Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak
Само за регистроване кориснике
2010
Аутори
Petrović, Ivan M.Ristić-Fira, Aleksandra
Todorović, Danijela V.
Korićanac, Lela
Valastro, Lucia
Cirrone, Giuseppe Antonio Pablo
Cuttone, Giacomo
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Purpose: To analyse changes of cell inactivation and proliferation under therapeutic irradiation conditions along the proton spread out Bragg peak (SOBP) with particular emphasis on its distal declining edge. Materials and methods: HTB140 cells were irradiated at four positions: plateau, middle, distal end and distal declining edge of the 62 MeV proton SOBP. Doses ranged from 2-16 Gy. They were normalised in the middle of SOBP and delivered following the axial physical dose profile. Survival, proliferation and cell cycle were assessed seven days after irradiation. Results: Moving from proximal to distal irradiation position surviving fractions at 2 Gy (SF2) decreased from 0.88-0.59. Increased radiosensitivity of the cells was noticed for the doses below 4 Gy, resulting in two gradients of cell inactivation, stronger for lower and weaker for higher doses. Relative biological effectiveness (RBE) increased from 1.68-2.84 at the distal end of SOBP. A further rise of RBE reaching 7.14 was a...t its distal declining edge. Following the axial physical dose profile of SOBP the strongest inactivation was attained at its distal end and was comparable to that at its declining edge. Conclusions: Survival data confirmed very high radioresistance of HTB140 cells. An effect similar to low-dose hyper radiosensitivity (HRS) was observed for order of magnitude larger doses. Better response of cells to protons than to gamma-rays was illustrated by rather high RBE. Strong killing ability at the SOBP distal declining edge was the consequence of increasing proton linear energy transfer.
Кључне речи:
proton spread-out Bragg peak / LET / HTB140 melanoma cells / survival / proliferation / cell cycle analysesИзвор:
International Journal of Radiation Biology, 2010, 86, 9, 742-751Финансирање / пројекти:
- Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, Italy
- Сигнални путеви деловања стероидних хормона и утицај ендогених и егзогених фактора на модулацију процеса у ћелијама сисара (RS-MESTD-MPN2006-2010-143044)
- Експериментална физика високих енергија на детектору CMS у CERN-у (RS-MESTD-MPN2006-2010-141038)
DOI: 10.3109/09553002.2010.481322
ISSN: 0955-3002; 1362-3095
PubMed: 20597839
WoS: 000281771100003
Scopus: 2-s2.0-77956285946
Институција/група
VinčaTY - JOUR AU - Petrović, Ivan M. AU - Ristić-Fira, Aleksandra AU - Todorović, Danijela V. AU - Korićanac, Lela AU - Valastro, Lucia AU - Cirrone, Giuseppe Antonio Pablo AU - Cuttone, Giacomo PY - 2010 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4101 AB - Purpose: To analyse changes of cell inactivation and proliferation under therapeutic irradiation conditions along the proton spread out Bragg peak (SOBP) with particular emphasis on its distal declining edge. Materials and methods: HTB140 cells were irradiated at four positions: plateau, middle, distal end and distal declining edge of the 62 MeV proton SOBP. Doses ranged from 2-16 Gy. They were normalised in the middle of SOBP and delivered following the axial physical dose profile. Survival, proliferation and cell cycle were assessed seven days after irradiation. Results: Moving from proximal to distal irradiation position surviving fractions at 2 Gy (SF2) decreased from 0.88-0.59. Increased radiosensitivity of the cells was noticed for the doses below 4 Gy, resulting in two gradients of cell inactivation, stronger for lower and weaker for higher doses. Relative biological effectiveness (RBE) increased from 1.68-2.84 at the distal end of SOBP. A further rise of RBE reaching 7.14 was at its distal declining edge. Following the axial physical dose profile of SOBP the strongest inactivation was attained at its distal end and was comparable to that at its declining edge. Conclusions: Survival data confirmed very high radioresistance of HTB140 cells. An effect similar to low-dose hyper radiosensitivity (HRS) was observed for order of magnitude larger doses. Better response of cells to protons than to gamma-rays was illustrated by rather high RBE. Strong killing ability at the SOBP distal declining edge was the consequence of increasing proton linear energy transfer. T2 - International Journal of Radiation Biology T1 - Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak VL - 86 IS - 9 SP - 742 EP - 751 DO - 10.3109/09553002.2010.481322 ER -
@article{ author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V. and Korićanac, Lela and Valastro, Lucia and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo", year = "2010", abstract = "Purpose: To analyse changes of cell inactivation and proliferation under therapeutic irradiation conditions along the proton spread out Bragg peak (SOBP) with particular emphasis on its distal declining edge. Materials and methods: HTB140 cells were irradiated at four positions: plateau, middle, distal end and distal declining edge of the 62 MeV proton SOBP. Doses ranged from 2-16 Gy. They were normalised in the middle of SOBP and delivered following the axial physical dose profile. Survival, proliferation and cell cycle were assessed seven days after irradiation. Results: Moving from proximal to distal irradiation position surviving fractions at 2 Gy (SF2) decreased from 0.88-0.59. Increased radiosensitivity of the cells was noticed for the doses below 4 Gy, resulting in two gradients of cell inactivation, stronger for lower and weaker for higher doses. Relative biological effectiveness (RBE) increased from 1.68-2.84 at the distal end of SOBP. A further rise of RBE reaching 7.14 was at its distal declining edge. Following the axial physical dose profile of SOBP the strongest inactivation was attained at its distal end and was comparable to that at its declining edge. Conclusions: Survival data confirmed very high radioresistance of HTB140 cells. An effect similar to low-dose hyper radiosensitivity (HRS) was observed for order of magnitude larger doses. Better response of cells to protons than to gamma-rays was illustrated by rather high RBE. Strong killing ability at the SOBP distal declining edge was the consequence of increasing proton linear energy transfer.", journal = "International Journal of Radiation Biology", title = "Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak", volume = "86", number = "9", pages = "742-751", doi = "10.3109/09553002.2010.481322" }
Petrović, I. M., Ristić-Fira, A., Todorović, D. V., Korićanac, L., Valastro, L., Cirrone, G. A. P.,& Cuttone, G.. (2010). Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak. in International Journal of Radiation Biology, 86(9), 742-751. https://doi.org/10.3109/09553002.2010.481322
Petrović IM, Ristić-Fira A, Todorović DV, Korićanac L, Valastro L, Cirrone GAP, Cuttone G. Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak. in International Journal of Radiation Biology. 2010;86(9):742-751. doi:10.3109/09553002.2010.481322 .
Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., Korićanac, Lela, Valastro, Lucia, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, "Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak" in International Journal of Radiation Biology, 86, no. 9 (2010):742-751, https://doi.org/10.3109/09553002.2010.481322 . .