Sexually dimorphic functional alterations of rat hepatic glucocorticoid receptor in response to fluoxetine
Само за регистроване кориснике
2010
Аутори
Elaković, IvanaVasiljević, Đorđe
Adžić, Miroslav
Đorđević, Ana D.
Đorđević, Jelena D.
Matić, Gordana
Radojčić, Marija
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Gender-related differences in the expression and functional properties of the hepatic glucocorticoid receptor were studied before and after antidepressant fluoxetine administration to both unstressed and rats exposed to a chronic social isolation stress. Some of the receptors functional properties, including hormone-binding capacity (B-max), hormone-binding potency (B-max/K-D ratio) and the DNA-binding ability, were found to be sexually dimorphic. Fluoxetine treatment (5 mg/kg body mass, 21 day, intraperitoneally) induced a decrease in B-max and in the amount of Hsp70 co-immunoprecipitated with the glucocorticoid receptor only in males, and stimulated the association of the receptor with Hsp90 in females. When applied during the last three weeks of the 6-week isolation, fluoxetine parallelly elevated B-max and the receptor protein level in female animals, while in males diminished B-max and inhibited association of the receptor with Hsp70. Binding of dexamethasone-receptor complexes bo...th to DNA-cellulose and to isolated liver nuclei did not appear to be a target for fluoxetine action. The results point to sex-related differences in the glucocorticoid receptor functioning and in its response to fluoxetine, and suggest that these differences may contribute to well known sexual dimorphism in the sensitivity to stress, to stress-related disorders and to antidepressant treatment. (C) 2010 Elsevier B.V. All rights reserved.
Кључне речи:
Glucocorticoid receptor / Gender differences / Heat shock proteins / Antidepressant fluoxetine / Isolation stress / Rat liverИзвор:
European Journal of Pharmacology, 2010, 632, 1-3, 79-85Финансирање / пројекти:
- Експресија и функција глукокортикоидног рецептора и протеина топлотног стреса у патофизиолошким стањима и стресу (RS-MESTD-MPN2006-2010-143003)
- Ministry of Science of the Republic of Serbia [143042B]
DOI: 10.1016/j.ejphar.2010.01.015
ISSN: 0014-2999; 1879-0712
PubMed: 20122922
WoS: 000275941900012
Scopus: 2-s2.0-77549087261
Колекције
Институција/група
VinčaTY - JOUR AU - Elaković, Ivana AU - Vasiljević, Đorđe AU - Adžić, Miroslav AU - Đorđević, Ana D. AU - Đorđević, Jelena D. AU - Matić, Gordana AU - Radojčić, Marija PY - 2010 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3960 AB - Gender-related differences in the expression and functional properties of the hepatic glucocorticoid receptor were studied before and after antidepressant fluoxetine administration to both unstressed and rats exposed to a chronic social isolation stress. Some of the receptors functional properties, including hormone-binding capacity (B-max), hormone-binding potency (B-max/K-D ratio) and the DNA-binding ability, were found to be sexually dimorphic. Fluoxetine treatment (5 mg/kg body mass, 21 day, intraperitoneally) induced a decrease in B-max and in the amount of Hsp70 co-immunoprecipitated with the glucocorticoid receptor only in males, and stimulated the association of the receptor with Hsp90 in females. When applied during the last three weeks of the 6-week isolation, fluoxetine parallelly elevated B-max and the receptor protein level in female animals, while in males diminished B-max and inhibited association of the receptor with Hsp70. Binding of dexamethasone-receptor complexes both to DNA-cellulose and to isolated liver nuclei did not appear to be a target for fluoxetine action. The results point to sex-related differences in the glucocorticoid receptor functioning and in its response to fluoxetine, and suggest that these differences may contribute to well known sexual dimorphism in the sensitivity to stress, to stress-related disorders and to antidepressant treatment. (C) 2010 Elsevier B.V. All rights reserved. T2 - European Journal of Pharmacology T1 - Sexually dimorphic functional alterations of rat hepatic glucocorticoid receptor in response to fluoxetine VL - 632 IS - 1-3 SP - 79 EP - 85 DO - 10.1016/j.ejphar.2010.01.015 ER -
@article{ author = "Elaković, Ivana and Vasiljević, Đorđe and Adžić, Miroslav and Đorđević, Ana D. and Đorđević, Jelena D. and Matić, Gordana and Radojčić, Marija", year = "2010", abstract = "Gender-related differences in the expression and functional properties of the hepatic glucocorticoid receptor were studied before and after antidepressant fluoxetine administration to both unstressed and rats exposed to a chronic social isolation stress. Some of the receptors functional properties, including hormone-binding capacity (B-max), hormone-binding potency (B-max/K-D ratio) and the DNA-binding ability, were found to be sexually dimorphic. Fluoxetine treatment (5 mg/kg body mass, 21 day, intraperitoneally) induced a decrease in B-max and in the amount of Hsp70 co-immunoprecipitated with the glucocorticoid receptor only in males, and stimulated the association of the receptor with Hsp90 in females. When applied during the last three weeks of the 6-week isolation, fluoxetine parallelly elevated B-max and the receptor protein level in female animals, while in males diminished B-max and inhibited association of the receptor with Hsp70. Binding of dexamethasone-receptor complexes both to DNA-cellulose and to isolated liver nuclei did not appear to be a target for fluoxetine action. The results point to sex-related differences in the glucocorticoid receptor functioning and in its response to fluoxetine, and suggest that these differences may contribute to well known sexual dimorphism in the sensitivity to stress, to stress-related disorders and to antidepressant treatment. (C) 2010 Elsevier B.V. All rights reserved.", journal = "European Journal of Pharmacology", title = "Sexually dimorphic functional alterations of rat hepatic glucocorticoid receptor in response to fluoxetine", volume = "632", number = "1-3", pages = "79-85", doi = "10.1016/j.ejphar.2010.01.015" }
Elaković, I., Vasiljević, Đ., Adžić, M., Đorđević, A. D., Đorđević, J. D., Matić, G.,& Radojčić, M.. (2010). Sexually dimorphic functional alterations of rat hepatic glucocorticoid receptor in response to fluoxetine. in European Journal of Pharmacology, 632(1-3), 79-85. https://doi.org/10.1016/j.ejphar.2010.01.015
Elaković I, Vasiljević Đ, Adžić M, Đorđević AD, Đorđević JD, Matić G, Radojčić M. Sexually dimorphic functional alterations of rat hepatic glucocorticoid receptor in response to fluoxetine. in European Journal of Pharmacology. 2010;632(1-3):79-85. doi:10.1016/j.ejphar.2010.01.015 .
Elaković, Ivana, Vasiljević, Đorđe, Adžić, Miroslav, Đorđević, Ana D., Đorđević, Jelena D., Matić, Gordana, Radojčić, Marija, "Sexually dimorphic functional alterations of rat hepatic glucocorticoid receptor in response to fluoxetine" in European Journal of Pharmacology, 632, no. 1-3 (2010):79-85, https://doi.org/10.1016/j.ejphar.2010.01.015 . .