Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres
2017
Аутори
Živković, LadaAkar, Banu
Roux, Brianna M.
Spremo-Potparević, Biljana
Bajić, Vladan P.
Brey, Eric M.
Чланак у часопису (Објављена верзија)
,
©2016 WILEY PERIODICALS, INC.
Метаподаци
Приказ свих података о документуАпстракт
Poly (lactic-co-glycolic acid) (PLGA)-based materials are widely investigated for drug delivery and tissue engineering applications. Despite their popularity the genotoxic potential of PLGA has not been investigated. In this study, the comet assay, a sensitive assay for DNA damage, was used to evaluate potential genotoxicity in model cell types exposed to PLGA microspheres. Human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) cells were exposed to PLGA microspheres (0.4-6mg/mL) and DNA damage assessed at 24h, 4days, and 7days. DNA damage was not identified after 24h. However, after 4 and 7 days of exposure to 2 and 6mg/mL of PLGA microspheres a significant elevation of DNA damage in both cell types was observed. The PLGA microspheres did not exhibit any cytotoxic effects on the cells under the conditions tested. Our results suggest that PLGA may have a genotoxic effect on cells. A broader investigation of the PLGA genotoxic profile in biological systems is ...needed. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 284-291, 2017.
Кључне речи:
biomaterials / genotoxicity / HUVECs / MSCs / tissue engineeringИзвор:
Journal of Biomedical Materials Research. Part A, 2017, 105, 1, 284-291Финансирање / пројекти:
- Аберације ћелијског циклуса и утицај оксидативног стреса на неуродегенеративне процесе и малигну трансформацију ћелије (RS-MESTD-Basic Research (BR or ON)-173034)
- National Science Foundation [CBET-1263994], Veterans Administration [5 I01 BX000418-06], Fulbright Program
DOI: 10.1002/jbm.a.35849
ISSN: 1549-3296; 1552-4965
PubMed: 27480196
WoS: 000389145400029
Scopus: 2-s2.0-84995545687
Колекције
Институција/група
VinčaTY - JOUR AU - Živković, Lada AU - Akar, Banu AU - Roux, Brianna M. AU - Spremo-Potparević, Biljana AU - Bajić, Vladan P. AU - Brey, Eric M. PY - 2017 UR - https://vinar.vin.bg.ac.rs/handle/123456789/1324 AB - Poly (lactic-co-glycolic acid) (PLGA)-based materials are widely investigated for drug delivery and tissue engineering applications. Despite their popularity the genotoxic potential of PLGA has not been investigated. In this study, the comet assay, a sensitive assay for DNA damage, was used to evaluate potential genotoxicity in model cell types exposed to PLGA microspheres. Human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) cells were exposed to PLGA microspheres (0.4-6mg/mL) and DNA damage assessed at 24h, 4days, and 7days. DNA damage was not identified after 24h. However, after 4 and 7 days of exposure to 2 and 6mg/mL of PLGA microspheres a significant elevation of DNA damage in both cell types was observed. The PLGA microspheres did not exhibit any cytotoxic effects on the cells under the conditions tested. Our results suggest that PLGA may have a genotoxic effect on cells. A broader investigation of the PLGA genotoxic profile in biological systems is needed. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 284-291, 2017. T2 - Journal of Biomedical Materials Research. Part A T1 - Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres VL - 105 IS - 1 SP - 284 EP - 291 DO - 10.1002/jbm.a.35849 ER -
@article{ author = "Živković, Lada and Akar, Banu and Roux, Brianna M. and Spremo-Potparević, Biljana and Bajić, Vladan P. and Brey, Eric M.", year = "2017", abstract = "Poly (lactic-co-glycolic acid) (PLGA)-based materials are widely investigated for drug delivery and tissue engineering applications. Despite their popularity the genotoxic potential of PLGA has not been investigated. In this study, the comet assay, a sensitive assay for DNA damage, was used to evaluate potential genotoxicity in model cell types exposed to PLGA microspheres. Human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) cells were exposed to PLGA microspheres (0.4-6mg/mL) and DNA damage assessed at 24h, 4days, and 7days. DNA damage was not identified after 24h. However, after 4 and 7 days of exposure to 2 and 6mg/mL of PLGA microspheres a significant elevation of DNA damage in both cell types was observed. The PLGA microspheres did not exhibit any cytotoxic effects on the cells under the conditions tested. Our results suggest that PLGA may have a genotoxic effect on cells. A broader investigation of the PLGA genotoxic profile in biological systems is needed. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 284-291, 2017.", journal = "Journal of Biomedical Materials Research. Part A", title = "Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres", volume = "105", number = "1", pages = "284-291", doi = "10.1002/jbm.a.35849" }
Živković, L., Akar, B., Roux, B. M., Spremo-Potparević, B., Bajić, V. P.,& Brey, E. M.. (2017). Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres. in Journal of Biomedical Materials Research. Part A, 105(1), 284-291. https://doi.org/10.1002/jbm.a.35849
Živković L, Akar B, Roux BM, Spremo-Potparević B, Bajić VP, Brey EM. Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres. in Journal of Biomedical Materials Research. Part A. 2017;105(1):284-291. doi:10.1002/jbm.a.35849 .
Živković, Lada, Akar, Banu, Roux, Brianna M., Spremo-Potparević, Biljana, Bajić, Vladan P., Brey, Eric M., "Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres" in Journal of Biomedical Materials Research. Part A, 105, no. 1 (2017):284-291, https://doi.org/10.1002/jbm.a.35849 . .