Response of a human melanoma cell line to low and high ionizing radiation
Само за регистроване кориснике
2007
Аутори
Ristić-Fira, AleksandraTodorović, Danijela V.
Korićanac, Lela
Petrović, Ivan M.
Valastro, Lucia M.
Cirrone, Giuseppe Antonio Pablo
Raffaele, Luigi
Cuttone, Giacomo
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Effects of single irradiation with gamma rays and protons on human HTB140 melanoma cell growth were compared. Exponentially growing cells were irradiated close to the Bragg peak maximum of the unmodulated 62 MeV protons, as well as with Co-60 gamma rays. Applied doses ranged from 8 to 24 Gy. Viability of cells and proliferation capacity were assessed 7 days after irradiation. Induction of apoptosis and cell cycle phase redistribution were observed 6 and 48 h after irradiation. Significant inhibitory effects of both irradiation qualities were detected 7 days after irradiation. Important reduction of HTB140 cell viability was observed after irradiation with protons. Almost linear and highly significant (P LT 0.001) decrease of cell proliferation was observed 7 days after irradiation with gamma rays and protons, as compared to nonirradiated controls. Protons induced apoptosis, both 6 and 48 h after irradiation. With the increase of post-irradiation incubation time, number of apoptotic cel...ls decreased. Exposure of HTB140 cells to gamma rays did not provoke apoptotic cell death. Important number of cells in G1-S phase, detected by the cell cycle phase redistribution analyses, suggested high metabolic activity of irradiated melanoma cells within the first 48 h. Both irradiation qualities caused modest G2-M arrest 6 and 48 h after irradiation, thus supporting results that illustrated high radioresistance of HTB140 cells.
Кључне речи:
melanoma / gamma rays / protons / proliferation capacity / apoptosis / cell cycle phase redistributionИзвор:
Annals of the New York Academy of Sciences, 2007, 1095, 165-174Напомена:
- Cell Signaling World 2006 Conference, Jan 25-28, 2006, Luxembourg, Luxembourg
DOI: 10.1196/annals.1397.020
ISSN: 0077-8923
PubMed: 17404029
WoS: 000245947400019
Scopus: 2-s2.0-34247860709
Институција/група
VinčaTY - CONF AU - Ristić-Fira, Aleksandra AU - Todorović, Danijela V. AU - Korićanac, Lela AU - Petrović, Ivan M. AU - Valastro, Lucia M. AU - Cirrone, Giuseppe Antonio Pablo AU - Raffaele, Luigi AU - Cuttone, Giacomo PY - 2007 UR - https://vinar.vin.bg.ac.rs/handle/123456789/6648 AB - Effects of single irradiation with gamma rays and protons on human HTB140 melanoma cell growth were compared. Exponentially growing cells were irradiated close to the Bragg peak maximum of the unmodulated 62 MeV protons, as well as with Co-60 gamma rays. Applied doses ranged from 8 to 24 Gy. Viability of cells and proliferation capacity were assessed 7 days after irradiation. Induction of apoptosis and cell cycle phase redistribution were observed 6 and 48 h after irradiation. Significant inhibitory effects of both irradiation qualities were detected 7 days after irradiation. Important reduction of HTB140 cell viability was observed after irradiation with protons. Almost linear and highly significant (P LT 0.001) decrease of cell proliferation was observed 7 days after irradiation with gamma rays and protons, as compared to nonirradiated controls. Protons induced apoptosis, both 6 and 48 h after irradiation. With the increase of post-irradiation incubation time, number of apoptotic cells decreased. Exposure of HTB140 cells to gamma rays did not provoke apoptotic cell death. Important number of cells in G1-S phase, detected by the cell cycle phase redistribution analyses, suggested high metabolic activity of irradiated melanoma cells within the first 48 h. Both irradiation qualities caused modest G2-M arrest 6 and 48 h after irradiation, thus supporting results that illustrated high radioresistance of HTB140 cells. C3 - Annals of the New York Academy of Sciences T1 - Response of a human melanoma cell line to low and high ionizing radiation VL - 1095 SP - 165 EP - 174 DO - 10.1196/annals.1397.020 ER -
@conference{ author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Korićanac, Lela and Petrović, Ivan M. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Raffaele, Luigi and Cuttone, Giacomo", year = "2007", abstract = "Effects of single irradiation with gamma rays and protons on human HTB140 melanoma cell growth were compared. Exponentially growing cells were irradiated close to the Bragg peak maximum of the unmodulated 62 MeV protons, as well as with Co-60 gamma rays. Applied doses ranged from 8 to 24 Gy. Viability of cells and proliferation capacity were assessed 7 days after irradiation. Induction of apoptosis and cell cycle phase redistribution were observed 6 and 48 h after irradiation. Significant inhibitory effects of both irradiation qualities were detected 7 days after irradiation. Important reduction of HTB140 cell viability was observed after irradiation with protons. Almost linear and highly significant (P LT 0.001) decrease of cell proliferation was observed 7 days after irradiation with gamma rays and protons, as compared to nonirradiated controls. Protons induced apoptosis, both 6 and 48 h after irradiation. With the increase of post-irradiation incubation time, number of apoptotic cells decreased. Exposure of HTB140 cells to gamma rays did not provoke apoptotic cell death. Important number of cells in G1-S phase, detected by the cell cycle phase redistribution analyses, suggested high metabolic activity of irradiated melanoma cells within the first 48 h. Both irradiation qualities caused modest G2-M arrest 6 and 48 h after irradiation, thus supporting results that illustrated high radioresistance of HTB140 cells.", journal = "Annals of the New York Academy of Sciences", title = "Response of a human melanoma cell line to low and high ionizing radiation", volume = "1095", pages = "165-174", doi = "10.1196/annals.1397.020" }
Ristić-Fira, A., Todorović, D. V., Korićanac, L., Petrović, I. M., Valastro, L. M., Cirrone, G. A. P., Raffaele, L.,& Cuttone, G.. (2007). Response of a human melanoma cell line to low and high ionizing radiation. in Annals of the New York Academy of Sciences, 1095, 165-174. https://doi.org/10.1196/annals.1397.020
Ristić-Fira A, Todorović DV, Korićanac L, Petrović IM, Valastro LM, Cirrone GAP, Raffaele L, Cuttone G. Response of a human melanoma cell line to low and high ionizing radiation. in Annals of the New York Academy of Sciences. 2007;1095:165-174. doi:10.1196/annals.1397.020 .
Ristić-Fira, Aleksandra, Todorović, Danijela V., Korićanac, Lela, Petrović, Ivan M., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Raffaele, Luigi, Cuttone, Giacomo, "Response of a human melanoma cell line to low and high ionizing radiation" in Annals of the New York Academy of Sciences, 1095 (2007):165-174, https://doi.org/10.1196/annals.1397.020 . .