In vitro evaluation of the genotoxicity of ritodrine and verapamil in human lymphocytes
Само за регистроване кориснике
2011
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to investigate the genotoxic effects of ritodrine and verapamil on human peripheral lymphocytes in vitro using micronucleus (MN) test. Also, fluorescence in situ hybridization (FISH) with a centromeric probe was performed to determine the origin of the induced MN. Cells were treated with 8.4 x 10(-6) M - 25.2 x 10(-4) M concentrations for ritodrine and 0.56-11 x 10(-5) M concentrations for verapamil, separately and combined. The MN frequencies showed increase after all treatments, but the difference between treated cells and untreated controls were found to be statistically significant only in the concentration range from 8.4 x 10(-5) M - 4.5 x 10(-4) M for ritodrine, 1.1 - 3.3 x 10(-5) M for verapamil, and in combined treatment with concentrations 8.4 x 10(-5) M + 1.1 x 10(-5) M for ritodrine and verapamil. The highest tested concentrations of both medicaments showed cytotoxic effect. Both medicaments decreased the nuclear division index (NDI) in tested conce...ntrations. The results of FISH analysis suggest that verapamil, separately or combined with ritodrine, shows to a larger extent aneugenic than clastogenic effect.
Кључне речи:
micronucleus / lymphocytes / in vitro / ritodrine / verapamilИзвор:
Human and Experimental Toxicology, 2011, 30, 5, 398-405Финансирање / пројекти:
- Студија генетички ризичних фактора у репродуктивној и развојној биологији (RS-MESTD-MPN2006-2010-143008)
DOI: 10.1177/0960327110372404
ISSN: 0960-3271
PubMed: 20488853
WoS: 000290398900006
Scopus: 2-s2.0-79955704418
Институција/група
VinčaTY - JOUR AU - Milošević-Đorđević, Olivera AU - Grujičić, Darko V. AU - Joksić, Gordana AU - Marinković, Dragoslav M. PY - 2011 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4306 AB - The aim of this study was to investigate the genotoxic effects of ritodrine and verapamil on human peripheral lymphocytes in vitro using micronucleus (MN) test. Also, fluorescence in situ hybridization (FISH) with a centromeric probe was performed to determine the origin of the induced MN. Cells were treated with 8.4 x 10(-6) M - 25.2 x 10(-4) M concentrations for ritodrine and 0.56-11 x 10(-5) M concentrations for verapamil, separately and combined. The MN frequencies showed increase after all treatments, but the difference between treated cells and untreated controls were found to be statistically significant only in the concentration range from 8.4 x 10(-5) M - 4.5 x 10(-4) M for ritodrine, 1.1 - 3.3 x 10(-5) M for verapamil, and in combined treatment with concentrations 8.4 x 10(-5) M + 1.1 x 10(-5) M for ritodrine and verapamil. The highest tested concentrations of both medicaments showed cytotoxic effect. Both medicaments decreased the nuclear division index (NDI) in tested concentrations. The results of FISH analysis suggest that verapamil, separately or combined with ritodrine, shows to a larger extent aneugenic than clastogenic effect. T2 - Human and Experimental Toxicology T1 - In vitro evaluation of the genotoxicity of ritodrine and verapamil in human lymphocytes VL - 30 IS - 5 SP - 398 EP - 405 DO - 10.1177/0960327110372404 ER -
@article{ author = "Milošević-Đorđević, Olivera and Grujičić, Darko V. and Joksić, Gordana and Marinković, Dragoslav M.", year = "2011", abstract = "The aim of this study was to investigate the genotoxic effects of ritodrine and verapamil on human peripheral lymphocytes in vitro using micronucleus (MN) test. Also, fluorescence in situ hybridization (FISH) with a centromeric probe was performed to determine the origin of the induced MN. Cells were treated with 8.4 x 10(-6) M - 25.2 x 10(-4) M concentrations for ritodrine and 0.56-11 x 10(-5) M concentrations for verapamil, separately and combined. The MN frequencies showed increase after all treatments, but the difference between treated cells and untreated controls were found to be statistically significant only in the concentration range from 8.4 x 10(-5) M - 4.5 x 10(-4) M for ritodrine, 1.1 - 3.3 x 10(-5) M for verapamil, and in combined treatment with concentrations 8.4 x 10(-5) M + 1.1 x 10(-5) M for ritodrine and verapamil. The highest tested concentrations of both medicaments showed cytotoxic effect. Both medicaments decreased the nuclear division index (NDI) in tested concentrations. The results of FISH analysis suggest that verapamil, separately or combined with ritodrine, shows to a larger extent aneugenic than clastogenic effect.", journal = "Human and Experimental Toxicology", title = "In vitro evaluation of the genotoxicity of ritodrine and verapamil in human lymphocytes", volume = "30", number = "5", pages = "398-405", doi = "10.1177/0960327110372404" }
Milošević-Đorđević, O., Grujičić, D. V., Joksić, G.,& Marinković, D. M.. (2011). In vitro evaluation of the genotoxicity of ritodrine and verapamil in human lymphocytes. in Human and Experimental Toxicology, 30(5), 398-405. https://doi.org/10.1177/0960327110372404
Milošević-Đorđević O, Grujičić DV, Joksić G, Marinković DM. In vitro evaluation of the genotoxicity of ritodrine and verapamil in human lymphocytes. in Human and Experimental Toxicology. 2011;30(5):398-405. doi:10.1177/0960327110372404 .
Milošević-Đorđević, Olivera, Grujičić, Darko V., Joksić, Gordana, Marinković, Dragoslav M., "In vitro evaluation of the genotoxicity of ritodrine and verapamil in human lymphocytes" in Human and Experimental Toxicology, 30, no. 5 (2011):398-405, https://doi.org/10.1177/0960327110372404 . .