Insulin signaling in the liver and uterus of ovariectomized rats treated with estradiol
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Аутори
Korićanac, GoranMilosavljević, Tijana
Stojiljković, Mojca D.
Žakula, Zorica
Ribarac-Stepić, Nevena B.
Isenović, Esma R.
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We used rat hepatic and uterine tissues to examine the impact of estradiol (E2) on insulin (INS) signaling. Ovariectotnized (OVX) female Wistar rats were treated with E2 (20 mu g/kg b.wt., i.p.) and used for the experiment 6 h after E2 administration. To highlight E2 effects on tyrosine phosphorylation of INS receptor (IR) and INS receptor substrates (IRSs) and IRSs association with p85 subunit of phosphatidylinositol 3-kinase (PI3-K) in the context of INS signaling, E2-treated OVX rats were also injected with INS (20 IU, i.p.), 30 min before the experiment. Treatment with E2 did not change the levels of plasma INS and glucose (Glu). However, it significantly decreased the free fatty acid (FFA) level and increased uterine weight. Furthermore, the results show that E2 had no effect on the content of hepatic IR protein, but significantly increased IR protein content in the uterus and decreased IR tyrosine phosphorylation in both the liver and uterus. Compared to the control, hepatic IRS-...1 and IRS-2 were significantly decreased and increased, respectively, after E2 treatment. Protein content of both molecules, IRS-1 and IRS-2, was increased in uterine tissue after E2 administration. Protein content of the p85 subunit of PI3-K and that of protein kinase B (Akt) were increased in the uterus, with no changes in the liver. The results suggest that E2 treatment induces tissue-specific changes in INS signaling. The consequences of E2 treatment on INS signaling molecules are more apparent in the uterus, but their physiological relevance for INS action is probably greater in the liver. (c) 2007 Elsevier Ltd. All rights reserved.
Кључне речи:
estradiol / insulin signaling / liver / uterusИзвор:
Journal of Steroid Biochemistry and Molecular Biology, 2008, 108, 1-2, 109-116
DOI: 10.1016/j.jsbmb.2007.06.001
ISSN: 0960-0760
PubMed: 17931855
WoS: 000253824900013
Scopus: 2-s2.0-38849204979
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Институција/група
VinčaTY - JOUR AU - Korićanac, Goran AU - Milosavljević, Tijana AU - Stojiljković, Mojca D. AU - Žakula, Zorica AU - Ribarac-Stepić, Nevena B. AU - Isenović, Esma R. PY - 2008 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3378 AB - We used rat hepatic and uterine tissues to examine the impact of estradiol (E2) on insulin (INS) signaling. Ovariectotnized (OVX) female Wistar rats were treated with E2 (20 mu g/kg b.wt., i.p.) and used for the experiment 6 h after E2 administration. To highlight E2 effects on tyrosine phosphorylation of INS receptor (IR) and INS receptor substrates (IRSs) and IRSs association with p85 subunit of phosphatidylinositol 3-kinase (PI3-K) in the context of INS signaling, E2-treated OVX rats were also injected with INS (20 IU, i.p.), 30 min before the experiment. Treatment with E2 did not change the levels of plasma INS and glucose (Glu). However, it significantly decreased the free fatty acid (FFA) level and increased uterine weight. Furthermore, the results show that E2 had no effect on the content of hepatic IR protein, but significantly increased IR protein content in the uterus and decreased IR tyrosine phosphorylation in both the liver and uterus. Compared to the control, hepatic IRS-1 and IRS-2 were significantly decreased and increased, respectively, after E2 treatment. Protein content of both molecules, IRS-1 and IRS-2, was increased in uterine tissue after E2 administration. Protein content of the p85 subunit of PI3-K and that of protein kinase B (Akt) were increased in the uterus, with no changes in the liver. The results suggest that E2 treatment induces tissue-specific changes in INS signaling. The consequences of E2 treatment on INS signaling molecules are more apparent in the uterus, but their physiological relevance for INS action is probably greater in the liver. (c) 2007 Elsevier Ltd. All rights reserved. T2 - Journal of Steroid Biochemistry and Molecular Biology T1 - Insulin signaling in the liver and uterus of ovariectomized rats treated with estradiol VL - 108 IS - 1-2 SP - 109 EP - 116 DO - 10.1016/j.jsbmb.2007.06.001 ER -
@article{ author = "Korićanac, Goran and Milosavljević, Tijana and Stojiljković, Mojca D. and Žakula, Zorica and Ribarac-Stepić, Nevena B. and Isenović, Esma R.", year = "2008", abstract = "We used rat hepatic and uterine tissues to examine the impact of estradiol (E2) on insulin (INS) signaling. Ovariectotnized (OVX) female Wistar rats were treated with E2 (20 mu g/kg b.wt., i.p.) and used for the experiment 6 h after E2 administration. To highlight E2 effects on tyrosine phosphorylation of INS receptor (IR) and INS receptor substrates (IRSs) and IRSs association with p85 subunit of phosphatidylinositol 3-kinase (PI3-K) in the context of INS signaling, E2-treated OVX rats were also injected with INS (20 IU, i.p.), 30 min before the experiment. Treatment with E2 did not change the levels of plasma INS and glucose (Glu). However, it significantly decreased the free fatty acid (FFA) level and increased uterine weight. Furthermore, the results show that E2 had no effect on the content of hepatic IR protein, but significantly increased IR protein content in the uterus and decreased IR tyrosine phosphorylation in both the liver and uterus. Compared to the control, hepatic IRS-1 and IRS-2 were significantly decreased and increased, respectively, after E2 treatment. Protein content of both molecules, IRS-1 and IRS-2, was increased in uterine tissue after E2 administration. Protein content of the p85 subunit of PI3-K and that of protein kinase B (Akt) were increased in the uterus, with no changes in the liver. The results suggest that E2 treatment induces tissue-specific changes in INS signaling. The consequences of E2 treatment on INS signaling molecules are more apparent in the uterus, but their physiological relevance for INS action is probably greater in the liver. (c) 2007 Elsevier Ltd. All rights reserved.", journal = "Journal of Steroid Biochemistry and Molecular Biology", title = "Insulin signaling in the liver and uterus of ovariectomized rats treated with estradiol", volume = "108", number = "1-2", pages = "109-116", doi = "10.1016/j.jsbmb.2007.06.001" }
Korićanac, G., Milosavljević, T., Stojiljković, M. D., Žakula, Z., Ribarac-Stepić, N. B.,& Isenović, E. R.. (2008). Insulin signaling in the liver and uterus of ovariectomized rats treated with estradiol. in Journal of Steroid Biochemistry and Molecular Biology, 108(1-2), 109-116. https://doi.org/10.1016/j.jsbmb.2007.06.001
Korićanac G, Milosavljević T, Stojiljković MD, Žakula Z, Ribarac-Stepić NB, Isenović ER. Insulin signaling in the liver and uterus of ovariectomized rats treated with estradiol. in Journal of Steroid Biochemistry and Molecular Biology. 2008;108(1-2):109-116. doi:10.1016/j.jsbmb.2007.06.001 .
Korićanac, Goran, Milosavljević, Tijana, Stojiljković, Mojca D., Žakula, Zorica, Ribarac-Stepić, Nevena B., Isenović, Esma R., "Insulin signaling in the liver and uterus of ovariectomized rats treated with estradiol" in Journal of Steroid Biochemistry and Molecular Biology, 108, no. 1-2 (2008):109-116, https://doi.org/10.1016/j.jsbmb.2007.06.001 . .