Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters
Само за регистроване кориснике
2014
Аутори
Živković, MajaStanković, Aleksandra
Đurić, Tamara
Končar, Igor
Kolaković, Ana
Đurđević, Vladimir
Davidović, Lazar
Alavantić, Dragan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Glutathione S-transferases (GSTs) carry out a wide range of functions in cells, such as detoxification of endogenous compounds, removal of reactive oxygen species, and even catalysis of reactions in metabolic pathways beyond detoxification. Based on previous research, GSTM1 and GSTT1 might modify the risk of atherosclerosis. The aim of our study was to analyze the possible association of GSTM1 and GSTT1 gene polymorphisms with the occurrence of carotid plaque (CP); and biochemical parameters of oxidative stress, lipid profile and inflammation, in 346 consecutive patients with advanced atherosclerosis that underwent endarterectomy. A multiplex polymerase chain reaction (PCR) method was used to detect the deletions in GSTM1 and GSTT1 genes in the genomic DNA in 346 patients and 330 controls. The adjusted OR for CP presence (adjusted for age, gender, smoking, hypertension, BMI, HDLC, TG) was 0.24, 95 %CI 0.08-0.7, p LT 0.01 for GSTT1 null and 1.13, 95 %CI 0.62-2.07, p = 0.7 for GSTM1 null... genotype. We found significantly lower plasma lipoprotein (a) (Lp(a)) levels in GSTT1 null compared to wild-type genotype carriers in patient group (20.68 +/- A 26.02 mg/dl vs. 40.66 +/- A 42.89 mg/dl, mean +/- A SD, p = 0.04). The serum interleukin-6 (IL-6) values were significantly influenced by both GST polymorphisms in patients with CP. Our results, showing the significant reduction of GSTT1 deletions in patients with CP, suggest involvement of GSTs in carotid atherosclerosis. This study shows additional view of the possible role of GSTs in advanced chronic inflammatory disease of vascular system, but the confirmation in a larger studies in different populations are needed.
Кључне речи:
GSTT1 / GSTM1 / Polymorphisms / Carotid plaque / Lipids / InflammationИзвор:
Molecular Biology Reports, 2014, 41, 2, 1157-1164Финансирање / пројекти:
- Генетска основа хуманих васкуларних и инфламаторних болести (RS-MESTD-Basic Research (BR or ON)-175085)
- Интегрална студија идентификације регионалних генетских фактора ризика и фактора ризика животне средине за масовне незаразне болести хумане популације у Србији - INGEMA_S (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41028)
DOI: 10.1007/s11033-013-2962-z
ISSN: 0301-4851; 1573-4978
PubMed: 24407598
WoS: 000330860900065
Scopus: 2-s2.0-84899459637
Колекције
Институција/група
VinčaTY - JOUR AU - Živković, Maja AU - Stanković, Aleksandra AU - Đurić, Tamara AU - Končar, Igor AU - Kolaković, Ana AU - Đurđević, Vladimir AU - Davidović, Lazar AU - Alavantić, Dragan PY - 2014 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5869 AB - Glutathione S-transferases (GSTs) carry out a wide range of functions in cells, such as detoxification of endogenous compounds, removal of reactive oxygen species, and even catalysis of reactions in metabolic pathways beyond detoxification. Based on previous research, GSTM1 and GSTT1 might modify the risk of atherosclerosis. The aim of our study was to analyze the possible association of GSTM1 and GSTT1 gene polymorphisms with the occurrence of carotid plaque (CP); and biochemical parameters of oxidative stress, lipid profile and inflammation, in 346 consecutive patients with advanced atherosclerosis that underwent endarterectomy. A multiplex polymerase chain reaction (PCR) method was used to detect the deletions in GSTM1 and GSTT1 genes in the genomic DNA in 346 patients and 330 controls. The adjusted OR for CP presence (adjusted for age, gender, smoking, hypertension, BMI, HDLC, TG) was 0.24, 95 %CI 0.08-0.7, p LT 