Rosiglitazone modulates insulin-induced plasma membrane area changes in single 3T3-L1 adipocytes
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Аутори
Velebit, JelenaKovacic, Petra Brina
Prebil, Mateja
Chowdhury, Helena H.
Grilc, Sonja
Kreft, Marko
Jensen, Jorgen
Isenović, Esma R.
Zorec, Robert
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In this study we hypothesized that rosiglitazone, an antidiabetic high-affinity agonist for the peroxisome proliferator-activated receptor gamma, affects the plasma membrane (PM) turnover in single 3T3-L1 adipocytes. To study the PM turnover, the patch-clamp electrophysiological method was used to measure changes in membrane capacitance (C-m), a parameter linearly related to the PM area. Microscopy results show that the presence of rosiglitazone in the differentiating medium significantly increased the differentiation of 3T3-L1 adipocytes in cell culture, based on oil red O-stained area (11.4 +/- 1.2%) vs. controls (3.1 +/- 0.5%). Moreover, rosiglitazone treatment significantly reduced the size of single 3T3-L1 adipocytes; their average radius of 21.1 +/- 1.1 mu m in controls was reduced to 17.5 +/- 0.5 mu m in rosiglitazone-treated cells. Consistent with this, insulin application increased the rate of C-m increase to 2.34 +/- 0.10%/min, which was significantly different from controls ...(0.12 +/- 0.08%/min). However, pretreatment of cells with rosiglitazone prior to the treatment with insulin resulted in an attenuated rate of C (m) increase. These data support the involvement of insulin in the modulation of membrane area and show that treatment by rosiglitazone reduced the insulin-mediated membrane area increase in 3T3-L1 adipocytes.
Кључне речи:
3T3-L1 adipocyte / insulin / rosiglitazone / exocytosis / endocytosis / membrane area regulationИзвор:
Journal of Membrane Biology, 2008, 223, 3, 141-149Финансирање / пројекти:
- Ministry of Higher Education, Science and Technology of the Republic of Slovenia [P3 310 381], Ministry of Science and Environmental Protection, Republic of Serbia [143030B], Republic of Slovenia and the Republic of Serbia [BI-CS/06-07-006], COST B17
DOI: 10.1007/s00232-008-9120-x
ISSN: 0022-2631
PubMed: 18754069
WoS: 000259733600003
Scopus: 2-s2.0-53849137995
Колекције
Институција/група
VinčaTY - JOUR AU - Velebit, Jelena AU - Kovacic, Petra Brina AU - Prebil, Mateja AU - Chowdhury, Helena H. AU - Grilc, Sonja AU - Kreft, Marko AU - Jensen, Jorgen AU - Isenović, Esma R. AU - Zorec, Robert PY - 2008 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3537 AB - In this study we hypothesized that rosiglitazone, an antidiabetic high-affinity agonist for the peroxisome proliferator-activated receptor gamma, affects the plasma membrane (PM) turnover in single 3T3-L1 adipocytes. To study the PM turnover, the patch-clamp electrophysiological method was used to measure changes in membrane capacitance (C-m), a parameter linearly related to the PM area. Microscopy results show that the presence of rosiglitazone in the differentiating medium significantly increased the differentiation of 3T3-L1 adipocytes in cell culture, based on oil red O-stained area (11.4 +/- 1.2%) vs. controls (3.1 +/- 0.5%). Moreover, rosiglitazone treatment significantly reduced the size of single 3T3-L1 adipocytes; their average radius of 21.1 +/- 1.1 mu m in controls was reduced to 17.5 +/- 0.5 mu m in rosiglitazone-treated cells. Consistent with this, insulin application increased the rate of C-m increase to 2.34 +/- 0.10%/min, which was significantly different from controls (0.12 +/- 0.08%/min). However, pretreatment of cells with rosiglitazone prior to the treatment with insulin resulted in an attenuated rate of C (m) increase. These data support the involvement of insulin in the modulation of membrane area and show that treatment by rosiglitazone reduced the insulin-mediated membrane area increase in 3T3-L1 adipocytes. T2 - Journal of Membrane Biology T1 - Rosiglitazone modulates insulin-induced plasma membrane area changes in single 3T3-L1 adipocytes VL - 223 IS - 3 SP - 141 EP - 149 DO - 10.1007/s00232-008-9120-x ER -
@article{ author = "Velebit, Jelena and Kovacic, Petra Brina and Prebil, Mateja and Chowdhury, Helena H. and Grilc, Sonja and Kreft, Marko and Jensen, Jorgen and Isenović, Esma R. and Zorec, Robert", year = "2008", abstract = "In this study we hypothesized that rosiglitazone, an antidiabetic high-affinity agonist for the peroxisome proliferator-activated receptor gamma, affects the plasma membrane (PM) turnover in single 3T3-L1 adipocytes. To study the PM turnover, the patch-clamp electrophysiological method was used to measure changes in membrane capacitance (C-m), a parameter linearly related to the PM area. Microscopy results show that the presence of rosiglitazone in the differentiating medium significantly increased the differentiation of 3T3-L1 adipocytes in cell culture, based on oil red O-stained area (11.4 +/- 1.2%) vs. controls (3.1 +/- 0.5%). Moreover, rosiglitazone treatment significantly reduced the size of single 3T3-L1 adipocytes; their average radius of 21.1 +/- 1.1 mu m in controls was reduced to 17.5 +/- 0.5 mu m in rosiglitazone-treated cells. Consistent with this, insulin application increased the rate of C-m increase to 2.34 +/- 0.10%/min, which was significantly different from controls (0.12 +/- 0.08%/min). However, pretreatment of cells with rosiglitazone prior to the treatment with insulin resulted in an attenuated rate of C (m) increase. These data support the involvement of insulin in the modulation of membrane area and show that treatment by rosiglitazone reduced the insulin-mediated membrane area increase in 3T3-L1 adipocytes.", journal = "Journal of Membrane Biology", title = "Rosiglitazone modulates insulin-induced plasma membrane area changes in single 3T3-L1 adipocytes", volume = "223", number = "3", pages = "141-149", doi = "10.1007/s00232-008-9120-x" }
Velebit, J., Kovacic, P. B., Prebil, M., Chowdhury, H. H., Grilc, S., Kreft, M., Jensen, J., Isenović, E. R.,& Zorec, R.. (2008). Rosiglitazone modulates insulin-induced plasma membrane area changes in single 3T3-L1 adipocytes. in Journal of Membrane Biology, 223(3), 141-149. https://doi.org/10.1007/s00232-008-9120-x
Velebit J, Kovacic PB, Prebil M, Chowdhury HH, Grilc S, Kreft M, Jensen J, Isenović ER, Zorec R. Rosiglitazone modulates insulin-induced plasma membrane area changes in single 3T3-L1 adipocytes. in Journal of Membrane Biology. 2008;223(3):141-149. doi:10.1007/s00232-008-9120-x .
Velebit, Jelena, Kovacic, Petra Brina, Prebil, Mateja, Chowdhury, Helena H., Grilc, Sonja, Kreft, Marko, Jensen, Jorgen, Isenović, Esma R., Zorec, Robert, "Rosiglitazone modulates insulin-induced plasma membrane area changes in single 3T3-L1 adipocytes" in Journal of Membrane Biology, 223, no. 3 (2008):141-149, https://doi.org/10.1007/s00232-008-9120-x . .