очување природних ресурса биолошки активних супстанци и развој и производњу биоматеријала и дијететских производа (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43004)
DOI: 10.2298/JSC150222035I
ISSN: 0352-5139
WoS: 000364205600002
Scopus: 2-s2.0-84947609900
Колекције
Институција/група
VinčaTY - JOUR AU - Izrael-Živković, Lidija T. AU - Živković, Ljiljana AU - Jokić, Bojan M. AU - Savić, Andrija B. AU - Karadzic, Ivanka M. PY - 2015 UR - https://vinar.vin.bg.ac.rs/handle/123456789/796 AB - The impact of the surface charge of alumina supports on the adsorption of Candida rugosa lipase was investigated in terms of the zeta potentials of the adsorption partners. The lipase adhered onto alumina with similar efficiency under both repulsive and attractive electrostatic conditions, shifting the zeta potential of the support towards that of the enzyme. The behavior was explained by a heterogeneous distribution of the surface charge of the lipase molecule. Special emphasis in this study was placed on the effect of immobilization on the enzyme kinetics and principal reasons for enzyme immobilization: improvement in stability and potential for reuse. The enzyme affinity was not altered by its adsorption onto alumina, while the Vmax value of the lipase decreased. The thermostability of the adsorbed lipase was improved. A significant potential for reuse was found. T2 - Journal of the Serbian Chemical Society T1 - Adsorption of Candida rugosa lipase onto alumina: effect of surface charge VL - 80 IS - 9 SP - 1113 EP - 1125 DO - 10.2298/JSC150222035I ER -
@article{ author = "Izrael-Živković, Lidija T. and Živković, Ljiljana and Jokić, Bojan M. and Savić, Andrija B. and Karadzic, Ivanka M.", year = "2015", abstract = "The impact of the surface charge of alumina supports on the adsorption of Candida rugosa lipase was investigated in terms of the zeta potentials of the adsorption partners. The lipase adhered onto alumina with similar efficiency under both repulsive and attractive electrostatic conditions, shifting the zeta potential of the support towards that of the enzyme. The behavior was explained by a heterogeneous distribution of the surface charge of the lipase molecule. Special emphasis in this study was placed on the effect of immobilization on the enzyme kinetics and principal reasons for enzyme immobilization: improvement in stability and potential for reuse. The enzyme affinity was not altered by its adsorption onto alumina, while the Vmax value of the lipase decreased. The thermostability of the adsorbed lipase was improved. A significant potential for reuse was found.", journal = "Journal of the Serbian Chemical Society", title = "Adsorption of Candida rugosa lipase onto alumina: effect of surface charge", volume = "80", number = "9", pages = "1113-1125", doi = "10.2298/JSC150222035I" }
Izrael-Živković, L. T., Živković, L., Jokić, B. M., Savić, A. B.,& Karadzic, I. M.. (2015). Adsorption of Candida rugosa lipase onto alumina: effect of surface charge. in Journal of the Serbian Chemical Society, 80(9), 1113-1125. https://doi.org/10.2298/JSC150222035I
Izrael-Živković LT, Živković L, Jokić BM, Savić AB, Karadzic IM. Adsorption of Candida rugosa lipase onto alumina: effect of surface charge. in Journal of the Serbian Chemical Society. 2015;80(9):1113-1125. doi:10.2298/JSC150222035I .
Izrael-Živković, Lidija T., Živković, Ljiljana, Jokić, Bojan M., Savić, Andrija B., Karadzic, Ivanka M., "Adsorption of Candida rugosa lipase onto alumina: effect of surface charge" in Journal of the Serbian Chemical Society, 80, no. 9 (2015):1113-1125, https://doi.org/10.2298/JSC150222035I . .