0.01 for GSTT1 null and 1.13, 95 %CI 0.62-2.07, p = 0.7 for GSTM1 null genotype. We found significantly lower plasma lipoprotein (a) (Lp(a)) levels in GSTT1 null compared to wild-type genotype carriers in patient group (20.68 +/- A 26.02 mg/dl vs. 40.66 +/- A 42.89 mg/dl, mean +/- A SD, p = 0.04). The serum interleukin-6 (IL-6) values were significantly influenced by both GST polymorphisms in patients with CP. Our results, showing the significant reduction of GSTT1 deletions in patients with CP, suggest involvement of GSTs in carotid atherosclerosis. This study shows additional view of the possible role of GSTs in advanced chronic inflammatory disease of vascular system, but the confirmation in a larger studies in different populations are needed. T2 - Molecular Biology Reports T1 - Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters VL - 41 IS - 2 SP - 1157 EP - 1164 DO - 10.1007/s11033-013-2962-z ER -
@article{ author = "Živković, Maja and Stanković, Aleksandra and Đurić, Tamara and Končar, Igor and Kolaković, Ana and Đurđević, Vladimir and Davidović, Lazar and Alavantić, Dragan", year = "2014", abstract = "Glutathione S-transferases (GSTs) carry out a wide range of functions in cells, such as detoxification of endogenous compounds, removal of reactive oxygen species, and even catalysis of reactions in metabolic pathways beyond detoxification. Based on previous research, GSTM1 and GSTT1 might modify the risk of atherosclerosis. The aim of our study was to analyze the possible association of GSTM1 and GSTT1 gene polymorphisms with the occurrence of carotid plaque (CP); and biochemical parameters of oxidative stress, lipid profile and inflammation, in 346 consecutive patients with advanced atherosclerosis that underwent endarterectomy. A multiplex polymerase chain reaction (PCR) method was used to detect the deletions in GSTM1 and GSTT1 genes in the genomic DNA in 346 patients and 330 controls. The adjusted OR for CP presence (adjusted for age, gender, smoking, hypertension, BMI, HDLC, TG) was 0.24, 95 %CI 0.08-0.7, p LT 0.01 for GSTT1 null and 1.13, 95 %CI 0.62-2.07, p = 0.7 for GSTM1 null genotype. We found significantly lower plasma lipoprotein (a) (Lp(a)) levels in GSTT1 null compared to wild-type genotype carriers in patient group (20.68 +/- A 26.02 mg/dl vs. 40.66 +/- A 42.89 mg/dl, mean +/- A SD, p = 0.04). The serum interleukin-6 (IL-6) values were significantly influenced by both GST polymorphisms in patients with CP. Our results, showing the significant reduction of GSTT1 deletions in patients with CP, suggest involvement of GSTs in carotid atherosclerosis. This study shows additional view of the possible role of GSTs in advanced chronic inflammatory disease of vascular system, but the confirmation in a larger studies in different populations are needed.", journal = "Molecular Biology Reports", title = "Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters", volume = "41", number = "2", pages = "1157-1164", doi = "10.1007/s11033-013-2962-z" }
Živković, M., Stanković, A., Đurić, T., Končar, I., Kolaković, A., Đurđević, V., Davidović, L.,& Alavantić, D.. (2014). Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters. in Molecular Biology Reports, 41(2), 1157-1164. https://doi.org/10.1007/s11033-013-2962-z
Živković M, Stanković A, Đurić T, Končar I, Kolaković A, Đurđević V, Davidović L, Alavantić D. Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters. in Molecular Biology Reports. 2014;41(2):1157-1164. doi:10.1007/s11033-013-2962-z .
Živković, Maja, Stanković, Aleksandra, Đurić, Tamara, Končar, Igor, Kolaković, Ana, Đurđević, Vladimir, Davidović, Lazar, Alavantić, Dragan, "Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters" in Molecular Biology Reports, 41, no. 2 (2014):1157-1164, https://doi.org/10.1007/s11033-013-2962-z . .