Brain Injury Alters Ectonucleotidase Activities and Adenine Nucleotide Levels in Rat Serum
2015
Аутори
Laketa, DanijelaSavić, Jasmina
Bjelobaba, Ivana
Lavrnja, Irene
Vasić, Vesna M.
Stojiljković, Mirjana
Nedeljković, Nadežda
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background: Cortical stab injury (CSI) induces changes in the activity, expression and cellular distribution of specific ectonucleotidases at the injury site. Also, several experimentally induced neuropathologies are associated with changes in soluble ectonucleotidase activities in the plasma and serum, whilst various insults to the brain alter purine compounds levels in cerebrospinal fluid, but also in serum, indicating that insults to the brain may induce alterations in nucleotides release and rate of their hydrolysis in the vascular system. Since adenine nucleotides and adenosine regulate diverse cellular functions in the vascular system, including vascular tone, platelet aggregation and inflammatory responses of lymphocytes and macrophages, alterations of ectonucleotidase activities in the vascular system may be relevant for the clinical outcome of the primary insult. Methods: We explored ectonucleotidase activities using specific enzyme assays and determined adenine nucleotides co...ncentrations by the UPLC method in the rat serum after cortical stab injury. Results: At 4-h post-injury, ATP and AMP hydrolysis increased by about 60% and 40%, respectively, while phosphodiesterase activity remained unchanged. Also, at 4-h postinjury a marked decrease in ATP concentration and more than 2-fold increase in AMP concentration were recorded. Conclusions: CSI induces rapid up-regulation of nucleotide catabolizing soluble ectonucleotidases in rat serum, which leads to the observed shift in serum nucleotide levels. The results obtained imply that ectonucleotidases and adenine nucleotides participate in the communication between the brain and the vascular system in physiological and pathological conditions and thereby may be involved in the development of various human neuropathologies.
Кључне речи:
ectonucleotidases / extracellular adenine nucleotides / ATP / brain injury / ratИзвор:
Journal of Medical Biochemistry, 2015, 34, 2, 215-222Финансирање / пројекти:
- Истраживања интеракција ензима са токсичним и фармаколошки активним молекулима (RS-MESTD-Basic Research (BR or ON)-172023)
- Ћелијска и молекулска основа неуроинфламације: потенцијала циљна места за транслациону медицину и терапију (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41014)
DOI: 10.2478/jomb-2014-0025
ISSN: 1452-8258; 1452-8266
PubMed: 28356834
WoS: 000348895300008
Scopus: 2-s2.0-84925633777
Колекције
Институција/група
VinčaTY - JOUR AU - Laketa, Danijela AU - Savić, Jasmina AU - Bjelobaba, Ivana AU - Lavrnja, Irene AU - Vasić, Vesna M. AU - Stojiljković, Mirjana AU - Nedeljković, Nadežda PY - 2015 UR - https://vinar.vin.bg.ac.rs/handle/123456789/385 AB - Background: Cortical stab injury (CSI) induces changes in the activity, expression and cellular distribution of specific ectonucleotidases at the injury site. Also, several experimentally induced neuropathologies are associated with changes in soluble ectonucleotidase activities in the plasma and serum, whilst various insults to the brain alter purine compounds levels in cerebrospinal fluid, but also in serum, indicating that insults to the brain may induce alterations in nucleotides release and rate of their hydrolysis in the vascular system. Since adenine nucleotides and adenosine regulate diverse cellular functions in the vascular system, including vascular tone, platelet aggregation and inflammatory responses of lymphocytes and macrophages, alterations of ectonucleotidase activities in the vascular system may be relevant for the clinical outcome of the primary insult. Methods: We explored ectonucleotidase activities using specific enzyme assays and determined adenine nucleotides concentrations by the UPLC method in the rat serum after cortical stab injury. Results: