Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex
Нема приказа
Аутори
Đikić, DragoslavaBudec, Mirela
Vranješ-Đurić, Sanja
Todorović, Vera
Drndarević, Neda C.
Vignjevic, Sanja
Mitrovic, Olivera
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
Background: This study was performed to investigate expression and distribution of glucocorticoid receptor (GR) in the rat adrenal cortex, acute effect of ethanol on its expression and possible role of endogenous nitric oxide (NO) in this phenomenon. Methods: Adult female Wistar rats showing diestrus day 1 were treated with: a) ethanol (2 or 4 g/kg body weight (b.w.), ip), b) N-omega-nitro-L-arginine methyl ester (L-NAME), well-known competitive inhibitor of all isoforms of NO synthase (NOS), (30 mg/kg b.w., sc) followed by ethanol (4 g/kg, ip) 3 h later and c) L-NAME (30 mg/kg b.w., sc) followed by saline (ip) 3 h later. Untreated rats were used as controls. Adrenocortical expression of GR was estimated by immunohistochemistry. Results: Strong nuclear GR staining was observed throughout the cortex of control rats. Acute ethanol treatment significantly decreased the expression of GR in the zona fasciculata and zona reticularis. Blockade of NO formation had no influence on this effect o...f ethanol, whereas L-NAME itself induced significant decline in GR immunoreactivity. Conclusions: Obtained findings are the first to demonstrate localization and distribution of the GR throughout the rat adrenal cortex and to suggest that ethanol as well as endogenous NO may modulate adrenocortical expression of this steroid receptor.
Кључне речи:
ethanol / L-NAME / glucocorticoid receptor / adrenal cortexИзвор:
Pharmacological Reports, 2012, 64, 4, 896-901Финансирање / пројекти:
- Испитивање патогенезе хематолошких малигнитета (RS-MESTD-Basic Research (BR or ON)-175053)
DOI: 10.1016/S1734-1140(12)70884-8
ISSN: 1734-1140
PubMed: 23087141
WoS: 000310420500012
Scopus: 2-s2.0-84867678044
Колекције
Институција/група
VinčaTY - JOUR AU - Đikić, Dragoslava AU - Budec, Mirela AU - Vranješ-Đurić, Sanja AU - Todorović, Vera AU - Drndarević, Neda C. AU - Vignjevic, Sanja AU - Mitrovic, Olivera PY - 2012 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5130 AB - Background: This study was performed to investigate expression and distribution of glucocorticoid receptor (GR) in the rat adrenal cortex, acute effect of ethanol on its expression and possible role of endogenous nitric oxide (NO) in this phenomenon. Methods: Adult female Wistar rats showing diestrus day 1 were treated with: a) ethanol (2 or 4 g/kg body weight (b.w.), ip), b) N-omega-nitro-L-arginine methyl ester (L-NAME), well-known competitive inhibitor of all isoforms of NO synthase (NOS), (30 mg/kg b.w., sc) followed by ethanol (4 g/kg, ip) 3 h later and c) L-NAME (30 mg/kg b.w., sc) followed by saline (ip) 3 h later. Untreated rats were used as controls. Adrenocortical expression of GR was estimated by immunohistochemistry. Results: Strong nuclear GR staining was observed throughout the cortex of control rats. Acute ethanol treatment significantly decreased the expression of GR in the zona fasciculata and zona reticularis. Blockade of NO formation had no influence on this effect of ethanol, whereas L-NAME itself induced significant decline in GR immunoreactivity. Conclusions: Obtained findings are the first to demonstrate localization and distribution of the GR throughout the rat adrenal cortex and to suggest that ethanol as well as endogenous NO may modulate adrenocortical expression of this steroid receptor. T2 - Pharmacological Reports T1 - Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex VL - 64 IS - 4 SP - 896 EP - 901 DO - 10.1016/S1734-1140(12)70884-8 ER -
@article{ author = "Đikić, Dragoslava and Budec, Mirela and Vranješ-Đurić, Sanja and Todorović, Vera and Drndarević, Neda C. and Vignjevic, Sanja and Mitrovic, Olivera", year = "2012", abstract = "Background: This study was performed to investigate expression and distribution of glucocorticoid receptor (GR) in the rat adrenal cortex, acute effect of ethanol on its expression and possible role of endogenous nitric oxide (NO) in this phenomenon. Methods: Adult female Wistar rats showing diestrus day 1 were treated with: a) ethanol (2 or 4 g/kg body weight (b.w.), ip), b) N-omega-nitro-L-arginine methyl ester (L-NAME), well-known competitive inhibitor of all isoforms of NO synthase (NOS), (30 mg/kg b.w., sc) followed by ethanol (4 g/kg, ip) 3 h later and c) L-NAME (30 mg/kg b.w., sc) followed by saline (ip) 3 h later. Untreated rats were used as controls. Adrenocortical expression of GR was estimated by immunohistochemistry. Results: Strong nuclear GR staining was observed throughout the cortex of control rats. Acute ethanol treatment significantly decreased the expression of GR in the zona fasciculata and zona reticularis. Blockade of NO formation had no influence on this effect of ethanol, whereas L-NAME itself induced significant decline in GR immunoreactivity. Conclusions: Obtained findings are the first to demonstrate localization and distribution of the GR throughout the rat adrenal cortex and to suggest that ethanol as well as endogenous NO may modulate adrenocortical expression of this steroid receptor.", journal = "Pharmacological Reports", title = "Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex", volume = "64", number = "4", pages = "896-901", doi = "10.1016/S1734-1140(12)70884-8" }
Đikić, D., Budec, M., Vranješ-Đurić, S., Todorović, V., Drndarević, N. C., Vignjevic, S.,& Mitrovic, O.. (2012). Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex. in Pharmacological Reports, 64(4), 896-901. https://doi.org/10.1016/S1734-1140(12)70884-8
Đikić D, Budec M, Vranješ-Đurić S, Todorović V, Drndarević NC, Vignjevic S, Mitrovic O. Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex. in Pharmacological Reports. 2012;64(4):896-901. doi:10.1016/S1734-1140(12)70884-8 .
Đikić, Dragoslava, Budec, Mirela, Vranješ-Đurić, Sanja, Todorović, Vera, Drndarević, Neda C., Vignjevic, Sanja, Mitrovic, Olivera, "Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex" in Pharmacological Reports, 64, no. 4 (2012):896-901, https://doi.org/10.1016/S1734-1140(12)70884-8 . .