DNA profiling of B chromosomes from the yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia)
Апстракт
Using AP-PCR-based DNA profiling we examined some structural features of B chromosomes from yellow-necked mice Apodemus flavicollis. Mice harboring one,two, or three or lacking B chromosomes were examined. Chromosomal structure was scanned for variant bands by using a series of arbitrary primers and From these, informative bands were selected. The selection:criteria used were the ability to differentiate between individuals of the species, to detect markers common For both A and B chromosomes, and, importantly, to differentiate between A- and B-chromosome sets. In addition to primers, profiling conditions were found to be critical for meeting the selection criteria. Primers and analysis conditions that demonstrated structural characteristics unique to the B-chromosome set are described. These characteristics included variant bands as qualitative parameters and altered electrophoretic band-intensities as quantitative distinctions estimated by integration of densitometric profiles of ele...ctrophoretograms. B chromosome-specific molecular markers are easy to detect by AP-PCR-based DNA profiling in the presence of a full set of A chromosomes. Models for the origin of yellow-necked mouse B chromosomes are discussed in the context of presented data.
Извор:
Genome Research, 2000, 10, 1, 55-61Колекције
Институција/група
VinčaTY - JOUR AU - Tanić, Nikola AU - Dedović-Tanić, Nasta AU - Vujošević, Mladen AU - Dimitrijević, Bogomir B. PY - 2000 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2325 AB - Using AP-PCR-based DNA profiling we examined some structural features of B chromosomes from yellow-necked mice Apodemus flavicollis. Mice harboring one,two, or three or lacking B chromosomes were examined. Chromosomal structure was scanned for variant bands by using a series of arbitrary primers and From these, informative bands were selected. The selection:criteria used were the ability to differentiate between individuals of the species, to detect markers common For both A and B chromosomes, and, importantly, to differentiate between A- and B-chromosome sets. In addition to primers, profiling conditions were found to be critical for meeting the selection criteria. Primers and analysis conditions that demonstrated structural characteristics unique to the B-chromosome set are described. These characteristics included variant bands as qualitative parameters and altered electrophoretic band-intensities as quantitative distinctions estimated by integration of densitometric profiles of electrophoretograms. B chromosome-specific molecular markers are easy to detect by AP-PCR-based DNA profiling in the presence of a full set of A chromosomes. Models for the origin of yellow-necked mouse B chromosomes are discussed in the context of presented data. T2 - Genome Research T1 - DNA profiling of B chromosomes from the yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia) VL - 10 IS - 1 SP - 55 EP - 61 UR - https://hdl.handle.net/21.15107/rcub_vinar_2325 ER -
@article{ author = "Tanić, Nikola and Dedović-Tanić, Nasta and Vujošević, Mladen and Dimitrijević, Bogomir B.", year = "2000", abstract = "Using AP-PCR-based DNA profiling we examined some structural features of B chromosomes from yellow-necked mice Apodemus flavicollis. Mice harboring one,two, or three or lacking B chromosomes were examined. Chromosomal structure was scanned for variant bands by using a series of arbitrary primers and From these, informative bands were selected. The selection:criteria used were the ability to differentiate between individuals of the species, to detect markers common For both A and B chromosomes, and, importantly, to differentiate between A- and B-chromosome sets. In addition to primers, profiling conditions were found to be critical for meeting the selection criteria. Primers and analysis conditions that demonstrated structural characteristics unique to the B-chromosome set are described. These characteristics included variant bands as qualitative parameters and altered electrophoretic band-intensities as quantitative distinctions estimated by integration of densitometric profiles of electrophoretograms. B chromosome-specific molecular markers are easy to detect by AP-PCR-based DNA profiling in the presence of a full set of A chromosomes. Models for the origin of yellow-necked mouse B chromosomes are discussed in the context of presented data.", journal = "Genome Research", title = "DNA profiling of B chromosomes from the yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia)", volume = "10", number = "1", pages = "55-61", url = "https://hdl.handle.net/21.15107/rcub_vinar_2325" }
Tanić, N., Dedović-Tanić, N., Vujošević, M.,& Dimitrijević, B. B.. (2000). DNA profiling of B chromosomes from the yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia). in Genome Research, 10(1), 55-61. https://hdl.handle.net/21.15107/rcub_vinar_2325
Tanić N, Dedović-Tanić N, Vujošević M, Dimitrijević BB. DNA profiling of B chromosomes from the yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia). in Genome Research. 2000;10(1):55-61. https://hdl.handle.net/21.15107/rcub_vinar_2325 .
Tanić, Nikola, Dedović-Tanić, Nasta, Vujošević, Mladen, Dimitrijević, Bogomir B., "DNA profiling of B chromosomes from the yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia)" in Genome Research, 10, no. 1 (2000):55-61, https://hdl.handle.net/21.15107/rcub_vinar_2325 .