At 4-h post-injury, ATP and AMP hydrolysis increased by about 60% and 40%, respectively, while phosphodiesterase activity remained unchanged. Also, at 4-h postinjury a marked decrease in ATP concentration and more than 2-fold increase in AMP concentration were recorded. Conclusions: CSI induces rapid up-regulation of nucleotide catabolizing soluble ectonucleotidases in rat serum, which leads to the observed shift in serum nucleotide levels. The results obtained imply that ectonucleotidases and adenine nucleotides participate in the communication between the brain and the vascular system in physiological and pathological conditions and thereby may be involved in the development of various human neuropathologies. T2 - Journal of Medical Biochemistry T1 - Brain Injury Alters Ectonucleotidase Activities and Adenine Nucleotide Levels in Rat Serum VL - 34 IS - 2 SP - 215 EP - 222 DO - 10.2478/jomb-2014-0025 ER -
@article{ author = "Laketa, Danijela and Savić, Jasmina and Bjelobaba, Ivana and Lavrnja, Irene and Vasić, Vesna M. and Stojiljković, Mirjana and Nedeljković, Nadežda", year = "2015", abstract = "Background: Cortical stab injury (CSI) induces changes in the activity, expression and cellular distribution of specific ectonucleotidases at the injury site. Also, several experimentally induced neuropathologies are associated with changes in soluble ectonucleotidase activities in the plasma and serum, whilst various insults to the brain alter purine compounds levels in cerebrospinal fluid, but also in serum, indicating that insults to the brain may induce alterations in nucleotides release and rate of their hydrolysis in the vascular system. Since adenine nucleotides and adenosine regulate diverse cellular functions in the vascular system, including vascular tone, platelet aggregation and inflammatory responses of lymphocytes and macrophages, alterations of ectonucleotidase activities in the vascular system may be relevant for the clinical outcome of the primary insult. Methods: We explored ectonucleotidase activities using specific enzyme assays and determined adenine nucleotides concentrations by the UPLC method in the rat serum after cortical stab injury. Results: At 4-h post-injury, ATP and AMP hydrolysis increased by about 60% and 40%, respectively, while phosphodiesterase activity remained unchanged. Also, at 4-h postinjury a marked decrease in ATP concentration and more than 2-fold increase in AMP concentration were recorded. Conclusions: CSI induces rapid up-regulation of nucleotide catabolizing soluble ectonucleotidases in rat serum, which leads to the observed shift in serum nucleotide levels. The results obtained imply that ectonucleotidases and adenine nucleotides participate in the communication between the brain and the vascular system in physiological and pathological conditions and thereby may be involved in the development of various human neuropathologies.", journal = "Journal of Medical Biochemistry", title = "Brain Injury Alters Ectonucleotidase Activities and Adenine Nucleotide Levels in Rat Serum", volume = "34", number = "2", pages = "215-222", doi = "10.2478/jomb-2014-0025" }
Laketa, D., Savić, J., Bjelobaba, I., Lavrnja, I., Vasić, V. M., Stojiljković, M.,& Nedeljković, N.. (2015). Brain Injury Alters Ectonucleotidase Activities and Adenine Nucleotide Levels in Rat Serum. in Journal of Medical Biochemistry, 34(2), 215-222. https://doi.org/10.2478/jomb-2014-0025
Laketa D, Savić J, Bjelobaba I, Lavrnja I, Vasić VM, Stojiljković M, Nedeljković N. Brain Injury Alters Ectonucleotidase Activities and Adenine Nucleotide Levels in Rat Serum. in Journal of Medical Biochemistry. 2015;34(2):215-222. doi:10.2478/jomb-2014-0025 .
Laketa, Danijela, Savić, Jasmina, Bjelobaba, Ivana, Lavrnja, Irene, Vasić, Vesna M., Stojiljković, Mirjana, Nedeljković, Nadežda, "Brain Injury Alters Ectonucleotidase Activities and Adenine Nucleotide Levels in Rat Serum" in Journal of Medical Biochemistry, 34, no. 2 (2015):215-222, https://doi.org/10.2478/jomb-2014-0025 . .