The Effect of Different Acute Concentrations of Cadmium Chloride on the Frequency of Micronuclei in Ao Rats
Аутори
Popovic Bubujuk, SlavicaBojat, Nenad C.
Đelić, Ninoslav
Dronjak, Slađana
Kostadinovic, Ljiljana
Coghill Galonja, Tamara
Anđelković, Marko
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cadmium (Cd) is highly toxic heavy metal which may cause severe biological effects in vivo and in vitro. In this study, an evaluation of the acute Cd ability to trigger micronuclei (MNi) formation was carried out on 3-month-old male and female Albino Oxford (AO) rats using micronucleus (MN) test. Experimental animals were treated intraperitoneally with three different concentrations of cadmium chloride (CdCl2): 0.5, 1, and 2 mg CdCl2 per kg of body weight. Control animals received equal volume of sterile phosphate buffered saline. The results showed that 2 mg CdCl2 per kg b.w. concentration caused a highly statistically significant (P LT 0.001) increase in MNi formation in the bone marrow polychromatic erythrocytes (PCEs), exerting a clear-cut concentration-dependent effect. Lower concentrations of CdCl2 used (0.5 and 1 mg/kg b.w.) also caused MNi formation, but with lower statistical significance. Sex differences in MNi production in bone marrow PCEs after acute exposure to different ...experimental concentrations of CdCl2 were not observed in our study. Our results indicate the ability of CdCl2 to exerts genotoxic effects in bone marrow of AO rats, and complement previous data on the genotoxicity of this important environmental contaminant, burdening the body from different sources-major being industrial exposure, food and cigarette smoking.
Кључне речи:
Albino Oxford rats / cadmium / genotoxicity / micronucleus testИзвор:
Genetika, 2013, 45, 3, 727-736Финансирање / пројекти:
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.2298/GENSR1303727B
ISSN: 0534-0012; 1820-6069
WoS: 000330186600010
Scopus: 2-s2.0-84894539041
Колекције
Институција/група
VinčaTY - JOUR AU - Popovic Bubujuk, Slavica AU - Bojat, Nenad C. AU - Đelić, Ninoslav AU - Dronjak, Slađana AU - Kostadinovic, Ljiljana AU - Coghill Galonja, Tamara AU - Anđelković, Marko PY - 2013 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5852 AB - Cadmium (Cd) is highly toxic heavy metal which may cause severe biological effects in vivo and in vitro. In this study, an evaluation of the acute Cd ability to trigger micronuclei (MNi) formation was carried out on 3-month-old male and female Albino Oxford (AO) rats using micronucleus (MN) test. Experimental animals were treated intraperitoneally with three different concentrations of cadmium chloride (CdCl2): 0.5, 1, and 2 mg CdCl2 per kg of body weight. Control animals received equal volume of sterile phosphate buffered saline. The results showed that 2 mg CdCl2 per kg b.w. concentration caused a highly statistically significant (P LT 0.001) increase in MNi formation in the bone marrow polychromatic erythrocytes (PCEs), exerting a clear-cut concentration-dependent effect. Lower concentrations of CdCl2 used (0.5 and 1 mg/kg b.w.) also caused MNi formation, but with lower statistical significance. Sex differences in MNi production in bone marrow PCEs after acute exposure to different experimental concentrations of CdCl2 were not observed in our study. Our results indicate the ability of CdCl2 to exerts genotoxic effects in bone marrow of AO rats, and complement previous data on the genotoxicity of this important environmental contaminant, burdening the body from different sources-major being industrial exposure, food and cigarette smoking. T2 - Genetika T1 - The Effect of Different Acute Concentrations of Cadmium Chloride on the Frequency of Micronuclei in Ao Rats VL - 45 IS - 3 SP - 727 EP - 736 DO - 10.2298/GENSR1303727B ER -
@article{ author = "Popovic Bubujuk, Slavica and Bojat, Nenad C. and Đelić, Ninoslav and Dronjak, Slađana and Kostadinovic, Ljiljana and Coghill Galonja, Tamara and Anđelković, Marko", year = "2013", abstract = "Cadmium (Cd) is highly toxic heavy metal which may cause severe biological effects in vivo and in vitro. In this study, an evaluation of the acute Cd ability to trigger micronuclei (MNi) formation was carried out on 3-month-old male and female Albino Oxford (AO) rats using micronucleus (MN) test. Experimental animals were treated intraperitoneally with three different concentrations of cadmium chloride (CdCl2): 0.5, 1, and 2 mg CdCl2 per kg of body weight. Control animals received equal volume of sterile phosphate buffered saline. The results showed that 2 mg CdCl2 per kg b.w. concentration caused a highly statistically significant (P LT 0.001) increase in MNi formation in the bone marrow polychromatic erythrocytes (PCEs), exerting a clear-cut concentration-dependent effect. Lower concentrations of CdCl2 used (0.5 and 1 mg/kg b.w.) also caused MNi formation, but with lower statistical significance. Sex differences in MNi production in bone marrow PCEs after acute exposure to different experimental concentrations of CdCl2 were not observed in our study. Our results indicate the ability of CdCl2 to exerts genotoxic effects in bone marrow of AO rats, and complement previous data on the genotoxicity of this important environmental contaminant, burdening the body from different sources-major being industrial exposure, food and cigarette smoking.", journal = "Genetika", title = "The Effect of Different Acute Concentrations of Cadmium Chloride on the Frequency of Micronuclei in Ao Rats", volume = "45", number = "3", pages = "727-736", doi = "10.2298/GENSR1303727B" }
Popovic Bubujuk, S., Bojat, N. C., Đelić, N., Dronjak, S., Kostadinovic, L., Coghill Galonja, T.,& Anđelković, M.. (2013). The Effect of Different Acute Concentrations of Cadmium Chloride on the Frequency of Micronuclei in Ao Rats. in Genetika, 45(3), 727-736. https://doi.org/10.2298/GENSR1303727B
Popovic Bubujuk S, Bojat NC, Đelić N, Dronjak S, Kostadinovic L, Coghill Galonja T, Anđelković M. The Effect of Different Acute Concentrations of Cadmium Chloride on the Frequency of Micronuclei in Ao Rats. in Genetika. 2013;45(3):727-736. doi:10.2298/GENSR1303727B .
Popovic Bubujuk, Slavica, Bojat, Nenad C., Đelić, Ninoslav, Dronjak, Slađana, Kostadinovic, Ljiljana, Coghill Galonja, Tamara, Anđelković, Marko, "The Effect of Different Acute Concentrations of Cadmium Chloride on the Frequency of Micronuclei in Ao Rats" in Genetika, 45, no. 3 (2013):727-736, https://doi.org/10.2298/GENSR1303727B . .