Effect of Chronic Forced Running on Gene Expression of Catecholamine Biosynthetic Enzymes in Stellate Ganglia of Rats
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Аутори
Gavrilović, LjubicaMandušić, Vesna
Stojiljković, Vesna
Kasapović, Jelena
Stojiljković, S.
Pajović, Snežana B.
Dronjak, Slađana
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Приказ свих података о документуАпстракт
The sympathoneural system has a profound influence on the heart function. Sympathetic neurons are the major contributors to the huge rise of circulating noradrenaline (NA) level in response to stressful stimuli. Treadmill training in rats is forced exercise which has the propensity to induce both psychological and physical stress. The aim of this study is to examine how chronic forced running (CFR) affects the expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT)) and cAMP response element-binding (CREB) in stellate ganglia, as well as the concentrations of catecholamines, adrenocorticotropic hormone (ACTH) and corticosterone (CORT) in the plasma of rats. Also, we investigated how the additional acute immobilization stress changes the mentioned parameters. The rat training program consisted of 12 weeks running on a treadmill (20 m/min, 20 min/day). We found that CFR increases TH and... DBH mRNA and protein levels in stellate ganglia, which is followed by increased NA concentration in the plasma. CFR reduces the level of PNMT m RNA, while the level of PNMT protein remains unchanged in stellate ganglia. The increased expression of TH and DBH genes positively correlates with the expression of CREB in stellate ganglia and with plasma ACTH level, while reduced level of PNMT mRNA in stellate ganglia correlates with reduced plasma CORT level. The additional acute immobilization stress increased gene expression of catecholamine biosynthetic enzymes in stellate ganglia, as well as catecholamines, ACTH and CORT levels in the plasma. The results presented here suggest that the continuous increase of the noradrenaline biosynthetic enzyme expression in stellate ganglia due to CFR may play a role in growing risk of cardiovascular diseases.
Кључне речи:
catecholamine / chronic forced running / immobilization stress / stellate ganglia / qRT-PCRИзвор:
Journal of Biological Regulators and Homeostatic Agents, 2012, 26, 3, 367-377Финансирање / пројекти:
- Ћелијске и молекулске основе малигних и кардиоваскуларних обољења-клиничке импликације (RS-41027)
- Акутни коронарни синдром: истраживање вулнерабилности (плака, крви и миокарда), оптимално лечење и одређивање прогностичких фактора (RS-41022)
- Молекуларни механизми патофизиолошких промена у ћелијама централног нервног система и периферног ткива код сисара (RS-173044)
Колекције
Институција/група
VinčaTY - JOUR AU - Gavrilović, Ljubica AU - Mandušić, Vesna AU - Stojiljković, Vesna AU - Kasapović, Jelena AU - Stojiljković, S. AU - Pajović, Snežana B. AU - Dronjak, Slađana PY - 2012 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5112 AB - The sympathoneural system has a profound influence on the heart function. Sympathetic neurons are the major contributors to the huge rise of circulating noradrenaline (NA) level in response to stressful stimuli. Treadmill training in rats is forced exercise which has the propensity to induce both psychological and physical stress. The aim of this study is to examine how chronic forced running (CFR) affects the expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT)) and cAMP response element-binding (CREB) in stellate ganglia, as well as the concentrations of catecholamines, adrenocorticotropic hormone (ACTH) and corticosterone (CORT) in the plasma of rats. Also, we investigated how the additional acute immobilization stress changes the mentioned parameters. The rat training program consisted of 12 weeks running on a treadmill (20 m/min, 20 min/day). We found that CFR increases TH and DBH mRNA and protein levels in stellate ganglia, which is followed by increased NA concentration in the plasma. CFR reduces the level of PNMT m RNA, while the level of PNMT protein remains unchanged in stellate ganglia. The increased expression of TH and DBH genes positively correlates