Spectrophotometric determination of desoximetasone in ointment using 1,4-dihydrazinophthalazine
Апстракт
The proposed method is based on coloured hydrazone formation with 1,4-dihydrazinophthalazine as a reagent. Heating at 85 degrees C for 2 h was found necessary to ensure optimal hydrazone formation in the presence of hydrochloric acid. The yellow hydrazone product has an absorption maximum at 380 nm. A linear relationship between absorbance and concentration was established in the concentration range 3.19 x 10(-6)-3.19 x 10(-5) mol l(-1) (the regression equation was y = 0.013 167 3 + 0.019 025 9x; correlation coefficient r = 0.9991; n = 6). The detection limit was 1.2 mu g ml(-1) (molar absorptivity found was 1.97 x 10(4) l mol(-1) cm(-1)). The reliability of the proposed method was checked at three different concentrations; the relative standard deviation (RSD) varied from 1.03 to 2.01%. The described method applied to the determination of desoximetasone in ointment gave precise and reproducible results; the recovery was 98.55% with RSD = 2.40% (n = 10).
Кључне речи:
desoximetasone / 1,4-dihydrazinophthalazine / ointment / spectrophotometryИзвор:
Journal of Pharmaceutical and Biomedical Analysis, 1996, 14, 8-10, 947-950Напомена:
- 5th International Symposium on Drug Analysis, Sep 12-15, 1995, Louvain, Belgium
DOI: 10.1016/0731-7085(95)01681-3
ISSN: 0731-7085
PubMed: 8817999
WoS: A1996UY34400011
Scopus: 2-s2.0-0030176421
Колекције
Институција/група
VinčaTY - JOUR AU - Vladimirov, Sote AU - Čudina, Olivera AU - Agbaba, Danica AU - Jovanovic, M AU - Živanov-Stakić, Dobrila PY - 1996 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7217 AB - The proposed method is based on coloured hydrazone formation with 1,4-dihydrazinophthalazine as a reagent. Heating at 85 degrees C for 2 h was found necessary to ensure optimal hydrazone formation in the presence of hydrochloric acid. The yellow hydrazone product has an absorption maximum at 380 nm. A linear relationship between absorbance and concentration was established in the concentration range 3.19 x 10(-6)-3.19 x 10(-5) mol l(-1) (the regression equation was y = 0.013 167 3 + 0.019 025 9x; correlation coefficient r = 0.9991; n = 6). The detection limit was 1.2 mu g ml(-1) (molar absorptivity found was 1.97 x 10(4) l mol(-1) cm(-1)). The reliability of the proposed method was checked at three different concentrations; the relative standard deviation (RSD) varied from 1.03 to 2.01%. The described method applied to the determination of desoximetasone in ointment gave precise and reproducible results; the recovery was 98.55% with RSD = 2.40% (n = 10). T2 - Journal of Pharmaceutical and Biomedical Analysis T1 - Spectrophotometric determination of desoximetasone in ointment using 1,4-dihydrazinophthalazine VL - 14 IS - 8-10 SP - 947 EP - 950 DO - 10.1016/0731-7085(95)01681-3 ER -
@article{ author = "Vladimirov, Sote and Čudina, Olivera and Agbaba, Danica and Jovanovic, M and Živanov-Stakić, Dobrila", year = "1996", abstract = "The proposed method is based on coloured hydrazone formation with 1,4-dihydrazinophthalazine as a reagent. Heating at 85 degrees C for 2 h was found necessary to ensure optimal hydrazone formation in the presence of hydrochloric acid. The yellow hydrazone product has an absorption maximum at 380 nm. A linear relationship between absorbance and concentration was established in the concentration range 3.19 x 10(-6)-3.19 x 10(-5) mol l(-1) (the regression equation was y = 0.013 167 3 + 0.019 025 9x; correlation coefficient r = 0.9991; n = 6). The detection limit was 1.2 mu g ml(-1) (molar absorptivity found was 1.97 x 10(4) l mol(-1) cm(-1)). The reliability of the proposed method was checked at three different concentrations; the relative standard deviation (RSD) varied from 1.03 to 2.01%. The described method applied to the determination of desoximetasone in ointment gave precise and reproducible results; the recovery was 98.55% with RSD = 2.40% (n = 10).", journal = "Journal of Pharmaceutical and Biomedical Analysis", title = "Spectrophotometric determination of desoximetasone in ointment using 1,4-dihydrazinophthalazine", volume = "14", number = "8-10", pages = "947-950", doi = "10.1016/0731-7085(95)01681-3" }
Vladimirov, S., Čudina, O., Agbaba, D., Jovanovic, M.,& Živanov-Stakić, D.. (1996). Spectrophotometric determination of desoximetasone in ointment using 1,4-dihydrazinophthalazine. in Journal of Pharmaceutical and Biomedical Analysis, 14(8-10), 947-950. https://doi.org/10.1016/0731-7085(95)01681-3
Vladimirov S, Čudina O, Agbaba D, Jovanovic M, Živanov-Stakić D. Spectrophotometric determination of desoximetasone in ointment using 1,4-dihydrazinophthalazine. in Journal of Pharmaceutical and Biomedical Analysis. 1996;14(8-10):947-950. doi:10.1016/0731-7085(95)01681-3 .
Vladimirov, Sote, Čudina, Olivera, Agbaba, Danica, Jovanovic, M, Živanov-Stakić, Dobrila, "Spectrophotometric determination of desoximetasone in ointment using 1,4-dihydrazinophthalazine" in Journal of Pharmaceutical and Biomedical Analysis, 14, no. 8-10 (1996):947-950, https://doi.org/10.1016/0731-7085(95)01681-3 . .