Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy
Само за регистроване кориснике
2017
Аутори
Čabarkapa, AndreaDekanski, Dragana
Živković, Lada
Milanovic-Cabarkapa, Mirjana
Bajić, Vladan P.
Topalović, Dijana
Giampieri, Francesca
Gasparrini, Massimiliano
Battino, Maurizio
Spremo-Potparević, Biljana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations GT = 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significan...tly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy. (C) 2016 Elsevier Ltd. All rights reserved.
Кључне речи:
Lead (Pb) / Oxidative stress / DNA damage / Dry olive leaf extract (DOLE) / ChelationИзвор:
Food and Chemical Toxicology, 2017, 106, 616-623Финансирање / пројекти:
- Аберације ћелијског циклуса и утицај оксидативног стреса на неуродегенеративне процесе и малигну трансформацију ћелије (RS-MESTD-Basic Research (BR or ON)-173034)
- Republic of Serbia [680-00-566/2013-09/02]
- Republic of Italy [680-00-566/2013-09/02]
DOI: 10.1016/j.fct.2016.12.023
ISSN: 0278-6915; 1873-6351
PubMed: 28011361
WoS: 000407538200007
Scopus: 2-s2.0-85009230956
Колекције
Институција/група
VinčaTY - JOUR AU - Čabarkapa, Andrea AU - Dekanski, Dragana AU - Živković, Lada AU - Milanovic-Cabarkapa, Mirjana AU - Bajić, Vladan P. AU - Topalović, Dijana AU - Giampieri, Francesca AU - Gasparrini, Massimiliano AU - Battino, Maurizio AU - Spremo-Potparević, Biljana PY - 2017 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1679 AB - The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations GT = 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy. (C) 2016 Elsevier Ltd. All rights reserved. T2 - Food and Chemical Toxicology T1 - Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy VL - 106 SP - 616 EP - 623 DO - 10.1016/j.fct.2016.12.023 ER -
@article{ author = "Čabarkapa, Andrea and Dekanski, Dragana and Živković, Lada and Milanovic-Cabarkapa, Mirjana and Bajić, Vladan P. and Topalović, Dijana and Giampieri, Francesca and Gasparrini, Massimiliano and Battino, Maurizio and Spremo-Potparević, Biljana", year = "2017", abstract = "The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations GT = 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy. (C) 2016 Elsevier Ltd. All rights reserved.", journal = "Food and Chemical Toxicology", title = "Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy", volume = "106", pages = "616-623", doi = "10.1016/j.fct.2016.12.023" }
Čabarkapa, A., Dekanski, D., Živković, L., Milanovic-Cabarkapa, M., Bajić, V. P., Topalović, D., Giampieri, F., Gasparrini, M., Battino, M.,& Spremo-Potparević, B.. (2017). Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy. in Food and Chemical Toxicology, 106, 616-623. https://doi.org/10.1016/j.fct.2016.12.023
Čabarkapa A, Dekanski D, Živković L, Milanovic-Cabarkapa M, Bajić VP, Topalović D, Giampieri F, Gasparrini M, Battino M, Spremo-Potparević B. Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy. in Food and Chemical Toxicology. 2017;106:616-623. doi:10.1016/j.fct.2016.12.023 .
Čabarkapa, Andrea, Dekanski, Dragana, Živković, Lada, Milanovic-Cabarkapa, Mirjana, Bajić, Vladan P., Topalović, Dijana, Giampieri, Francesca, Gasparrini, Massimiliano, Battino, Maurizio, Spremo-Potparević, Biljana, "Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy" in Food and Chemical Toxicology, 106 (2017):616-623, https://doi.org/10.1016/j.fct.2016.12.023 . .