with the expression of CREB in stellate ganglia and with plasma ACTH level, while reduced level of PNMT mRNA in stellate ganglia correlates with reduced plasma CORT level. The additional acute immobilization stress increased gene expression of catecholamine biosynthetic enzymes in stellate ganglia, as well as catecholamines, ACTH and CORT levels in the plasma. The results presented here suggest that the continuous increase of the noradrenaline biosynthetic enzyme expression in stellate ganglia due to CFR may play a role in growing risk of cardiovascular diseases. T2 - Journal of Biological Regulators and Homeostatic Agents T1 - Effect of Chronic Forced Running on Gene Expression of Catecholamine Biosynthetic Enzymes in Stellate Ganglia of Rats VL - 26 IS - 3 SP - 367 EP - 377 UR - https://hdl.handle.net/21.15107/rcub_vinar_5112 ER -
@article{ author = "Gavrilović, Ljubica and Mandušić, Vesna and Stojiljković, Vesna and Kasapović, Jelena and Stojiljković, S. and Pajović, Snežana B. and Dronjak, Slađana", year = "2012", abstract = "The sympathoneural system has a profound influence on the heart function. Sympathetic neurons are the major contributors to the huge rise of circulating noradrenaline (NA) level in response to stressful stimuli. Treadmill training in rats is forced exercise which has the propensity to induce both psychological and physical stress. The aim of this study is to examine how chronic forced running (CFR) affects the expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT)) and cAMP response element-binding (CREB) in stellate ganglia, as well as the concentrations of catecholamines, adrenocorticotropic hormone (ACTH) and corticosterone (CORT) in the plasma of rats. Also, we investigated how the additional acute immobilization stress changes the mentioned parameters. The rat training program consisted of 12 weeks running on a treadmill (20 m/min, 20 min/day). We found that CFR increases TH and DBH mRNA and protein levels in stellate ganglia, which is followed by increased NA concentration in the plasma. CFR reduces the level of PNMT m RNA, while the level of PNMT protein remains unchanged in stellate ganglia. The increased expression of TH and DBH genes positively correlates with the expression of CREB in stellate ganglia and with plasma ACTH level, while reduced level of PNMT mRNA in stellate ganglia correlates with reduced plasma CORT level. The additional acute immobilization stress increased gene expression of catecholamine biosynthetic enzymes in stellate ganglia, as well as catecholamines, ACTH and CORT levels in the plasma. The results presented here suggest that the continuous increase of the noradrenaline biosynthetic enzyme expression in stellate ganglia due to CFR may play a role in growing risk of cardiovascular diseases.", journal = "Journal of Biological Regulators and Homeostatic Agents", title = "Effect of Chronic Forced Running on Gene Expression of Catecholamine Biosynthetic Enzymes in Stellate Ganglia of Rats", volume = "26", number = "3", pages = "367-377", url = "https://hdl.handle.net/21.15107/rcub_vinar_5112" }
Gavrilović, L., Mandušić, V., Stojiljković, V., Kasapović, J., Stojiljković, S., Pajović, S. B.,& Dronjak, S.. (2012). Effect of Chronic Forced Running on Gene Expression of Catecholamine Biosynthetic Enzymes in Stellate Ganglia of Rats. in Journal of Biological Regulators and Homeostatic Agents, 26(3), 367-377. https://hdl.handle.net/21.15107/rcub_vinar_5112
Gavrilović L, Mandušić V, Stojiljković V, Kasapović J, Stojiljković S, Pajović SB, Dronjak S. Effect of Chronic Forced Running on Gene Expression of Catecholamine Biosynthetic Enzymes in Stellate Ganglia of Rats. in Journal of Biological Regulators and Homeostatic Agents. 2012;26(3):367-377. https://hdl.handle.net/21.15107/rcub_vinar_5112 .
Gavrilović, Ljubica, Mandušić, Vesna, Stojiljković, Vesna, Kasapović, Jelena, Stojiljković, S., Pajović, Snežana B., Dronjak, Slađana, "Effect of Chronic Forced Running on Gene Expression of Catecholamine Biosynthetic Enzymes in Stellate Ganglia of Rats" in Journal of Biological Regulators and Homeostatic Agents, 26, no. 3 (2012):367-377, https://hdl.handle.net/21.15107/rcub_vinar_5112 .