Oxidation of Diazinon for the Sensitive Detection By Cholinesterase-Based Bioanalytical Method
Апстракт
The conversion of diazinon, the model compound for OPs, to more potent AChE inhibitor diazoxon was investigated, using enzyme myeloperoxidase (MPO) as an oxidant. The aim was to enhance its inhibitory power in order to develop rapid, effective and specific acethylcholinesterase (AChE)-based bioanalytical method for its detection in environment. 5-min incubation of 1 x 10(-5) M - 1 x 10(-7) M diazinon with 100 nM MPO increased the degree of AChE inhibition from 80 to 10% due to the formation of diazoxon, compared to the control values for samples containing the same diazinon concentrations in untreated samples. The calibration graph for diazinon detection with AChE assay after incubation with MPO was constructed. While the lower detection limit of diazinon (10% AChE inhibition) before oxidation was ca. 1 x 10(-5) M, the detection limit after oxidation was below 1 x 10(-8) M.
Кључне речи:
diazinon / myeloperoxidase / oxidation / bioanalytics / pesticidesИзвор:
Journal of Environmental Protection and Ecology, 2011, 12, 3, 1168-1173Финансирање / пројекти:
- Истраживања интеракција ензима са токсичним и фармаколошки активним молекулима (RS-MESTD-Basic Research (BR or ON)-172023)
Колекције
Институција/група
VinčaTY - JOUR AU - Lazarević-Pašti, Tamara AU - Vasić, Vesna M. PY - 2011 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4534 AB - The conversion of diazinon, the model compound for OPs, to more potent AChE inhibitor diazoxon was investigated, using enzyme myeloperoxidase (MPO) as an oxidant. The aim was to enhance its inhibitory power in order to develop rapid, effective and specific acethylcholinesterase (AChE)-based bioanalytical method for its detection in environment. 5-min incubation of 1 x 10(-5) M - 1 x 10(-7) M diazinon with 100 nM MPO increased the degree of AChE inhibition from 80 to 10% due to the formation of diazoxon, compared to the control values for samples containing the same diazinon concentrations in untreated samples. The calibration graph for diazinon detection with AChE assay after incubation with MPO was constructed. While the lower detection limit of diazinon (10% AChE inhibition) before oxidation was ca. 1 x 10(-5) M, the detection limit after oxidation was below 1 x 10(-8) M. T2 - Journal of Environmental Protection and Ecology T1 - Oxidation of Diazinon for the Sensitive Detection By Cholinesterase-Based Bioanalytical Method VL - 12 IS - 3 SP - 1168 EP - 1173 UR - https://hdl.handle.net/21.15107/rcub_vinar_4534 ER -
@article{ author = "Lazarević-Pašti, Tamara and Vasić, Vesna M.", year = "2011", abstract = "The conversion of diazinon, the model compound for OPs, to more potent AChE inhibitor diazoxon was investigated, using enzyme myeloperoxidase (MPO) as an oxidant. The aim was to enhance its inhibitory power in order to develop rapid, effective and specific acethylcholinesterase (AChE)-based bioanalytical method for its detection in environment. 5-min incubation of 1 x 10(-5) M - 1 x 10(-7) M diazinon with 100 nM MPO increased the degree of AChE inhibition from 80 to 10% due to the formation of diazoxon, compared to the control values for samples containing the same diazinon concentrations in untreated samples. The calibration graph for diazinon detection with AChE assay after incubation with MPO was constructed. While the lower detection limit of diazinon (10% AChE inhibition) before oxidation was ca. 1 x 10(-5) M, the detection limit after oxidation was below 1 x 10(-8) M.", journal = "Journal of Environmental Protection and Ecology", title = "Oxidation of Diazinon for the Sensitive Detection By Cholinesterase-Based Bioanalytical Method", volume = "12", number = "3", pages = "1168-1173", url = "https://hdl.handle.net/21.15107/rcub_vinar_4534" }
Lazarević-Pašti, T.,& Vasić, V. M.. (2011). Oxidation of Diazinon for the Sensitive Detection By Cholinesterase-Based Bioanalytical Method. in Journal of Environmental Protection and Ecology, 12(3), 1168-1173. https://hdl.handle.net/21.15107/rcub_vinar_4534
Lazarević-Pašti T, Vasić VM. Oxidation of Diazinon for the Sensitive Detection By Cholinesterase-Based Bioanalytical Method. in Journal of Environmental Protection and Ecology. 2011;12(3):1168-1173. https://hdl.handle.net/21.15107/rcub_vinar_4534 .
Lazarević-Pašti, Tamara, Vasić, Vesna M., "Oxidation of Diazinon for the Sensitive Detection By Cholinesterase-Based Bioanalytical Method" in Journal of Environmental Protection and Ecology, 12, no. 3 (2011):1168-1173, https://hdl.handle.net/21.15107/rcub_vinar_4534 .